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Home / Equine Research Bank / Equine Vet J / Myenteric networks of interstitial cells of Cajal are reduce...
Equine veterinary journal2019; 52(2); 298-304; doi: 10.1111/evj.13160

Myenteric networks of interstitial cells of Cajal are reduced in horses with inflammatory bowel disease.

Abstract: Inflammatory bowel disease (IBD) is a well-recognised but poorly understood disease complex in the horse. Clinical signs may vary but often include weight loss, diarrhoea and colic. The effect this disease process may have on the gastrointestinal pacemaker cells (the interstitial cells of Cajal), enteric neurons and glial cells has not been previously evaluated in the horse. Objective: To compare the density of the interstitial cells of Cajal (ICC), enteric neurons and glial cells in horses with IBD to those of normal horses using immunohistochemical markers. Methods: Retrospective, quantitative immunohistochemical study. Methods: Ileal samples were collected during post-mortem examinations from 14 horses with a clinical and histopathological diagnosis of IBD and from eight normal controls. All horses were Standardbreds 1-15 years of age. Six of the IBD cases had eosinophilic gastroenteritis (EG) while the remaining eight had granulomatous enteritis (GE). Tissue sections were labelled with anti-CD117 (c-Kit), anti-TMEM16 (TMEM16), anti-protein gene product (PGP9.5) and anti-glial fibrillary acidic protein (GFAP) using standard immunohistochemical labelling techniques. Image analysis was performed to quantify the presence of ICC (CD117, TMEM16) as well as neuronal (PGP9.5) and enteroglial (GFAP) networks. Results: Interstitial cells of Cajal networks were significantly reduced in the myenteric plexus (MP) region in IBD horses compared with the controls for both markers (P<0.05). There was no significant difference in the density of the neuronal or glial cell markers between the two groups (P>0.05). Conclusions: The number of horses included in the study. Conclusions: Disruption to ICC networks may contribute to the clinical signs of colic in some horses with IBD. Further studies are needed to establish the pathophysiological mechanisms involved and the functional effects of the reduced ICC networks.
© 2019 The Authors. Equine Veterinary Journal published by John Wiley & Sons Ltd on behalf of EVJ Ltd.
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Publication Date: 2019-09-05 PubMed ID: 31397916DOI: 10.1111/evj.13160Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research examines the impact of Inflammatory Bowel Disease (IBD) in horses on gastrointestinal pacemaker cells (interstitial cells of Cajal), enteric neurons, and glial cells. Results reveal a significant reduction of interstitial cells of Cajal in affected horses, potentially contributing to clinical symptoms like colic.

Objective of the Study

  • The study aimed to examine the density of interstitial cells of Cajal (ICCs), enteric neurons, and glial cells in horses with IBD and compare it with normal horses. This was achieved using specific immunohistochemical markers.

Research Methodology

  • As a retrospective, quantitative immunohistochemical study, it utilized ileal samples taken during post-mortem examinations of 14 horses diagnosed with IBD and 8 control horses.
  • The IBD cases consisted of six horses with eosinophilic gastroenteritis (EG) and eight with granulomatous enteritis (GE).
  • The selected tissue samples were then labelled with anti-CD117 (c-Kit), anti-TMEM16 (TMEM16), protein gene product (PGP9.5), and anti-glial fibrillary acidic protein (GFAP), following standard immunohistochemical protocols.
  • Image analysis was then utilized to quantify the presence of ICCs (using CD117 and TMEM16), as well as neuronal (PGP9.5) and enteroglial (GFAP) networks.

Key Findings

  • The results showed a significant decrease in the networks of the interstitial cells of Cajal located in the myenteric plexus region of horses with IBD when compared with the control horses.
  • In contrast, the density of neuronal or glial cell markers did not show a significant difference between the two groups.

Conclusions and Implications

  • The findings suggest that disruptions to the ICC networks could be a contributing factor to symptoms such as colic in horses suffering from IBD.
  • However, the exact pathophysiological mechanisms involved in this process, and the functional effects of reduced ICC networks, still need to be established through further studies.

Cite This Article

APA
Fintl C, Lindberg R, McL Press C. (2019). Myenteric networks of interstitial cells of Cajal are reduced in horses with inflammatory bowel disease. Equine Vet J, 52(2), 298-304. https://doi.org/10.1111/evj.13160

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 52
Issue: 2
Pages: 298-304

Researcher Affiliations

Fintl, C
  • Norwegian University of Life Sciences, Oslo, Norway.
Lindberg, R
  • Swedish University of Agricultural Sciences, Uppsala, Sweden.
McL Press, C
  • Norwegian University of Life Sciences, Oslo, Norway.

MeSH Terms

  • Animals
  • Colic / veterinary
  • Horse Diseases
  • Horses
  • Inflammatory Bowel Diseases / veterinary
  • Interstitial Cells of Cajal
  • Myenteric Plexus
  • Retrospective Studies

Grant Funding

  • Agricultural Agreement Research Fund
  • Norsk Rikstoto
  • Research Council of Norway
  • 41519 / Norwegian/Swedish research collaboration

References

This article includes 33 references
  1. Schumacher J, Edwards JF, Cohen ND. Chronic idiopathic inflammatory bowel disease of the horse.. J. Vet. Intern. Med. 2000;14, 258-265.
  2. Mair TS, Pearson GR, Divers TJ. Malabsorption syndromes in the horse.. Equine Vet. Educ. 2006;18, 299-308.
  3. Mäkinen PE, Archer DC, Baptiste KE, Malbon A, Proudman CJ, Kipar A. Characterisation of the inflammatory reaction in equine idiopathic focal eosinophilic enteritis and diffuse eosinophilic enteritis.. Equine Vet. J. 2008;40, 386-392.
  4. Lindberg R. Pathology of equine granulomatous enteritis.. J. Comp. Path. 1984;94, 233-247.
  5. Sanders KM, Koh SD, Ward SM. Interstitial cells of cajal as pacemakers in the gastrointestinal tract.. Annu. Rev. Physiol. 2006;68, 307-343.
  6. Burns GA, Karcher LF, Cummings JF. Equine myenteric ganglionitis: a case of chronic intestinal pseudo-obstruction.. Cornell Vet. 1990;80, 53-63.
  7. Doxey DL, Milne EM, Woodman MP, Gilmour JS, Chisholm HK. Small intestine and small colon neuropathy in equine dysautonomia (grass sickness).. Vet. Res. Commun. 1995;19, 529-543.
  8. Schusser GW, White NA. Morphologic and quantitative evaluation of the myenteric plexus and neurons in the large colon of horses.. J. Am. Vet. Med. Assoc. 1997;210, 928-934.
  9. Schusser GF, Scheidemann W, Huskamp B. Muscle thickness and neuron density in the caecum of horses with chronic recurrent caecal impaction.. Equine Vet. J. 2000;32, Suppl. 32, 69-73.
  10. Fintl C, Hudson NPH, Mayhew IG, Edwards GB, Proudman CJ, Pearson GT. The interstitial cells of Cajal (ICC) in equine colic: an immunohistochemical study of horses with obstructive disorders of the small and large intestines.. Equine Vet. J. 2004;36, 474-479.
  11. Pavone S, Gialetti R, Pepe M, Onofri A, Mandara MT. Histological and immunohistochemical studies of changes in myenteric plexuses and in interstitial cells of Cajal associated with equine colic.. Res. Vet. Sci. 2012;93, 350-359.
  12. Rumessen JJ. Ultrastructure of interstitial cells of Cajal at the colonic submuscular border in patients with ulcerative colitis.. Gastroenterology. 1996;111, 1447-1455.
  13. Porcher C, Baldo M, Henry M, Orsoni P, Julé Y, Ward SM. Deficiency of interstitial cells of Cajal in the small intestine of patients with Crohn's disease.. Am. J. Gastroent. 2002;97, 118-125.
  14. Ohlsson B, Veress B, Lindgren S, Sundkvist G. Enteric ganglioneuritis and abnormal interstitial cells of Cajal: features of inflammatory bowel disease.. Inflamm. Bowel Dis. 2007;13, 721-726.
  15. Schneider CA, Rasband WS, Eliceiri KW. NIH Image to ImageJ: 25 years of image analysis.. Nat. Methods 2012;9, 671-675.
  16. Ruifrok AC, Johnston DA. Quantification of histochemical staining by color deconvolution.. Anal. Quant. Cytol. Histol. 2001;23, 291-299.
  17. Cimprich RE. Equine granulomatous enteritis.. Vet. Pathol. 1974;11, 535-547.
  18. Merritt AM, Cimprich RE, Beech J. Granulomatous enteritis in nine horses.. J. Am. Vet. Med. Assoc. 1976;169, 603-609.
  19. Hudson NP, Pearson GT, Mayhew IG, Proudman CJ, Burden FA, Fintl C. Expression of PGP 9.5 by enteric neurons in horses and donkeys with and without intestinal disease.. J. Comp. Pathol. 2014;150, 225-233.
  20. Geboes K, Collins S. Structural abnormalities of the nervous system in Crohn's disease and ulcerative colitis.. Neurogastroenterol. Motil. 1998;10, 189-202.
  21. Wang XY, Berezin I, Mikkelsen HB, Der T, Bercik P, Collins SM, Huizinga JD. Pathology of interstitial cells of Cajal in relation to inflammation revealed by ultrastructure but not immunohistochemistry.. Am. J. Path. 2002;160, 1529-1540.
  22. Kaji N, Horiguchi K, Iino S, Nakayama S, Ohwada T, Otani Y, Firman Murata T, Sanders KM, Ozaki H, Hori M. Nitric oxide-induced oxidative stress impairs pacemaker function of murine interstitial cells of Cajal during inflammation.. Pharmacol. Res. 2016;111, 838-848.
  23. Lisowski ZM. Targeting the equine macrophage in equine post-operative ileus.. Chapter 5, Edinburgh University. PhD Thesis .
  24. Neurath MF. Cytokines in inflammatory bowel disease.. Nat. Rev. Immunol. 2014;14, 329-342.
  25. Espinosa I, Lee CH, Kim MK, Rouse BT, Subramanian S, Montgomery K, Varma S, Corless CL, Heinrich MC, Smith KS, Wang Z, Rubin B, Nielsen TO, Seitz RS, Ross DT, West RB, Cleary ML, van de Rijn M. A novel monoclonal antibody against DOG1 is a sensitive and specific marker for gastrointestinal stromal tumors.. Am. J. Surg. Pathol. 2008;32, 210-218.
  26. Hwang SJ, Blair PJ, Britton FC, O'Driscoll KE, Hennig G, Bayguinov YR, Rock JR, Harfe BD, Sanders KM, Ward SM. Expression of anoctamin 1/TMEM16A by interstitial cells of Cajal is fundamental for slow wave activity in gastrointestinal muscles.. J. Physiol. 2009;587, 4887-4904.
  27. Gomez-Pinilla PJ, Gibbons SJ, Bardsley MR, Lorincz A, Pozo MJ, Pasricha PJ, Van de Rijn M, West RB, Sarr MG, Kendrick ML, Cima RR, Dozois EJ, Larson DW, Ordog T, Farrugia G. Ano1 is a selective marker of interstitial cells of Cajal in the human and mouse gastrointestinal tract.. Am. J. Physiol. Gastrointest. Liver Physiol. 2009;296, G1370-1381.
  28. O’Driscoll KE, Pipe RA, Britton FC. Increased complexity of Tmem16a/Anoctamin 1 transcript alternative splicing.. BMC Mol. Biol. 2011;12, 35.
  29. Wang XY, Chen JH, Li K, Zhu YF, Wright GW, Huizinga JD. Discrepancies between c-Kit positive and Ano1 positive ICC-SMP in the W/Wv and wild-type mouse colon; relationships with motor patterns and calcium transients.. Neurogastroenterol. Motil. 2014;26, 1298-1310.
  30. Chen H, Ordög T, Chen J, Young DL, Bardsley MR, Redelman D, Ward SM, Sanders KM. Differential gene expression in functional classes of interstitial cells of Cajal in murine small intestine.. Physiol. Genomics 2007;31, 492-509.
  31. Hudson NP, Pearson GT, Kitamura N, Mayhew IG. An immunohistochemical study of interstitial cells of Cajal (ICC) in the equine gastrointestinal tract.. Res. Vet. Sci. 1999;66, 265-271.
  32. Hudson N, Mayhew I, Pearson G. A reduction in interstitial cells of Cajal in horses with equine dysautonomia (grass sickness).. Auton. Neurosci. 2001;92, 37-44.
  33. Pavone S, Mandara MT. A morphological and quantitative immunohistochemical study of the interstitial cells of Cajal in the normal equine intestinal tracts.. Equine Vet. J. 2010;42, 358-366.
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