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Myeloperoxidase activity of the large intestine in an equine model of acute colitis.
Abstract: To determine whether quantification of myeloperoxidase (MPO) activity could be a useful laboratory technique to detect granulocyte infiltration in equine intestinal tissues. Methods: Intestinal tissue (inflamed or healthy) collected from 16 age- and sex-matched Shetland Ponies. Methods: Intestinal tissue MPO activity was determined, and histologic assessment of adjacent specimens from healthy and inflamed intestine was done. Results: Intestinal tissue MPO activity and histopathologic score increased with time after castor oil challenge and peaked at 16 hours in an equine diarrhea model in which individual ponies provided their own control tissues. Conclusions: Intestinal tissue inflammation scores correlated positively with tissue MPO activity in adjacent specimens. Conclusions: Tissue MPO assay may be a useful laboratory tool to quantify intestinal mucosal inflammation in ponies.
Publication Date: 1999-07-17 PubMed ID: 10407471 The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research investigates if measuring myeloperoxidase (MPO) activity can be an effective way to detect granulocyte infiltration in horse intestinal tissues, using 16 Shetland Ponies as a study model. The findings suggest that MPO assays could indeed be a useful tool in quantifying intestinal mucosal inflammation in ponies.
Objective and Methodology
- The primary aim of the research was to examine whether myeloperoxidase (MPO), an enzyme found in neutrophils (a type of white blood cell), could serve as an effective tool to detect granulocyte infiltration in equine intestinal tissues. This was done in the context of acute colitis, a condition characterized by inflammation of the colon.
- The researchers conducted their study on 16 Shetland Ponies, specifically chosen due to their age and sex. This experimental model allowed the researchers to study the effects of acute colitis under controlled conditions and among a homogenous group.
- To induce symptoms of colitis, the ponies were given a castor oil challenge, which is a commonly used method in veterinary science to trigger gastrointestinal reactions.
Results and Findings
- The study determined MPO activity using intestinal tissue samples and proceeded with a histologic assessment of both healthy and inflamed intestinal specimens.
- It was found that the level of MPO activity and the histopathologic score, which evaluates the severity of the histological abnormalities, increased with time following the castor oil challenge.
- The peak of MPO activity was observed 16 hours after the challenge. This was reflected in the ponies’ diarrheal response, illustrating the relationship between MPO activity, granulocyte infiltration and intestinal inflammation.
Conclusions
- The research concluded that there is a positive correlation between intestinal tissue inflammation scores and tissue MPO activity. This means that an increase in MPO activity could indicate a higher degree of intestinal inflammation.
- Based on the results, the study suggested that the MPO assay might serve as a practical laboratory tool to quantify intestinal mucosal inflammation in ponies. This could help in the diagnosis of acute colitis and the management of disease progression.
Cite This Article
APA
McConnico RS, Weinstock D, Poston ME, Roberts MC.
(1999).
Myeloperoxidase activity of the large intestine in an equine model of acute colitis.
Am J Vet Res, 60(7), 807-813.
Publication
Researcher Affiliations
- Department of Food Animal and Equine Medicine, College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA.
MeSH Terms
- Animals
- Benzidines / chemistry
- Castor Oil / adverse effects
- Cecum / pathology
- Chromogenic Compounds / chemistry
- Colitis, Ischemic / diagnosis
- Colitis, Ischemic / physiopathology
- Colitis, Ischemic / veterinary
- Colon / pathology
- Disease Models, Animal
- Female
- Horse Diseases / physiopathology
- Horses
- Ileum / pathology
- Inflammation / veterinary
- Intestinal Mucosa / enzymology
- Intestinal Mucosa / pathology
- Intestine, Large / enzymology
- Intestine, Large / pathology
- Male
- Peroxidase / chemistry
Citations
This article has been cited 11 times.- Grizer CS, Elliott KC, Singh G, Vogt MR, Mattapallil JJ. Respiratory infection with Enterovirus D68 induces severe acute lung inflammation in the pediatric ferret model. iScience 2025 Aug 15;28(8):113071.
- Kvidera SK, Mayorga EJ, McCarthy CS, Horst EA, Abeyta MA, Baumgard LH. Effects of supplemental citrulline on thermal and intestinal morphology parameters during heat stress and feed restriction in growing pigs. J Anim Sci 2024 Jan 3;102.
- Kaji N, Iwaoka K, Nakamura S, Tsukamoto A. Fuzapladib reduces postsurgical inflammation in the intestinal muscularis externa. J Vet Med Sci 2023 Nov 2;85(11):1151-1156.
- Gao C, Zhou Y, Chen Z, Li H, Xiao Y, Hao W, Zhu Y, Vong CT, Farag MA, Wang Y, Wang S. Turmeric-derived nanovesicles as novel nanobiologics for targeted therapy of ulcerative colitis. Theranostics 2022;12(12):5596-5614.
- Yu QQ, Zhang H, Guo Y, Han B, Jiang P. The Intestinal Redox System and Its Significance in Chemotherapy-Induced Intestinal Mucositis. Oxid Med Cell Longev 2022;2022:7255497.
- Amanzada A, Malik IA, Nischwitz M, Sultan S, Naz N, Ramadori G. Myeloperoxidase and elastase are only expressed by neutrophils in normal and in inflamed liver. Histochem Cell Biol 2011 Mar;135(3):305-15.
- Faleiros RR, Macoris DG, Alves GE, Souza DG, Teixeira MM, Moore RM. Local and remote lesions in horses subjected to small colon distension and decompression. Can J Vet Res 2008 Jan;72(1):68-76.
- Grulke S, Franck T, Gangl M, Péters F, Salciccia A, Deby-Dupont G, Serteyn D. Myeloperoxidase assay in plasma and peritoneal fluid of horses with gastrointestinal disease. Can J Vet Res 2008 Jan;72(1):37-42.
- Art T, Franck T, Lekeux P, de Moffarts B, Couëtil L, Becker M, Kohnen S, Deby-Dupont G, Serteyn D. Myeloperoxidase concentration in bronchoalveolar lavage fluid from healthy horses and those with recurrent airway obstruction. Can J Vet Res 2006 Oct;70(4):291-6.
- Milo LA, Reardon KA, Tappenden KA. Effects of short-chain fatty acid-supplemented total parenteral nutrition on intestinal pro-inflammatory cytokine abundance. Dig Dis Sci 2002 Sep;47(9):2049-55.
- McConnico RS, Argenzio RA, Roberts MC. Prostaglandin E2 and reactive oxygen metabolite damage in the cecum in a pony model of acute colitis. Can J Vet Res 2002 Jan;66(1):50-4.
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