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Home / Equine Research Bank / Can J Vet Res / Myeloperoxidase concentration in bronchoalveolar lavage flui...
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire2006; 70(4); 291-296;

Myeloperoxidase concentration in bronchoalveolar lavage fluid from healthy horses and those with recurrent airway obstruction.

Abstract: The aim of this work was to measure the myeloperoxidase (MPO) concentration in bronchoalveolar lavage (BAL) fluid collected from horses with recurrent airway obstruction (RAO), both in crisis and in remission, as well as from healthy horses. Seven horses with RAO were exposed to moldy hay until the maximum change in pleural pressure was greater than 1.5 kPa. At that point, BAL was performed, and the total cell counts and percentages in the fluid were immediately determined. To measure the MPO concentration in BAL-fluid supernatant, we used a specific enzyme-linked immunosorbent assay with polyclonal antibodies against equine MPO. The tests were repeated on the horses with RAO after they had spent 2 mo on pasture. Six healthy horses serving as controls underwent the same tests. The absolute and relative neutrophil counts and the MPO concentration in the BAL fluid were significantly greater in the horses with an RAO crisis than in the control horses. After 2 mo on pasture, the horses that had been in RAO crisis were clinically normal, and their neutrophil counts and MPO levels in BAL fluid had significantly decreased; during remission their neutrophil counts were not significantly different from those in the healthy horses, but their MPO concentration remained significantly higher. This study showed that determining the MPO concentration in a horse's BAL fluid is technically possible and that during remission from RAO the concentration remains higher than normal. Thus, MPO may be a marker of neutrophil presence and activation in the lower airways. Le but de cette étude était de mesurer la concentration de myéloperoxydase (MPO) dans le liquide de lavage broncho-alvéolaire (BAL) récolté chez des chevaux avec obstruction respiratoire récurrente (RAO), en période de crise et en rémission, ainsi que chez des chevaux en santé. Sept chevaux avec RAO ont été exposés à du foin moisi jusqu’à ce que le changement maximal dans la pression pleurale soit plus grand que 1,5 kPa. À cet instant on procéda au BAL et le dénombrement cellulaire total de même que les pourcentages dans le liquide furent immédiatement déterminés. Afin de mesurer la concentration de MPO dans le liquide de BAL on procéda à une épreuve immuno-enzymatique spécifique utilisant des anticorps polyclonaux dirigés contre MPO. Les épreuves ont été répétées chez les chevaux avec RAO après qu’ils eurent passé 2 mois au pâturage. Six chevaux en santé servant de témoin ont subi les mêmes tests. Les comptes absolus et relatifs de neutrophiles et les concentrations de MPO dans le liquide de BAL étaient significativement plus élevés chez les chevaux avec RAO en période de crise que chez les chevaux témoins. Après 2 mois au pâturage, les chevaux qui avaient été en crise de RAO étaient cliniquement normaux et leurs comptes en neutrophiles et niveaux de MPO dans le liquide de BAL avaient diminué de manière significative; durant la rémission les comptes de neutrophiles n’étaient pas significativement différents de ceux des chevaux témoins, mais les concentrations de MPO sont demeurées significativement plus élevées. Cette étude a permis de démontrer qu’il était techniquement possible de déterminer la concentration de MPO dans du liquide de BAL équin et que durant la période de rémission d’une crise de RAO la concentration est demeurée plus élevée que la normale. Ainsi, la MPO pourrait être un marqueur de la présence et de l’activation des neutrophiles dans les voies respiratoires inférieures. (Traduit par Docteur Serge Messier)
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Publication Date: 2006-10-18 PubMed ID: 17042382PubMed Central: PMC1562538
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the concentration of myeloperoxidase (MPO), a substance typically present during inflammation, in horses with recurrent airway obstruction, both in active disease and in remission, and compares it to healthy horses. The findings suggest MPO potentially serving as an indicator for the presence and activation of a type of white blood cell, neutrophils, in the lower airways of horses.

Research Goal

  • The main objective of this study was to determine the concentration of myeloperoxidase (MPO) in bronchoalveolar lavage (BAL) fluid of healthy horses and those suffering from recurrent airway obstruction (RAO). The study was conducted during both crisis phases and remission periods in the RAO affected horses.

Research Methodology

  • The study includes seven horses with RAO that were exposed to moldy hay until their pleural pressure increased significantly. At this time, BAL was conducted, and researchers recorded the total cell counts and percentages in the obtained fluid.
  • A specific assay using polyclonal antibodies against horse MPO was utilized to measure MPO concentration in the BAL fluid supernatants.
  • The horses with RAO were then sent to graze for two months, post which the tests were repeated. Concurrently, six healthy horses were subjected to the same tests as control subjects.

Findings

  • During the RAO crisis, the affected horses showed significantly greater neutrophil counts and MPO concentration in their BAL fluid than the control horses.
  • Upon spending two months on pasture, the afflicted horses reverted to a clinically normal state, and their neutrophil counts and MPO levels in BAL fluid dropped substantially. However, despite showing neutrophil counts similar to the healthy horses during remission, their MPO concentration remained significantly high.
  • The results also demonstrated that it’s technically feasible to determine the MPO concentration in horse’s BAL fluid.

Conclusion

  • The outcome of the study indicates that MPO might serve as a marker for the presence and activation of neutrophils in the lower airways. Thus, measuring MPO concentrations could be a useful diagnostic tool for assessing the severity or status of RAO in horses.

Cite This Article

APA
Art T, Franck T, Lekeux P, de Moffarts B, Couëtil L, Becker M, Kohnen S, Deby-Dupont G, Serteyn D. (2006). Myeloperoxidase concentration in bronchoalveolar lavage fluid from healthy horses and those with recurrent airway obstruction. Can J Vet Res, 70(4), 291-296.

Publication

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire
ISSN: 0830-9000
NlmUniqueID: 8607793
Country: Canada
Language: English
Volume: 70
Issue: 4
Pages: 291-296

Researcher Affiliations

Art, Tatiana
  • Equine Sports Medicine Centre, Faculty of Veterinary Medicine, University of Liege, Belgium. Tatiana.art@ulg.ac.be
Franck, Thierry
    Lekeux, Pierre
      de Moffarts, Brieuc
        Couëtil, Laurent
          Becker, Martine
            Kohnen, Serge
              Deby-Dupont, Ginette
                Serteyn, Didier

                  MeSH Terms

                  • Animals
                  • Antibodies
                  • Biomarkers / metabolism
                  • Blood Gas Analysis / veterinary
                  • Bronchoalveolar Lavage / methods
                  • Bronchoalveolar Lavage / veterinary
                  • Bronchoalveolar Lavage Fluid / cytology
                  • Case-Control Studies
                  • Cell Count / veterinary
                  • Enzyme-Linked Immunosorbent Assay / methods
                  • Enzyme-Linked Immunosorbent Assay / veterinary
                  • Horse Diseases / enzymology
                  • Horses
                  • Lung Diseases, Obstructive / enzymology
                  • Lung Diseases, Obstructive / veterinary
                  • Neutrophils / immunology
                  • Peroxidase / analysis
                  • Peroxidase / blood
                  • Respiratory Function Tests / veterinary

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