FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Vet Immunol Immunopathol / Myristoylated Alanine Rich C Kinase Substrate (MARCKS) is es...
Veterinary immunology and immunopathology2014; 160(3-4); 167-176; doi: 10.1016/j.vetimm.2014.04.009

Myristoylated Alanine Rich C Kinase Substrate (MARCKS) is essential to β2-integrin dependent responses of equine neutrophils.

Abstract: Neutrophil infiltration is a prominent feature in a number of pathologic conditions affecting horses including recurrent airway obstruction, ischemia-reperfusion injury, and laminitis. Cell signaling components involved in neutrophil migration represent targets for novel anti-inflammatory therapies. In order to migrate into tissue, neutrophils must respond to chemoattractant signals in their external environment through activation of adhesion receptors (i.e. integrins) and reorganization of the actin cytoskeleton. Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS), a highly conserved actin-binding protein, has a well demonstrated role in cytoskeletal dependent cellular functions (i.e. adhesion, spreading, and migration), but the details of MARCKS involvement in these processes remain vague. We hypothesized that MARCKS serves as a link between the actin cytoskeleton and integrin function in neutrophils. Using a MARCKS-specific inhibitor peptide known as MANS on equine neutrophils in vitro, we demonstrate that inhibition of MARCKS function significantly attenuates β2-integrin-dependent neutrophil functions including migration, adhesion, and immune complex-mediated respiratory burst. The MANS peptide did not, however, inhibit the β2-integrin-independent PMA mediated respiratory burst. These results attest to the essential role of MARCKS function in regulating neutrophil responses, and strongly implicate MARCKS as a potential regulator of β2-integrins in neutrophils.
Copyright © 2014 Elsevier B.V. All rights reserved.
Get Full Text
Publication Date: 2014-05-02 PubMed ID: 24857637PubMed Central: PMC4108539DOI: 10.1016/j.vetimm.2014.04.009Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article
  • Research Support
  • N.I.H.
  • Extramural
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research explores the key role of the protein MARCKS (Myristoylated Alanine-Rich C-Kinase Substrate) in the functioning of neutrophils—namely, migration, adhesion, and immune complex-related respiratory burst—in horses’ bodies. The findings suggest that its inhibition affects β2-integrin-dependent functionalities, highlighting MARCKS as a probable regulator of β2-integrins in neutrophils.

Importance of Neutrophils and The Role of MARCKS

  • The research underlines the role of neutrophils, a type of white blood cell that is typically the first to respond to inflammation, in several pathologic conditions affecting horses, like recurrent airway obstruction, ischemia-reperfusion injury, and laminitis.
  • Understanding the signaling components involved in the migration of neutrophils is crucial to forming potential anti-inflammatory treatments.
  • The protein MARCKS is significant because it plays a big role in cytoskeleton-dependent cellular functions such as adhesion, spreading, and migration. However, its precise involvement in these processes hasn’t been clearly defined.
  • The researchers posit that MARCKS could function as a connecting link between the actin cytoskeleton and integrin function in neutrophils.

Effect of Inhibiting MARCKS Function

  • For the study, researchers used a MARCKS-specific inhibiting agent (the MANS peptide) on equine neutrophils in a lab setting.
  • The results showed that when MARCKS function was inhibited, there was significant reduction in β2-integrin-dependent neutrophil functionalities, such as migration, adhesion, and immune complex-related respiratory burst.
  • The MANS peptide did not, however, inhibit the β2-integrin-independent PMA mediated respiratory burst, shedding light on the specific and critical role of MARCKS in regulating β2-integrin-related neutrophil responses.

Implications of the Research

  • These findings highlight the vital role of MARCKS in regulating neutrophil responses and suggest that it may serve as a potential regulator of β2-integrins in neutrophils.
  • The research paves the way for further exploration into the role of MARCKS and how it can be used to develop novel anti-inflammatory therapies for conditions involving neutrophil infiltration in horses.

Cite This Article

APA
Sheats MK, Pescosolido KC, Hefner EM, Sung EJ, Adler KB, Jones SL. (2014). Myristoylated Alanine Rich C Kinase Substrate (MARCKS) is essential to β2-integrin dependent responses of equine neutrophils. Vet Immunol Immunopathol, 160(3-4), 167-176. https://doi.org/10.1016/j.vetimm.2014.04.009

Publication

Veterinary immunology and immunopathology
ISSN: 1873-2534
NlmUniqueID: 8002006
Country: Netherlands
Language: English
Volume: 160
Issue: 3-4
Pages: 167-176
PII: S0165-2427(14)00112-3

Researcher Affiliations

Sheats, Mary K
  • Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, United States; Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, United States. Electronic address: mkpeed@ncsu.edu.
Pescosolido, Kimberly C
  • Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, United States.
Hefner, Ethan M
  • Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, United States.
Sung, Eui Jae
  • Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, United States.
Adler, Kenneth B
  • Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, United States.
Jones, Samuel L
  • Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, United States; Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, United States.

MeSH Terms

  • Amino Acid Sequence
  • Animals
  • Antigen-Antibody Complex / physiology
  • CD18 Antigens / immunology
  • CD18 Antigens / physiology
  • Cell Adhesion / drug effects
  • Cell Adhesion / physiology
  • Cell Movement / drug effects
  • Cell Movement / physiology
  • Conserved Sequence
  • Horses / immunology
  • Horses / physiology
  • In Vitro Techniques
  • Intracellular Signaling Peptides and Proteins / antagonists & inhibitors
  • Intracellular Signaling Peptides and Proteins / genetics
  • Intracellular Signaling Peptides and Proteins / physiology
  • Membrane Proteins / antagonists & inhibitors
  • Membrane Proteins / genetics
  • Membrane Proteins / physiology
  • Molecular Sequence Data
  • Myristoylated Alanine-Rich C Kinase Substrate
  • Neutrophil Infiltration / drug effects
  • Neutrophil Infiltration / immunology
  • Neutrophil Infiltration / physiology
  • Peptide Fragments / genetics
  • Peptide Fragments / pharmacology
  • Respiratory Burst / drug effects
  • Sequence Homology, Amino Acid
  • Tetradecanoylphorbol Acetate / pharmacology

Grant Funding

  • R37 HL036982 / NHLBI NIH HHS
  • T32 RR024394 / NCRR NIH HHS
  • K01 OD015136 / NIH HHS
  • R37 HL36982 / NHLBI NIH HHS
  • T32 OD011130 / NIH HHS
  • 5K01OD015136 / NIH HHS
  • T35 OD011070 / NIH HHS
  • T35 RR025837 / NCRR NIH HHS

Conflict of Interest Statement

. KBA holds 150,000 founders shares of a start-up biotech company, BioMarck, and serves as a scientific consultant and member of the scientific advisory board without monetary compensation. KBA receives over $100,000 yearly in research grants from National Institutes of Health (NIH) and U.S. Environmental Protection Agency. KBA is Editor-in-Chief of the American Journal of Respiratory Cell and Molecular Biology and receives a stipend of <$100,000 per year from the American Thoracic Society for this. The remaining authors have declared that no competing interests exist.

References

This article includes 47 references
  1. Aderem A. The MARCKS brothers: a family of protein kinase C substrates.. Cell 1992 Nov 27;71(5):713-6.
    pubmed: 1423627doi: 10.1016/0092-8674(92)90546-ogoogle scholar: lookup
  2. Arbuzova A, Schmitz AA, Vergères G. Cross-talk unfolded: MARCKS proteins.. Biochem J 2002 Feb 15;362(Pt 1):1-12.
    pmc: PMC1222354pubmed: 11829734doi: 10.1042/0264-6021:3620001google scholar: lookup
  3. Baggiolini M. Chemokines and leukocyte traffic.. Nature 1998 Apr 9;392(6676):565-8.
    pubmed: 9560152doi: 10.1038/33340google scholar: lookup
  4. Berger C, Rossaint J, Van Aken H, Westphal M, Hahnenkamp K, Zarbock A. Lidocaine reduces neutrophil recruitment by abolishing chemokine-induced arrest and transendothelial migration in septic patients.. J Immunol 2014 Jan 1;192(1):367-76.
    pubmed: 24293632doi: 10.4049/jimmunol.1301363google scholar: lookup
  5. Blackshear PJ. The MARCKS family of cellular protein kinase C substrates.. J Biol Chem 1993 Jan 25;268(3):1501-4.
    pubmed: 8420923
  6. Cazzola M, Page CP, Calzetta L, Matera MG. Emerging anti-inflammatory strategies for COPD.. Eur Respir J 2012 Sep;40(3):724-41.
    pubmed: 22496331doi: 10.1183/09031936.00213711google scholar: lookup
  7. Cicchetti G, Allen PG, Glogauer M. Chemotactic signaling pathways in neutrophils: from receptor to actin assembly.. Crit Rev Oral Biol Med 2002;13(3):220-8.
    pubmed: 12090462doi: 10.1177/154411130201300302google scholar: lookup
  8. Clark EA, Brugge JS. Integrins and signal transduction pathways: the road taken.. Science 1995 Apr 14;268(5208):233-9.
    pubmed: 7716514doi: 10.1126/science.7716514google scholar: lookup
  9. Colditz IG. Margination and emigration of leucocytes.. Surv Synth Pathol Res 1985;4(1):44-68.
    pubmed: 3895348doi: 10.1159/000156964google scholar: lookup
  10. de la Rebière de Pouyade G, Serteyn D. The role of activated neutrophils in the early stage of equine laminitis.. Vet J 2011 Jul;189(1):27-33.
    pubmed: 20655252doi: 10.1016/j.tvjl.2010.06.008google scholar: lookup
  11. Dillon SB, Verghese MW, Snyderman R. Signal transduction in cells following binding of chemoattractants to membrane receptors.. Virchows Arch B Cell Pathol Incl Mol Pathol 1988;55(2):65-80.
    pubmed: 2901161doi: 10.1007/BF02896561google scholar: lookup
  12. Disatnik MH, Boutet SC, Pacio W, Chan AY, Ross LB, Lee CH, Rando TA. The bi-directional translocation of MARCKS between membrane and cytosol regulates integrin-mediated muscle cell spreading.. J Cell Sci 2004 Sep 1;117(Pt 19):4469-79.
    pubmed: 15316066doi: 10.1242/jcs.01309google scholar: lookup
  13. Eckert RE, Neuder LE, Park J, Adler KB, Jones SL. Myristoylated alanine-rich C-kinase substrate (MARCKS) protein regulation of human neutrophil migration.. Am J Respir Cell Mol Biol 2010 May;42(5):586-94.
    pmc: PMC2874444pubmed: 19574534doi: 10.1165/rcmb.2008-0394OCgoogle scholar: lookup
  14. Foxman EF, Campbell JJ, Butcher EC. Multistep navigation and the combinatorial control of leukocyte chemotaxis.. J Cell Biol 1997 Dec 1;139(5):1349-60.
    pmc: PMC2140208pubmed: 9382879doi: 10.1083/jcb.139.5.1349google scholar: lookup
  15. Frevert CW, Wong VA, Goodman RB, Goodwin R, Martin TR. Rapid fluorescence-based measurement of neutrophil migration in vitro.. J Immunol Methods 1998 Apr 1;213(1):41-52.
    pubmed: 9671124doi: 10.1016/s0022-1759(98)00016-7google scholar: lookup
  16. Fujishima S, Aikawa N. Neutrophil-mediated tissue injury and its modulation.. Intensive Care Med 1995 Mar;21(3):277-85.
    pubmed: 7790621doi: 10.1007/BF01701489google scholar: lookup
  17. Gerard MP, Blikslager AT, Roberts MC, Tate LP Jr, Argenzio RA. The characteristics of intestinal injury peripheral to strangulating obstruction lesions in the equine small intestine.. Equine Vet J 1999 Jul;31(4):331-5.
    pubmed: 10454093doi: 10.1111/j.2042-3306.1999.tb03826.xgoogle scholar: lookup
  18. Hartwig JH, Thelen M, Rosen A, Janmey PA, Nairn AC, Aderem A. MARCKS is an actin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin.. Nature 1992 Apr 16;356(6370):618-22.
    pubmed: 1560845doi: 10.1038/356618a0google scholar: lookup
  19. Hurley DJ, Parks RJ, Reber AJ, Donovan DC, Okinaga T, Vandenplas ML, Peroni JF, Moore JN. Dynamic changes in circulating leukocytes during the induction of equine laminitis with black walnut extract.. Vet Immunol Immunopathol 2006 Apr 15;110(3-4):195-206.
    pubmed: 16290066doi: 10.1016/j.vetimm.2005.09.015google scholar: lookup
  20. Hynes RO. Integrins: versatility, modulation, and signaling in cell adhesion.. Cell 1992 Apr 3;69(1):11-25.
    pubmed: 1555235doi: 10.1016/0092-8674(92)90115-sgoogle scholar: lookup
  21. Jones SL, Knaus UG, Bokoch GM, Brown EJ. Two signaling mechanisms for activation of alphaM beta2 avidity in polymorphonuclear neutrophils.. J Biol Chem 1998 Apr 24;273(17):10556-66.
    pubmed: 9553116doi: 10.1074/jbc.273.17.10556google scholar: lookup
  22. Jones SL, Sharief Y, Chilcoat CD. Signaling mechanism for equine neutrophil activation by immune complexes.. Vet Immunol Immunopathol 2001 Sep 28;82(1-2):87-100.
    pubmed: 11557296doi: 10.1016/s0165-2427(01)00350-6google scholar: lookup
  23. Kraan MC, de Koster BM, Elferink JG, Post WJ, Breedveld FC, Tak PP. Inhibition of neutrophil migration soon after initiation of treatment with leflunomide or methotrexate in patients with rheumatoid arthritis: findings in a prospective, randomized, double-blind clinical trial in fifteen patients.. Arthritis Rheum 2000 Jul;43(7):1488-95.
    pubmed: 10902750doi: 10.1002/1529-0131(200007)43:7<1488::AID-ANR11>3.0.CO;2-Ggoogle scholar: lookup
  24. Kurtel H, Tso P, Granger DN. Granulocyte accumulation in postischemic intestine: role of leukocyte adhesion glycoprotein CD11/CD18.. Am J Physiol 1992 May;262(5 Pt 1):G878-82.
    pubmed: 1350422doi: 10.1152/ajpgi.1992.262.5.G878google scholar: lookup
  25. Larsson C. Protein kinase C and the regulation of the actin cytoskeleton.. Cell Signal 2006 Mar;18(3):276-84.
    pubmed: 16109477doi: 10.1016/j.cellsig.2005.07.010google scholar: lookup
  26. Lehmeyer JE, Snyderman R, Johnston RB Jr. Stimulation of neutrophil oxidative metabolism by chemotactic peptides: influence of calcium ion concentration and cytochalasin B and comparison with stimulation by phorbol myristate acetate.. Blood 1979 Jul;54(1):35-45.
    pubmed: 444673
  27. Ley K, Laudanna C, Cybulsky MI, Nourshargh S. Getting to the site of inflammation: the leukocyte adhesion cascade updated.. Nat Rev Immunol 2007 Sep;7(9):678-89.
    pubmed: 17717539doi: 10.1038/nri2156google scholar: lookup
  28. Li J, D'Annibale-Tolhurst MA, Adler KB, Fang S, Yin Q, Birkenheuer AJ, Levy MG, Jones SL, Sung EJ, Hawkins EC, Yoder JA, Nordone SK. A myristoylated alanine-rich C kinase substrate-related peptide suppresses cytokine mRNA and protein expression in LPS-activated canine neutrophils.. Am J Respir Cell Mol Biol 2013 Mar;48(3):314-21.
    pmc: PMC3604091pubmed: 23221047doi: 10.1165/rcmb.2012-0278OCgoogle scholar: lookup
  29. Little D, Tomlinson JE, Blikslager AT. Post operative neutrophilic inflammation in equine small intestine after manipulation and ischaemia.. Equine Vet J 2005 Jul;37(4):329-35.
    pubmed: 16028622doi: 10.2746/0425164054529472google scholar: lookup
  30. Luo BH, Carman CV, Springer TA. Structural basis of integrin regulation and signaling.. Annu Rev Immunol 2007;25:619-47.
    pmc: PMC1952532pubmed: 17201681doi: 10.1146/annurev.immunol.25.022106.141618google scholar: lookup
  31. Marinkovic D, Aleksic-Kovacevic S, Plamenac P. Cellular basis of chronic obstructive pulmonary disease in horses.. Int Rev Cytol 2007;257:213-47.
    pubmed: 17280899doi: 10.1016/S0074-7696(07)57006-3google scholar: lookup
  32. Moore RM, Muir WW, Granger DN. Mechanisms of gastrointestinal ischemia-reperfusion injury and potential therapeutic interventions: a review and its implications in the horse.. J Vet Intern Med 1995 May-Jun;9(3):115-32.
    pubmed: 7674213doi: 10.1111/j.1939-1676.1995.tb03285.xgoogle scholar: lookup
  33. Nauseef WM. Isolation of human neutrophils from venous blood.. Methods Mol Biol 2007;412:15-20.
    pubmed: 18453102doi: 10.1007/978-1-59745-467-4_2google scholar: lookup
  34. Nishida N, Xie C, Shimaoka M, Cheng Y, Walz T, Springer TA. Activation of leukocyte beta2 integrins by conversion from bent to extended conformations.. Immunity 2006 Oct;25(4):583-94.
    pubmed: 17045822doi: 10.1016/j.immuni.2006.07.016google scholar: lookup
  35. Ott LE, Sung EJ, Melvin AT, Sheats MK, Haugh JM, Adler KB, Jones SL. Fibroblast Migration Is Regulated by Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) Protein.. PLoS One 2013;8(6):e66512.
    pmc: PMC3686679pubmed: 23840497doi: 10.1371/journal.pone.0066512google scholar: lookup
  36. Rosales C, Juliano RL. Signal transduction by cell adhesion receptors in leukocytes.. J Leukoc Biol 1995 Feb;57(2):189-98.
    pubmed: 7852832doi: 10.1002/jlb.57.2.189google scholar: lookup
  37. Schmidt S, Moser M, Sperandio M. The molecular basis of leukocyte recruitment and its deficiencies.. Mol Immunol 2013 Aug;55(1):49-58.
    pubmed: 23253941doi: 10.1016/j.molimm.2012.11.006google scholar: lookup
  38. Schymeinsky J, Mócsai A, Walzog B. Neutrophil activation via beta2 integrins (CD11/CD18): molecular mechanisms and clinical implications.. Thromb Haemost 2007 Aug;98(2):262-73.
    pubmed: 17721605
  39. Singer M, Martin LD, Vargaftig BB, Park J, Gruber AD, Li Y, Adler KB. A MARCKS-related peptide blocks mucus hypersecretion in a mouse model of asthma.. Nat Med 2004 Feb;10(2):193-6.
    pubmed: 14716307doi: 10.1038/nm983google scholar: lookup
  40. Sisková Z, Baron W, de Vries H, Hoekstra D. Fibronectin impedes "myelin" sheet-directed flow in oligodendrocytes: a role for a beta 1 integrin-mediated PKC signaling pathway in vesicular trafficking.. Mol Cell Neurosci 2006 Oct;33(2):150-9.
    pubmed: 16935002doi: 10.1016/j.mcn.2006.07.001google scholar: lookup
  41. Springer TA. Adhesion receptors of the immune system.. Nature 1990 Aug 2;346(6283):425-34.
    pubmed: 1974032doi: 10.1038/346425a0google scholar: lookup
  42. Takashi S, Park J, Fang S, Koyama S, Parikh I, Adler KB. A peptide against the N-terminus of myristoylated alanine-rich C kinase substrate inhibits degranulation of human leukocytes in vitro.. Am J Respir Cell Mol Biol 2006 Jun;34(6):647-52.
    pmc: PMC2644225pubmed: 16543603doi: 10.1165/rcmb.2006-0030RCgoogle scholar: lookup
  43. Thelen M, Rosen A, Nairn AC, Aderem A. Regulation by phosphorylation of reversible association of a myristoylated protein kinase C substrate with the plasma membrane.. Nature 1991 May 23;351(6324):320-2.
    pubmed: 2034276doi: 10.1038/351320a0google scholar: lookup
  44. Uriarte SM, Rane MJ, Merchant ML, Jin S, Lentsch AB, Ward RA, McLeish KR. Inhibition of neutrophil exocytosis ameliorates acute lung injury in rats.. Shock 2013 Mar;39(3):286-92.
    pmc: PMC3578018pubmed: 23364427doi: 10.1097/SHK.0b013e318282c9a1google scholar: lookup
  45. Williams MA, Solomkin JS. Integrin-mediated signaling in human neutrophil functioning.. J Leukoc Biol 1999 Jun;65(6):725-36.
    pubmed: 10380892doi: 10.1002/jlb.65.6.725google scholar: lookup
  46. Wong DM, Moore RM, Brockus CW. Mechanisms of oxidative injury in equine disease.. Compend Contin Educ Vet 2012 Aug;34(8):E6.
    pubmed: 22935994
  47. Zhou X, Li J. Macrophage-enriched myristoylated alanine-rich C kinase substrate and its phosphorylation is required for the phorbol ester-stimulated diffusion of beta 2 integrin molecules.. J Biol Chem 2000 Jun 30;275(26):20217-22.
    pubmed: 10779523doi: 10.1074/jbc.M909129199google scholar: lookup

Citations

This article has been cited 13 times.
  1. Conley H, Till RL, Berglund AK, Jones SL, Sheats MK. A myristoylated alanine-rich C-kinase substrate (MARCKS) inhibitor peptide attenuates neutrophil outside-in β(2)-integrin activation and signaling.. Cell Adh Migr 2023 Dec;17(1):1-16.
    doi: 10.1080/19336918.2023.2233204pubmed: 37439125google scholar: lookup
  2. Conley HE, Sheats MK. Targeting Neutrophil β(2)-Integrins: A Review of Relevant Resources, Tools, and Methods.. Biomolecules 2023 May 26;13(6).
    doi: 10.3390/biom13060892pubmed: 37371473google scholar: lookup
  3. Bayless RL, Sheats MK, Jones SL. Withaferin A Inhibits Neutrophil Adhesion, Migration, and Respiratory Burst and Promotes Timely Neutrophil Apoptosis.. Front Vet Sci 2022;9:900453.
    doi: 10.3389/fvets.2022.900453pubmed: 35782542google scholar: lookup
  4. Stonex T, Salmon JH, Adler KB, Gilger BC. Peptide Inhibitors of MARCKS Suppress Endotoxin Induced Uveitis in Rats.. J Ocul Pharmacol Ther 2022 Apr;38(3):223-231.
    doi: 10.1089/jop.2021.0114pubmed: 35385320google scholar: lookup
  5. Gaurav R, Mikuls TR, Thiele GM, Nelson AJ, Niu M, Guda C, Eudy JD, Barry AE, Wyatt TA, Romberger DJ, Duryee MJ, England BR, Poole JA. High-throughput analysis of lung immune cells in a combined murine model of agriculture dust-triggered airway inflammation with rheumatoid arthritis.. PLoS One 2021;16(2):e0240707.
    doi: 10.1371/journal.pone.0240707pubmed: 33577605google scholar: lookup
  6. Sheats MK, Yin Q, Fang S, Park J, Crews AL, Parikh I, Dickson B, Adler KB. MARCKS and Lung Disease.. Am J Respir Cell Mol Biol 2019 Jan;60(1):16-27.
    doi: 10.1165/rcmb.2018-0285TRpubmed: 30339463google scholar: lookup
  7. Westerman TL, Bogomolnaya L, Andrews-Polymenis HL, Sheats MK, Elfenbein JR. The Salmonella type-3 secretion system-1 and flagellar motility influence the neutrophil respiratory burst.. PLoS One 2018;13(9):e0203698.
    doi: 10.1371/journal.pone.0203698pubmed: 30204776google scholar: lookup
  8. El Amri M, Fitzgerald U, Schlosser G. MARCKS and MARCKS-like proteins in development and regeneration.. J Biomed Sci 2018 May 22;25(1):43.
    doi: 10.1186/s12929-018-0445-1pubmed: 29788979google scholar: lookup
  9. Brudvig JJ, Cain JT, Schmidt-Grimminger GG, Stumpo DJ, Roux KJ, Blackshear PJ, Weimer JM. MARCKS Is Necessary for Netrin-DCC Signaling and Corpus Callosum Formation.. Mol Neurobiol 2018 Nov;55(11):8388-8402.
    doi: 10.1007/s12035-018-0990-3pubmed: 29546593google scholar: lookup
  10. Martin EM, Till RL, Sheats MK, Jones SL. Misoprostol Inhibits Equine Neutrophil Adhesion, Migration, and Respiratory Burst in an In Vitro Model of Inflammation.. Front Vet Sci 2017;4:159.
    doi: 10.3389/fvets.2017.00159pubmed: 29034248google scholar: lookup
  11. Yin Q, Fang S, Park J, Crews AL, Parikh I, Adler KB. An Inhaled Inhibitor of Myristoylated Alanine-Rich C Kinase Substrate Reverses LPS-Induced Acute Lung Injury in Mice.. Am J Respir Cell Mol Biol 2016 Nov;55(5):617-622.
    doi: 10.1165/rcmb.2016-0236RCpubmed: 27556883google scholar: lookup
  12. Birse KD, Cole AL, Hirbod T, McKinnon L, Ball TB, Westmacott GR, Kimani J, Plummer F, Cole AM, Burgener A, Broliden K. Non-Cationic Proteins Are Associated with HIV Neutralizing Activity in Genital Secretions of Female Sex Workers.. PLoS One 2015;10(6):e0130404.
    doi: 10.1371/journal.pone.0130404pubmed: 26090884google scholar: lookup
  13. Sheats MK, Sung EJ, Adler KB, Jones SL. In Vitro Neutrophil Migration Requires Protein Kinase C-Delta (δ-PKC)-Mediated Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) Phosphorylation.. Inflammation 2015;38(3):1126-41.
    doi: 10.1007/s10753-014-0078-9pubmed: 25515270google scholar: lookup
Subscribe to our newsletter
Join 99,359 horse owners like you who receive our email updates on horse health and nutrition.