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Cells2019; 8(12); doi: 10.3390/cells8121528

Neutrophil Extracellular Traps in the Pathogenesis of Equine Recurrent Uveitis (ERU).

Abstract: Equine recurrent uveitis (ERU) is considered one of the most important eye diseases in horses and typically appears with relapsing inflammatory episodes without systemic effects. Various disorders have been described as an initial trigger, including infections. Independent of the initiating cause, there are numerous indications that ERU is an immune-mediated disease. We investigated whether neutrophil extracellular traps (NETs) are part of the ERU pathogenesis. Therefore, vitreous body fluids (VBF), sera, and histological sections of the eye from ERU-diseased horses were analyzed for the presence of NET markers and compared with horses with healthy eyes. In addition, NET formation by blood derived neutrophils was investigated in the presence of VBF derived from horses with healthy eyes versus ERU-diseased horses using immunofluorescence microscopy. Interestingly, NET markers like free DNA, histone-complexes, and myeloperoxidase were detected in higher amounts in samples from ERU-diseased horses. Furthermore, in vitro NET formation was higher in neutrophils incubated with VBF from diseased horses compared with those animals with healthy eyes. Finally, we characterized the ability of equine cathelicidins to induce NETs, as potential NET inducing factors in ERU-diseased horses. In summary, our findings lead to the hypothesis that ERU-diseased horses develop more NETs and that these may contribute to the pathogenesis of ERU.
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Publication Date: 2019-11-27 PubMed ID: 31783639PubMed Central: PMC6953072DOI: 10.3390/cells8121528Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study focuses on understanding the role of Neutrophil Extracellular Traps (NETs) in the pathogenesis of Equine Recurrent Uveitis (ERU), an eye disease common in horses. Comparisons were made between ERU-diseased horses and healthy ones, with noticeable increases in NET markers such as free DNA, histone-complexes, and myeloperoxidase in the former.

Introduction

  • The research focuses on Equine Recurrent Uveitis (ERU), a common eye ailment in horses characterized by repeated inflammatory episodes. The disease is often triggered by a variety of disorders, including infections.
  • While ERU appears to have no systemic effects, there’s plenty of evidence suggesting that it’s an immune-mediated disease. The research therefore aimed to investigate whether Neutrophil Extracellular Traps (NETs) contribute to the pathogenesis of ERU.

Methodology

  • The researchers analyzed vitreous body fluids (VBF), sera, and histological sections of the eye from ERU-diseased horses. They specifically looked for the presence of NET markers and compared the findings with those from horses with healthy eyes.
  • They also studied NET formation by neutrophils in the presence of VBF from both ERU-diseased horses and healthy ones using immunofluorescence microscopy.
  • In addition, they explored the ability of equine cathelicidins to induce NETs, as these could potentially act as NET inducing factors in ERU-diseased horses.

Results

  • NET markers such as free DNA, histone-complexes, and myeloperoxidase were found in higher amounts in samples from ERU-diseased horses compared to those with healthy eyes.
  • Also, in vitro NET formation was higher in neutrophils incubated with VBF from ERU-diseased horses compared to those from horses with healthy eyes.

Conclusion

  • The results of the study led researchers to propose that ERU-diseased horses produce more NETs, thereby suggesting that these might play a role in the progression of ERU. However, it’s a hypothesis that needs further investigation and validation before drawing absolute conclusions.

Cite This Article

APA
Fingerhut L, Ohnesorge B, von Borstel M, Schumski A, Strutzberg-Minder K, Mörgelin M, Deeg CA, Haagsman HP, Beineke A, von Köckritz-Blickwede M, de Buhr N. (2019). Neutrophil Extracellular Traps in the Pathogenesis of Equine Recurrent Uveitis (ERU). Cells, 8(12). https://doi.org/10.3390/cells8121528

Publication

Cells
ISSN: 2073-4409
NlmUniqueID: 101600052
Country: Switzerland
Language: English
Volume: 8
Issue: 12

Researcher Affiliations

Fingerhut, Leonie
  • Department of Physiological Chemistry, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany.
  • Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany.
  • Clinic for Horses, University of Veterinary Medicine Hannover, Bünteweg 9, D-30559 Hannover, Germany.
Ohnesorge, Bernhard
  • Clinic for Horses, University of Veterinary Medicine Hannover, Bünteweg 9, D-30559 Hannover, Germany.
von Borstel, Myriam
  • Tierärztliche Gemeinschaftspraxis für Pferde Wedemark, Lange Loh 15, D-30900 Wedemark, Germany.
Schumski, Ariane
  • Department of Physiological Chemistry, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany.
Strutzberg-Minder, Katrin
  • IVD Gesellschaft für Innovative Veterinärdiagnostik mbH (IVD GmbH), Albert-Einstein-Str. 5, D-30926 Seelze, Germany.
Mörgelin, Matthias
  • Colzyx AB, Medicon Village, SE-223 81 Lund, Sweden.
Deeg, Cornelia A
  • Chair of Animal Physiology, Department of Veterinary Sciences, LMU Munich, Lena Christ Str. 48, D-82152 Martinsried, Germany.
Haagsman, Henk P
  • Department of Infectious Diseases & Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands.
Beineke, Andreas
  • Department of Pathology, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany.
von Köckritz-Blickwede, Maren
  • Department of Physiological Chemistry, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany.
  • Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany.
de Buhr, Nicole
  • Department of Physiological Chemistry, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany.
  • Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany.

MeSH Terms

  • Animals
  • Antimicrobial Cationic Peptides / immunology
  • Chronic Disease / veterinary
  • Extracellular Traps / immunology
  • Horses / immunology
  • Uveitis / immunology
  • Uveitis / veterinary
  • Vitreous Body / immunology
  • Vitreous Body / pathology
  • Cathelicidins

Conflict of Interest Statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

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