Nature of the heterogenetic hapten reacting with hemagglutinins in horse serum sickness.
Abstract: 1. From beef stroma pretreated at room temperature with acetone and alcohol, a fraction was isolated with boiling 100% alcohol which, in a dilution of 1:500,000, combines with the sheep blood agglutinin of human serum produced during serum sickness. Using the terminology of Schiff, this fraction corresponds to the heterogenetic serum sickness antigen; it could be separated to a large extent from the heterogenetic mononucleosis antigen which also occurs in beef stroma.
2. The so-called serum sickness antigen also occurs heterogenetically in guinea pig kidney and it could also be isolated from the Forrsman antigen although without increase in activity.
3. Horse serum is a poorer source for isolation of The so-called serum sickness antigen. The latter could be isolated from the serum, after removal of the albumin bodies by coagulation with heat, with an activity of 1:1,500.
Publication Date: 1948-12-01 PubMed ID: 18122856DOI: 10.3181/00379727-69-16787Google Scholar: Lookup
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- Journal Article
Summary
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The research focuses on isolating a particular component, known as the heterogenetic serum sickness antigen, from beef stroma. This component reacts with sheep blood agglutinins, proteins produced by the human immune system during serum sickness. Also, this antigen could be found in a certain part of guinea pig kidneys and can be isolated from Forrsman antigen. However, horse serum was found to be a less suitable source for isolating this antigen.
Heterogenetic Serum Sickness Antigen
- The researchers began by pretreating beef stroma with acetone and alcohol at room temperature to isolate a fraction with boiling 100% alcohol. This fraction, diluted to 1:500,000, reacts with the sheep blood agglutinin, an element of human serum produced as a response during serum sickness. According to Schiff’s terminology, this fraction corresponds to the heterogenetic serum sickness antigen. It could be differentiated, largely, from the heterogenetic mononucleosis antigen which also occurs in beef stroma.
Alternative Sources of the Antigen
- The research found that the heterogenetic serum sickness antigen is not only present in beef stroma but can also be found in guinea pig kidneys. The scientists were able to isolate the antigen from the Forrsman antigen without increasing its activity. This finding broadens the possible sources for this antigen which can prove valuable in further studies.
Horse Serum as a Source for the Antigen
- The research explored the possibility of horse serum being a source for the heterogenetic serum sickness antigen. The yielded results were less promising as the isolation process proved to be more challenging. The activity of the isolated antigen from horse serum was 1:1,500, quite lower than that obtained from beef stroma. Consequently, the albumin bodies needed to be removed by heat coagulation before the antigen could be isolated. Hence, the horse serum is deemed a poorer source for the isolation of this antigen.
Cite This Article
APA
TOMCSIK J, SCHWARZWEISS H.
(1948).
Nature of the heterogenetic hapten reacting with hemagglutinins in horse serum sickness.
Proc Soc Exp Biol Med, 69(3), 562-565.
https://doi.org/10.3181/00379727-69-16787 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Haptens
- Hemagglutinins
- Horses
- Immunologic Factors
- Serum Sickness
Citations
This article has been cited 4 times.- TOMCSIK J, SCHILD CA. [Injury and spherical modifications of erythrocytes in hemagglutination]. Experientia 1950 Nov 15;6(11):422-3.
- MULLER F. [Complement fixation antibodies in infectious mononucleosis (Pfeiffer's glandular fever); preliminary report]. Klin Wochenschr 1958 Jul 15;36(14):685-6.
- CARTER BB. Studies of lipid extracts from red blood cells. Experientia 1957 Sep 15;13(9):367-8.
- LIPPELT H, NOGALSKI J. [Heterochemagglutination]. Z Hyg Infektionskr 1953;136(2):177-99.
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