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Home / Equine Research Bank / Virus Res / Mutations occurring during serial passage of Japanese equine...
Virus research2000; 68(1); 93-98; doi: 10.1016/s0168-1702(00)00147-7

Mutations occurring during serial passage of Japanese equine infectious anemia virus in primary horse macrophages.

Abstract: An attenuated equine infectious anemia virus (EIAV), named V26, was previously obtained after 50 passages of the Japanese virulent strain V70 in primary macrophage culture. To clarify the differences between both viruses, their full-length sequences were determined. There were higher mutations in S2 (6.15% amino acid difference) and LTR (10.7% nucleotide difference). The presumed initiation codon of the S2 gene was absent from the sequence of V26. There was a large insertion within the long-terminal repeat (LTR) U3 hypervariable region of V26. In addition, there were minor mutations in gag (1.22% amino acid difference), pol (1.05% amino acid difference) and env (1. 65% amino acid difference) regions, but no mutation in tat region. No mutations were observed in the principal neutralizing domain in the gp90. Thus, the mutations in the S2 and LTR might be the major target sites of mutation in EIAV during serial passages in vitro.
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Publication Date: 2000-08-10 PubMed ID: 10930666DOI: 10.1016/s0168-1702(00)00147-7Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study compares the genetic differences between an infectious equine virus strain and its attenuated variant, showing most significant mutations in the S2 and LTR regions of the virus, which may be the primary mutation sites during serial passages in the lab.

Attenuation of Japanese EIAV through Serial Passage

  • A Japanese equine infectious anemia virus (EIAV) strain V70 was attenuated into a less harmful version called V26.
  • This process involved 50 passages of the virus through primary horse macrophage cultures, which is essentially exposing cells to the virus sequentially to reduce the virus’s severity by allowing it to adapt to growing in the lab instead of a horse.

Comparing Full-length Sequences of Virulent and Attenuated EIAV

  • The researchers did a full-length sequence analysis of both viruses to identify and analyze the genetic differences.
  • The S2 region and the LTR (long terminal repeat) region of the virus showed the most pronounced mutations.
  • The S2 region displayed an amino acid difference of 6.15%, and the initiation codon for this gene was absent in V26.
  • The LTR region showed a nucleotide difference of 10.7%, with a notably large insertion within the U3 hypervariable segment in V26.

Observations on Other Region Mutations

  • minor mutations were observed in the gag (1.22% amino acid difference), pol (1.05% amino acid difference), and env (1.65% amino acid difference) regions.
  • No mutations were spotted in the tat region, and there were no observable mutations in the principal neutralizing domain in the gp90 protein.

Implication of S2 and LTR Mutations

  • The most crucial mutations were in the S2 and LTR regions of the virus, indicating that these might be the significant mutation sites during serial passages for this virus in the lab.
  • The higher susceptibility of these sections to mutate could have implications for how the virus adapts to different conditions, potentially playing a role in its attenuation.

Cite This Article

APA
Zheng YH, Sentsui H, Kono Y, Ikuta K. (2000). Mutations occurring during serial passage of Japanese equine infectious anemia virus in primary horse macrophages. Virus Res, 68(1), 93-98. https://doi.org/10.1016/s0168-1702(00)00147-7

Publication

Virus research
ISSN: 0168-1702
NlmUniqueID: 8410979
Country: Netherlands
Language: English
Volume: 68
Issue: 1
Pages: 93-98

Researcher Affiliations

Zheng, Y H
  • National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
Sentsui, H
    Kono, Y
      Ikuta, K

        MeSH Terms

        • Amino Acid Sequence
        • Animals
        • Cells, Cultured
        • Genes, Viral
        • Genes, gag
        • Genes, pol
        • Genes, tat
        • Horses
        • Infectious Anemia Virus, Equine / genetics
        • Infectious Anemia Virus, Equine / pathogenicity
        • Infectious Anemia Virus, Equine / physiology
        • Macrophages / virology
        • Molecular Sequence Data
        • Mutation
        • Polymerase Chain Reaction
        • Sequence Analysis, DNA
        • Serial Passage
        • Terminal Repeat Sequences / genetics
        • Viral Proteins / genetics

        Citations

        This article has been cited 10 times.
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          doi: 10.1007/s11262-016-1382-2pubmed: 27572122google scholar: lookup
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          doi: 10.3390/v5122963pubmed: 24316675google scholar: lookup
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          doi: 10.1007/s11262-010-0563-7pubmed: 21369830google scholar: lookup
        8. Craigo JK, Barnes S, Zhang B, Cook SJ, Howe L, Issel CJ, Montelaro RC. An EIAV field isolate reveals much higher levels of subtype variability than currently reported for the equine lentivirus family.. Retrovirology 2009 Oct 20;6:95.
          doi: 10.1186/1742-4690-6-95pubmed: 19843328google scholar: lookup
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          doi: 10.1128/JVI.79.9.5653-5664.2005pubmed: 15827180google scholar: lookup
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