Natural Horse Boarding Vs Traditional Stable: A Comparison of Hormonal, Hematological and Immunological Parameters.
Abstract: In the equestrian world, two different types of management can be distinguished: traditional management and natural boarding. The aim of this research was to compare hormonal, hematological and immunological parameters of 47 horses kept in these two different managements. Blood and horsehair of the horses were sampled to determine DHEA (dehydroepiandrosterone) and cortisol concentration through RIA. Moreover, blood count was conducted, and flow cytometry was employed to phenotype lymphocyte subpopulations. Results showed that, in horsehair, DHEA concentration was significantly higher in natural horses, whereas cortisol concentration and cortisol/DEHA ratio significantly lower. These hormonal parameters are used to assess the stress condition and the welfare of animals. The most favorable endocrine framework found in horses kept in natural boarding suggests that this management conveys most with ethological and physiological needs of the species. The research underlines the need of a modification of horses' husbandry systems. For the first time, this study validates the assay of DHEA in horsehair.
Publication Date: 2019-09-05 PubMed ID: 32441218DOI: 10.1080/10888705.2019.1663737Google Scholar: Lookup
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- Comparative Study
- Journal Article
Summary
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This research study compares the impacts of traditional horse stabling and natural horse boarding on hormonal, hematological, and immunological parameters in horses. The findings suggest that horses in natural boarding conditions have lower stress levels and improved welfare, pointing to the need for changes in horse management practices.
Introduction to the Study
- The study focuses on two distinct methods of horse management: traditional stabling which often involves restricted movement and individual isolation, and natural boarding which employs a more free-roaming and social environment for the horses.
- The researchers aimed to draw a comparison by measuring and observing the hormonal, hematological, and immunological changes in horses housed under these different conditions.
- They sampled blood and horsehair from 47 horses for these comparisons.
Methods Used in the Study
- Blood and horsehair samples were collected from 47 horses.
- The researchers evaluated the concentrations of two hormones (DHEA, or dehydroepiandrosterone, and cortisol) via radioimmunoassay (RIA) to measure stress levels in the horses.
- These hormonal parameters are commonly used indicators of the animals’ stress conditions and overall welfare.
- Blood count was conducted to provide a general picture of the horses’ health status.
- Lymphocyte subpopulations were identified using a method called flow cytometry, which is used to investigate the immune system of these animals.
Key Findings of the Study
- The study found a significantly higher concentration of DHEA and significantly lower levels of cortisol and cortisol/DHEA ratio in the horsehair of horses kept in natural boarding conditions.
- This indicates a lower stress level and overall better welfare in hoses kept under natural boarding versus those in a traditional stable.
- The results suggest that the natural boarding style is more in line with the ethological (behavioral) and physiological needs of horses.
- Additionally, the study conclusively demonstrated, for the first time, that measuring DHEA in horse hair is a valid testing methodology.
Implications and Conclusion
- The study calls for a reevaluation of traditional horse husbandry systems, given the evidence of the more positive outcomes associated with the practice of natural horse boarding.
- Through its findings, the research suggests that the welfare of horses can be elevated by modifying current management systems to more naturally align with their instinctive behavioural and physiological needs.
Cite This Article
APA
Placci M, Marliani G, Sabioni S, Gabai G, Mondo E, Borghetti P, De Angelis E, Accorsi PA.
(2019).
Natural Horse Boarding Vs Traditional Stable: A Comparison of Hormonal, Hematological and Immunological Parameters.
J Appl Anim Welf Sci, 23(3), 366-377.
https://doi.org/10.1080/10888705.2019.1663737 Publication
Researcher Affiliations
- Dipartimento di Scienze Mediche Veterinarie, University of Bologna , Ozzano Emilia (BO), Italy.
- Dipartimento di Scienze Mediche Veterinarie, University of Bologna , Ozzano Emilia (BO), Italy.
- Veterinario libero professionista , Castel del Rio (BO), Italy.
- Dipartimento di Scienze Sperimentali Veterinarie, University of Padova , Legnaro (PD), Italy.
- Dipartimento di Scienze Mediche Veterinarie, University of Bologna , Ozzano Emilia (BO), Italy.
- Department of Animal Health, University of Parma , Parma (PR), Italy.
- Department of Animal Health, University of Parma , Parma (PR), Italy.
- Dipartimento di Scienze Mediche Veterinarie, University of Bologna , Ozzano Emilia (BO), Italy.
MeSH Terms
- Animal Husbandry
- Animal Welfare
- Animals
- Dehydroepiandrosterone / analysis
- Female
- Hair / chemistry
- Hormones
- Horses / blood
- Horses / immunology
- Horses / physiology
- Housing, Animal
- Hydrocortisone / analysis
- Male
- Stress, Physiological
Citations
This article has been cited 12 times.- Torres Borda L, Auer U, Jenner F. Equine Social Behaviour: Love, War and Tolerance. Animals (Basel) 2023 Apr 26;13(9).
- Kieson E, Goma AA, Radi M. Tend and Befriend in Horses: Partner Preferences, Lateralization, and Contextualization of Allogrooming in Two Socially Stable Herds of Quarter Horse Mares. Animals (Basel) 2023 Jan 7;13(2).
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- Lanci A, Mariella J, Ellero N, Faoro A, Peric T, Prandi A, Freccero F, Castagnetti C. Hair Cortisol and DHEA-S in Foals and Mares as a Retrospective Picture of Feto-Maternal Relationship under Physiological and Pathological Conditions. Animals (Basel) 2022 May 14;12(10).
- Hildebrandt F, Büttner K, Salau J, Krieter J, Czycholl I. Area and Resource Utilization of Group-Housed Horses in an Active Stable. Animals (Basel) 2021 Sep 23;11(10).
- Olvera-Maneu S, Carbajal A, Gardela J, Lopez-Bejar M. Hair Cortisol, Testosterone, Dehydroepiandrosterone Sulfate and Their Ratios in Stallions as a Retrospective Measure of Hypothalamic-Pituitary-Adrenal and Hypothalamic-Pituitary-Gonadal Axes Activity: Exploring the Influence of Seasonality. Animals (Basel) 2021 Jul 25;11(8).
- Whitham JC, Bryant JL, Miller LJ. Beyond Glucocorticoids: Integrating Dehydroepiandrosterone (DHEA) into Animal Welfare Research. Animals (Basel) 2020 Aug 9;10(8).
- Arbogast DM, Metrione LC, Jones MK, Donelan EM, Roth TL, Freeman EW, Rispoli LA. Pregnancy- and age-associated variation in serum dehydroepiandrosterone concentrations in black and white rhinoceroses. Conserv Physiol 2026;14(1):coag007.
- Castellani F, Ricci M, Rosato R, Manucci A, Di Simone V, Cerasoli F, Calandrini A, Costa ED, Vulpiani MP, Scortichini G, Saluti G. Surrogate analyte approach for the determination of endogenous cortisol, cortisone, DHEA, DHEAS in horse hair and sheep wool by LC-HRMS/MS. Sci Rep 2025 Apr 29;15(1):14987.
- Robertson T, Thomas E, Starbuck G, Yarnell K. Global distribution and gap analysis of equine housing research: The findings so far and where to go next. Anim Welf 2024;33:e58.
- Bruschetta G, Zanghì G, Giunta RP, Ferlazzo AM, Satué K, D'Ascola A, Fazio E. Short Road Transport and Slaughter Stress Affects the Expression Profile of Serotonin Receptors, Adrenocortical, and Hematochemical Responses in Horses. Vet Sci 2024 Mar 3;11(3).
- Interlandi C, Tabbì M, Di Pietro S, D'Angelo F, Costa GL, Arfuso F, Giudice E, Licata P, Macrì D, Crupi R, Gugliandolo E. Improved quality of life and pain relief in mature horses with osteoarthritis after oral transmucosal cannabidiol oil administration as part of an analgesic regimen. Front Vet Sci 2024;11:1341396.
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