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Canadian journal of veterinary research = Revue canadienne de recherche veterinaire2008; 72(1); 37-42;

Myeloperoxidase assay in plasma and peritoneal fluid of horses with gastrointestinal disease.

Abstract: Gastrointestinal disorders, especially strangulating intestinal obstructions, are still a major cause of illness and death in the horse. Circulating lipopolysaccharides may activate both neutrophils and monocytes. The activated neutrophils release myeloperoxidase (MPO), a specific enzyme with strong oxidative activity. The aim of this study was to evaluate MPO concentrations in the plasma and peritoneal fluid (PF) of horses with colic and to check the hypothesis that these concentrations would be higher in a case of strangulating obstruction than in cases of nonstrangulating disease. By using a specific enzyme-linked immunosorbent assay for equine MPO, we determined the MPO concentrations in horses admitted to a clinic for colic. Horses with nonstrangulating or strangulating obstruction of the large intestine (NSLI or SLI), strangulating obstruction of the small intestine (SSI), or inflammatory bowel disease (IBD) were compared with healthy horses. The horses with SLI, SSI, or IBD had significantly higher MPO levels in plasma and PF than did those in the other 2 groups. The mean plasma level was significantly higher in the horses with NSLI than in the healthy horses. High MPO values in PF indicated necrotic bowel. These results show that neutrophil activation occurs during nonstrangulating and strangulating intestinal obstruction in horses and that the plasma and PF MPO concentrations may be a marker of the severity of the disease. Les pathologies intestinales du cheval, en particulier les obstructions étranglées, sont toujours une cause majeure de morbidité et de mortalité chez le cheval. A côté de l’activation des monocytes via les lipopolysaccharides, une activation des neutrophiles se produit également. Le neutrophile activé relargue la myéloperoxidase (MPO), une enzyme spécifique qui a une activité oxidative puissante. Le but de l’étude est d’évaluer le taux de MPO du plasma et du liquide péritonéal chez des chevaux en coliques à l’admission. L’hypothèse est que les taux de MPO sont plus importants en cas d’obstruction étranglée par rapport à l’obstruction simple. En utilisant un test de dosage immunoenzymatique spécifique de la MPO équine, les auteurs ont déterminé les taux de MPO dans le plasma et le liquide péritonéal des chevaux admis pour colique en clinique. Quatre groupes de chevaux ont été étudiés et comparés aux chevaux sains : des chevaux avec obstruction non-étranglée ou étranglée du gros intestin, obstruction étranglée de l’intestin grêle, et pathologie intestinale de type inflammatoire. Les chevaux avec pathologie étranglée et inflammatoire présentent des taux de MPO du plasma et du liquide péritonéal significativement plus élevés que les chevaux avec pathologie non-étranglée et les sains. Les valeurs de MPO plasmatique sont significativement plus élevées chez les chevaux avec pathologie non-étranglée par rapport aux chevaux sains. Des valeurs élevées de MPO dans le liquide péritonéal sont des indices de nécrose intestinale. Ces résultats montrent l’existence d’une activation des neutrophiles lors des pathologies intestinales étranglées et non-étranglées chez le cheval. La concentration de MPO dans le plasma et le liquide péritonéal peut être un indicateur de la gravité de la pathologie intestinale chez le cheval. (Traduit par les auteurs)
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Publication Date: 2008-01-25 PubMed ID: 18214160PubMed Central: PMC2117365
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research explores the concentration levels of myeloperoxidase (MPO) enzyme in the blood and peritoneal fluid of horses suffering from colic, aiming to investigate if these levels increase in cases of intestinal obstruction. It concludes that MPO concentrations can be used to indicate the severity of the disease.

Objective of the Research

  • The primary goal of this research was to examine the concentration levels of Myeloperoxidase (MPO) enzyme in the blood and peritoneal fluid of horses that are suffering from colic.
  • The study also aimed to test if these levels were higher in strangulating obstruction, a severe form of the disease, compared to non-strangulating disease states.
  • The potential of MPO concentrations as a biomarker for assessing the severity of the disease was also investigated.

Methodology of the Research

  • The study involved comparing horses with different conditions: nonstrangulating obstruction of the large intestine (NSLI), strangulating obstruction of the large intestine (SLI), strangulating obstruction of the small intestine (SSI), inflammatory bowel disease (IBD), and healthy horses.
  • A specific enzyme-linked immunosorbent assay was utilized to measure the levels of MPO.

Findings of the Research

  • The findings indicated that MPO levels in plasma and peritoneal fluid were significantly higher in horses suffering from SLI, SSI, or IBD compared to the other groups.
  • Moreover, horses with NSLI had a significantly higher mean plasma level of MPO than the healthy horses.
  • High MPO values in the peritoneal fluid were found to suggest necrotic bowel, a severe condition of tissue death due to inadequate blood supply.

Conclusion of the Research

  • The results demonstrated neutrophil activation in both nonstrangulating and strangulating intestinal obstruction in horses.
  • MPO concentrations in the plasma and peritoneal fluid, thus, may serve as a marker to indicate the severity of the disease, providing healthcare professionals a potential diagnostic tool.

Cite This Article

APA
Grulke S, Franck T, Gangl M, Péters F, Salciccia A, Deby-Dupont G, Serteyn D. (2008). Myeloperoxidase assay in plasma and peritoneal fluid of horses with gastrointestinal disease. Can J Vet Res, 72(1), 37-42.

Publication

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire
ISSN: 0830-9000
NlmUniqueID: 8607793
Country: Canada
Language: English
Volume: 72
Issue: 1
Pages: 37-42

Researcher Affiliations

Grulke, Sigrid
  • Clinique Equine, Anesthésiologie et Pathologie chirurgicale, Faculté de médecine vétérinaire B 41, Université de Liège, Sart Tilman, B-4000 Liège, Belgium. sgrulke@ulg.ac.be
Franck, Thierry
    Gangl, Monika
      Péters, Fabrice
        Salciccia, Alexandra
          Deby-Dupont, Ginette
            Serteyn, Didier

              MeSH Terms

              • Animals
              • Ascitic Fluid / enzymology
              • Biomarkers / analysis
              • Biomarkers / blood
              • Case-Control Studies
              • Colic / blood
              • Colic / enzymology
              • Colic / veterinary
              • Female
              • Gastrointestinal Diseases / blood
              • Gastrointestinal Diseases / enzymology
              • Gastrointestinal Diseases / veterinary
              • Horse Diseases / blood
              • Horse Diseases / enzymology
              • Horses
              • Inflammatory Bowel Diseases / blood
              • Inflammatory Bowel Diseases / enzymology
              • Inflammatory Bowel Diseases / veterinary
              • Intestinal Obstruction / blood
              • Intestinal Obstruction / enzymology
              • Intestinal Obstruction / veterinary
              • Male
              • Neutrophil Activation
              • Neutrophils / enzymology
              • Peroxidase / analysis
              • Peroxidase / blood
              • Severity of Illness Index

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              Citations

              This article has been cited 6 times.
              1. Birckhead EM, Das S, Tidd N, Raidal SL, Raidal SR. Visualizing neutrophil extracellular traps in septic equine synovial and peritoneal fluid samples using immunofluorescence microscopy. J Vet Diagn Invest 2023 Nov;35(6):751-760.
                doi: 10.1177/10406387231196552pubmed: 37661696google scholar: lookup
              2. Parrilla Hernández S, Franck T, Munaut C, Feyereisen É, Piret J, Farnir F, Reigner F, Barrière P, Deleuze S. Characterization of Myeloperoxidase in the Healthy Equine Endometrium. Animals (Basel) 2023 Jan 21;13(3).
                doi: 10.3390/ani13030375pubmed: 36766264google scholar: lookup
              3. Shaeib F, Khan SN, Thakur M, Kohan-Ghadr HR, Drewlo S, Saed GM, Pennathur S, Abu-Soud HM. The Impact of Myeloperoxidase and Activated Macrophages on Metaphase II Mouse Oocyte Quality. PLoS One 2016;11(3):e0151160.
                doi: 10.1371/journal.pone.0151160pubmed: 26982351google scholar: lookup
              4. Derochette S, Mouithys-Mickalad A, Franck T, Collienne S, Ceusters J, Deby-Dupont G, Neven P, Serteyn D. NDS27 combines the effect of curcumin lysinate and hydroxypropyl-β-cyclodextrin to inhibit equine PKCδ and NADPH oxidase involved in the oxidative burst of neutrophils. FEBS Open Bio 2014;4:1021-9.
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              5. Moeinian M, Ghasemi-Niri SF, Mozaffari S, Abdolghaffari AH, Baeeri M, Navaea-Nigjeh M, Abdollahi M. Beneficial effect of butyrate, Lactobacillus casei and L-carnitine combination in preference to each in experimental colitis. World J Gastroenterol 2014 Aug 21;20(31):10876-85.
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              6. Rahimi R, Baghaei A, Baeeri M, Amin G, Shams-Ardekani MR, Khanavi M, Abdollahi M. Promising effect of Magliasa, a traditional Iranian formula, on experimental colitis on the basis of biochemical and cellular findings. World J Gastroenterol 2013 Mar 28;19(12):1901-11.
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