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Home / Equine Research Bank / PLoS One / Neutrophils are not less sensitive than other blood leukocyt...
PloS one2012; 7(9); e44606; doi: 10.1371/journal.pone.0044606

Neutrophils are not less sensitive than other blood leukocytes to the genomic effects of glucocorticoids.

Abstract: Neutrophils are generally considered less responsive to glucocorticoids compared to other inflammatory cells. The reported increase in human neutrophil survival mediated by these drugs partly supports this assertion. However, it was recently shown that dexamethasone exerts potent anti-inflammatory effects in equine peripheral blood neutrophils. Few comparative studies of glucocorticoid effects in neutrophils and other leukocytes have been reported and a relative insensitivity of neutrophils to these drugs could not be ruled out. Objective: We assessed glucocorticoid-responsiveness in equine and human peripheral blood neutrophils and neutrophil-depleted leukocytes. Methods: Blood neutrophils and neutrophil-depleted leukocytes were isolated from 6 healthy horses and 4 human healthy subjects. Cells were incubated for 5 h with or without LPS (100 ng/mL) alone or combined with hydrocortisone, prednisolone or dexamethasone (10(-8) M and 10(-6) M). IL-1β, TNF-α, IL-8, glutamine synthetase and GR-α mRNA expression was quantified by qPCR. Equine neutrophils were also incubated for 20 h with or without the three glucocorticoids and cell survival was assessed by flow cytometry and light microscopy on cytospin preparations. Results: We found that glucocorticoids down-regulated LPS-induced pro-inflammatory mRNA expression in both cell populations and species. These drugs also significantly increased glutamine synthetase gene expression in both equine cell populations. The magnitude of glucocorticoid response between cell populations was generally similar in both species. We also showed that dexamethasone had a comparable inhibitory effect on pro-inflammatory gene expression in both human and equine neutrophils. As reported in other species, glucocorticoids significantly increase the survival in equine neutrophils. Conclusions: Glucocorticoids exert genomic effects of similar magnitude on neutrophils and on other blood leukocytes. We speculate that the poor response to glucocorticoids observed in some chronic neutrophilic diseases such as severe asthma or COPD is not explained by a relative lack of inhibition of these drugs on pro-inflammatory cytokines expression in neutrophils.
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Publication Date: 2012-09-12 PubMed ID: 22984532PubMed Central: PMC3440353DOI: 10.1371/journal.pone.0044606Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study investigates the effects of glucocorticoids – a type of steroid hormone – on different types of white blood cells, specifically neutrophils. The researchers conclude that neutrophils are not less sensitive to the effects of glucocorticoids compared to other white blood cells, contrary to the common belief.

Objective and Methodology of the Research

  • The study aimed to assess the responsiveness of neutrophils – a type of leukocyte (white blood cell) – to glucocorticoids in equine and human blood.
  • The researchers isolated blood neutrophils and neutrophil-depleted leukocytes from six healthy horses and four humans.
  • The isolated cells were incubated for five hours with or without LPS (a molecule found in bacteria that triggers inflammation) either on its own or in combination with one of three glucocorticoids: hydrocortisone, prednisolone, or dexamethasone. The researchers also carried out a longer, 20-hour incubation with the glucocorticoids for equine neutrophils.
  • The researchers then measured the expression of several genes associated with inflammation and glucocorticoid response using quantitative PCR; a method for amplifying and measuring specific DNA sequences.

Results of the Research

  • The research showed that glucocorticoids down-regulated the expression of genes associated with pro-inflammation in both the neutrophils and the other leukocytes in both humans and horses. This means that the glucocorticoids were able to reduce the inflammatory response in these cells.
  • Moreover, glucocorticoids robustly increased the expression of the glutamine synthetase gene in both types of equine cells. As glutamine synthetase plays a key role in certain pathways related to cellular energy and the immune system, this suggests another way that glucocorticoids may be affecting these cells.
  • The researchers also found that the glucocorticoid dexamethasone was similarly effective at inhibiting the expression of pro-inflammatory genes in both human and equine neutrophils.
  • As observed in other species, glucocorticoids significantly increased the survival rate of equine neutrophils.

Conclusions of the Research

  • The researchers concluded that glucocorticoids have similar effects on neutrophils and other leukocytes, contradicting the current belief that neutrophils are less responsive to these drugs.
  • They suggest that the ineffective response of some chronic neutrophilic diseases like severe asthma or COPD to glucocorticoids is not due to an inherent insensitivity of neutrophils to these drugs.

Cite This Article

APA
Hirsch G, Lavoie-Lamoureux A, Beauchamp G, Lavoie JP. (2012). Neutrophils are not less sensitive than other blood leukocytes to the genomic effects of glucocorticoids. PLoS One, 7(9), e44606. https://doi.org/10.1371/journal.pone.0044606

Publication

PloS one
ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 7
Issue: 9
Pages: e44606
PII: e44606

Researcher Affiliations

Hirsch, Gaelle
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Université de Montréal, Saint-Hyacinthe, Q, Canada.
Lavoie-Lamoureux, Anouk
    Beauchamp, Guy
      Lavoie, Jean-Pierre

        MeSH Terms

        • Adult
        • Animals
        • Anti-Inflammatory Agents / pharmacology
        • Cell Separation
        • Cell Survival
        • Cells, Cultured
        • Dose-Response Relationship, Drug
        • Down-Regulation
        • Female
        • Flow Cytometry / methods
        • Gene Expression Regulation / drug effects
        • Glucocorticoids / metabolism
        • Glucocorticoids / pharmacology
        • Horses
        • Humans
        • Leukocytes / drug effects
        • Lipopolysaccharides / metabolism
        • Neutrophils / drug effects
        • RNA, Messenger / metabolism

        Grant Funding

        • MOP-94848 / Canadian Institutes of Health Research

        Conflict of Interest Statement

        The authors have declared that no competing interests exist.

        References

        This article includes 55 references
        1. Cassatella MA. Neutrophil-derived proteins: selling cytokines by the pound.. Adv Immunol 1999;73:369-509.
          pubmed: 10399011doi: 10.1016/s0065-2776(08)60791-9google scholar: lookup
        2. Filep JG, El Kebir D. Neutrophil apoptosis: a target for enhancing the resolution of inflammation.. J Cell Biochem 2009 Dec 1;108(5):1039-46.
          pubmed: 19760640doi: 10.1002/jcb.22351google scholar: lookup
        3. Schleimer RP. Effects of glucocorticosteroids on inflammatory cells relevant to their therapeutic applications in asthma.. Am Rev Respir Dis 1990 Feb;141(2 Pt 2):S59-69.
          pubmed: 2178515
        4. Schleimer RP. Glucocorticoids suppress inflammation but spare innate immune responses in airway epithelium.. Proc Am Thorac Soc 2004;1(3):222-30.
          pubmed: 16113438doi: 10.1513/pats.200402-018MSgoogle scholar: lookup
        5. Schleimer RP, Freeland HS, Peters SP, Brown KE, Derse CP. An assessment of the effects of glucocorticoids on degranulation, chemotaxis, binding to vascular endothelium and formation of leukotriene B4 by purified human neutrophils.. J Pharmacol Exp Ther 1989 Aug;250(2):598-605.
          pubmed: 2547940
        6. Cox G. Glucocorticoid treatment inhibits apoptosis in human neutrophils. Separation of survival and activation outcomes.. J Immunol 1995 May 1;154(9):4719-25.
          pubmed: 7722324
        7. Meagher LC, Cousin JM, Seckl JR, Haslett C. Opposing effects of glucocorticoids on the rate of apoptosis in neutrophilic and eosinophilic granulocytes.. J Immunol 1996 Jun 1;156(11):4422-8.
          pubmed: 8666816
        8. Barnes PJ, Adcock IM. Glucocorticoid resistance in inflammatory diseases.. Lancet 2009 May 30;373(9678):1905-17.
          pubmed: 19482216doi: 10.1016/S0140-6736(09)60326-3google scholar: lookup
        9. Ordoñez CL, Shaughnessy TE, Matthay MA, Fahy JV. Increased neutrophil numbers and IL-8 levels in airway secretions in acute severe asthma: Clinical and biologic significance.. Am J Respir Crit Care Med 2000 Apr;161(4 Pt 1):1185-90.
          pubmed: 10764310doi: 10.1164/ajrccm.161.4.9812061google scholar: lookup
        10. Wenzel SE, Schwartz LB, Langmack EL, Halliday JL, Trudeau JB, Gibbs RL, Chu HW. Evidence that severe asthma can be divided pathologically into two inflammatory subtypes with distinct physiologic and clinical characteristics.. Am J Respir Crit Care Med 1999 Sep;160(3):1001-8.
          pubmed: 10471631doi: 10.1164/ajrccm.160.3.9812110google scholar: lookup
        11. Wenzel SE, Szefler SJ, Leung DY, Sloan SI, Rex MD, Martin RJ. Bronchoscopic evaluation of severe asthma. Persistent inflammation associated with high dose glucocorticoids.. Am J Respir Crit Care Med 1997 Sep;156(3 Pt 1):737-43.
          pubmed: 9309987doi: 10.1164/ajrccm.156.3.9610046google scholar: lookup
        12. Yang IA, Fong KM, Sim EH, Black PN, Lasserson TJ. Inhaled corticosteroids for stable chronic obstructive pulmonary disease.. Cochrane Database Syst Rev 2007 Apr 18;(2):CD002991.
          pubmed: 17443520doi: 10.1002/14651858.CD002991.pub2google scholar: lookup
        13. Barnes PJ. New molecular targets for the treatment of neutrophilic diseases.. J Allergy Clin Immunol 2007 May;119(5):1055-62; quiz 1063-4.
          pubmed: 17353033doi: 10.1016/j.jaci.2007.01.015google scholar: lookup
        14. al-Mokdad M, Shibata F, Takano K, Nakagawa H. Differential production of chemokines by phagocytosing rat neutrophils and macrophages.. Inflammation 1998 Apr;22(2):145-59.
          pubmed: 9561925doi: 10.1023/a:1022383922039google scholar: lookup
        15. Strandberg K, Blidberg K, Sahlander K, Palmberg L, Larsson K. Effect of formoterol and budesonide on chemokine release, chemokine receptor expression and chemotaxis in human neutrophils.. Pulm Pharmacol Ther 2010 Aug;23(4):316-23.
          pubmed: 20307681doi: 10.1016/j.pupt.2010.03.004google scholar: lookup
        16. Nupponen I, Repo H, Kari A, Pohjavuori M, Andersson S. Early dexamethasone decreases expression of activation markers on neutrophils and monocytes in preterm infants.. Acta Paediatr 2002;91(11):1200-7.
          pubmed: 12463319doi: 10.1080/080352502320777432google scholar: lookup
        17. Wertheim WA, Kunkel SL, Standiford TJ, Burdick MD, Becker FS, Wilke CA, Gilbert AR, Strieter RM. Regulation of neutrophil-derived IL-8: the role of prostaglandin E2, dexamethasone, and IL-4.. J Immunol 1993 Aug 15;151(4):2166-75.
          pubmed: 8345201
        18. Burton JL, Kehrli ME Jr, Kapil S, Horst RL. Regulation of L-selectin and CD18 on bovine neutrophils by glucocorticoids: effects of cortisol and dexamethasone.. J Leukoc Biol 1995 Feb;57(2):317-25.
          pubmed: 7531748doi: 10.1002/jlb.57.2.317google scholar: lookup
        19. Weber PS, Toelboell T, Chang LC, Tirrell JD, Saama PM, Smith GW, Burton JL. Mechanisms of glucocorticoid-induced down-regulation of neutrophil L-selectin in cattle: evidence for effects at the gene-expression level and primarily on blood neutrophils.. J Leukoc Biol 2004 May;75(5):815-27.
          pubmed: 14761937doi: 10.1189/jlb.1003505google scholar: lookup
        20. Lecoq L, Vincent P, Lavoie-Lamoureux A, Lavoie JP. Genomic and non-genomic effects of dexamethasone on equine peripheral blood neutrophils.. Vet Immunol Immunopathol 2009 Mar 15;128(1-3):126-31.
          pubmed: 19036458doi: 10.1016/j.vetimm.2008.10.303google scholar: lookup
        21. Joubert P, Silversides DW, Lavoie JP. Equine neutrophils express mRNA for tumour necrosis factor-alpha, interleukin (IL)-1beta, IL-6, IL-8, macrophage-inflammatory-protein-2 but not for IL-4, IL-5 and interferon-gamma.. Equine Vet J 2001 Nov;33(7):730-3.
          pubmed: 11770998doi: 10.2746/042516401776249246google scholar: lookup
        22. Lavoie-Lamoureux A, Beauchamp G, Quessy S, Martin JG, Lavoie JP. Systemic inflammation and priming of peripheral blood leukocytes persist during clinical remission in horses with heaves.. Vet Immunol Immunopathol 2012 Mar 15;146(1):35-45.
          pubmed: 22342218doi: 10.1016/j.vetimm.2012.01.020google scholar: lookup
        23. Haslett C, Guthrie LA, Kopaniak MM, Johnston RB Jr, Henson PM. Modulation of multiple neutrophil functions by preparative methods or trace concentrations of bacterial lipopolysaccharide.. Am J Pathol 1985 Apr;119(1):101-10.
          pmc: PMC1888083pubmed: 2984939
        24. Lavoie-Lamoureux A, Moran K, Beauchamp G, Mauel S, Steinbach F, Lefebvre-Lavoie J, Martin JG, Lavoie JP. IL-4 activates equine neutrophils and induces a mixed inflammatory cytokine expression profile with enhanced neutrophil chemotactic mediator release ex vivo.. Am J Physiol Lung Cell Mol Physiol 2010 Oct;299(4):L472-82.
          pubmed: 20639353doi: 10.1152/ajplung.00135.2009google scholar: lookup
        25. Fialkow L, Fochesatto Filho L, Bozzetti MC, Milani AR, Rodrigues Filho EM, Ladniuk RM, Pierozan P, de Moura RM, Prolla JC, Vachon E, Downey GP. Neutrophil apoptosis: a marker of disease severity in sepsis and sepsis-induced acute respiratory distress syndrome.. Crit Care 2006;10(6):R155.
          pmc: PMC1794458pubmed: 17092345doi: 10.1186/cc5090google scholar: lookup
        26. Barnes PJ. Glucocorticosteroids: current and future directions.. Br J Pharmacol 2011 May;163(1):29-43.
          pmc: PMC3085866pubmed: 21198556doi: 10.1111/j.1476-5381.2010.01199.xgoogle scholar: lookup
        27. Nicolaides NC, Galata Z, Kino T, Chrousos GP, Charmandari E. The human glucocorticoid receptor: molecular basis of biologic function.. Steroids 2010 Jan;75(1):1-12.
          pmc: PMC2813911pubmed: 19818358doi: 10.1016/j.steroids.2009.09.002google scholar: lookup
        28. Pujols L, Mullol J, Picado C. Alpha and beta glucocorticoid receptors: relevance in airway diseases.. Curr Allergy Asthma Rep 2007 May;7(2):93-9.
          pubmed: 17437678doi: 10.1007/s11882-007-0005-3google scholar: lookup
        29. Dandona P, Mohanty P, Hamouda W, Aljada A, Kumbkarni Y, Garg R. Effect of dexamethasone on reactive oxygen species generation by leukocytes and plasma interleukin-10 concentrations: a pharmacodynamic study.. Clin Pharmacol Ther 1999 Jul;66(1):58-65.
          pubmed: 10430110doi: 10.1016/S0009-9236(99)70054-8google scholar: lookup
        30. Chandrasekhar S, Souba WW, Abcouwer SF. Identification of glucocorticoid-responsive elements that control transcription of rat glutamine synthetase.. Am J Physiol 1999 Feb;276(2):L319-31.
          pubmed: 9950895doi: 10.1152/ajplung.1999.276.2.L319google scholar: lookup
        31. Gaunitz F, Heise K, Schumann R, Gebhardt R. Glucocorticoid induced expression of glutamine synthetase in hepatoma cells.. Biochem Biophys Res Commun 2002 Aug 30;296(4):1026-32.
          pubmed: 12200152doi: 10.1016/s0006-291x(02)02044-2google scholar: lookup
        32. Grossmann C, Scholz T, Rochel M, Bumke-Vogt C, Oelkers W, Pfeiffer AF, Diederich S, Bahr V. Transactivation via the human glucocorticoid and mineralocorticoid receptor by therapeutically used steroids in CV-1 cells: a comparison of their glucocorticoid and mineralocorticoid properties.. Eur J Endocrinol 2004 Sep;151(3):397-406.
          pubmed: 15362971doi: 10.1530/eje.0.1510397google scholar: lookup
        33. Leclere M, Lefebvre-Lavoie J, Beauchamp G, Lavoie JP. Efficacy of oral prednisolone and dexamethasone in horses with recurrent airway obstruction in the presence of continuous antigen exposure.. Equine Vet J 2010 May;42(4):316-21.
          pubmed: 20525049doi: 10.1111/j.2042-3306.2009.00022.xgoogle scholar: lookup
        34. Buttgereit F, Brand MD, Burmester GR. Equivalent doses and relative drug potencies for non-genomic glucocorticoid effects: a novel glucocorticoid hierarchy.. Biochem Pharmacol 1999 Jul 15;58(2):363-8.
          pubmed: 10423179doi: 10.1016/s0006-2952(99)00090-8google scholar: lookup
        35. Grady JA, Davis EG, Kukanich B, Sherck AB. Pharmacokinetics and pharmacodynamics of dexamethasone after oral administration in apparently healthy horses.. Am J Vet Res 2010 Jul;71(7):831-9.
          pubmed: 20594087doi: 10.2460/ajvr.71.7.831google scholar: lookup
        36. Czock D, Keller F, Rasche FM, Häussler U. Pharmacokinetics and pharmacodynamics of systemically administered glucocorticoids.. Clin Pharmacokinet 2005;44(1):61-98.
          pubmed: 15634032doi: 10.2165/00003088-200544010-00003google scholar: lookup
        37. Xu J, Winkler J, Derendorf H. A pharmacokinetic/pharmacodynamic approach to predict total prednisolone concentrations in human plasma.. J Pharmacokinet Pharmacodyn 2007 Jun;34(3):355-72.
          pubmed: 17318442doi: 10.1007/s10928-007-9050-8google scholar: lookup
        38. Wałajtys-Rode E, Dabrowski A, Grubek-Jaworska H, Machnicka B, Droszcz W. Binding of dexamethasone and its effect on histamine release from rat mast cells.. Int J Immunopharmacol 1988;10(8):925-30.
          pubmed: 2463972doi: 10.1016/0192-0561(88)90038-0google scholar: lookup
        39. Burrows GE. Dose-response of ponies to parenteral Escherichia coli endotoxin.. Can J Comp Med 1981 Apr;45(2):207-10.
          pmc: PMC1320155pubmed: 7020894
        40. Berczi I, Bertók L, Bereznai T. Comparative studies on the toxicity of Escherichia coli lipopolysaccharide endotoxin in various animal species.. Can J Microbiol 1966 Oct;12(5):1070-1.
          pubmed: 5339644doi: 10.1139/m66-143google scholar: lookup
        41. Pålsson-McDermott EM, O'Neill LA. Signal transduction by the lipopolysaccharide receptor, Toll-like receptor-4.. Immunology 2004 Oct;113(2):153-62.
          pmc: PMC1782563pubmed: 15379975doi: 10.1111/j.1365-2567.2004.01976.xgoogle scholar: lookup
        42. Sabroe I, Jones EC, Usher LR, Whyte MK, Dower SK. Toll-like receptor (TLR)2 and TLR4 in human peripheral blood granulocytes: a critical role for monocytes in leukocyte lipopolysaccharide responses.. J Immunol 2002 May 1;168(9):4701-10.
          pubmed: 11971020doi: 10.4049/jimmunol.168.9.4701google scholar: lookup
        43. Wang XQ, Zhou X, Zhou Y, Rong L, Gao L, Xu W. Low-dose dexamethasone alleviates lipopolysaccharide-induced acute lung injury in rats and upregulates pulmonary glucocorticoid receptors.. Respirology 2008 Nov;13(6):772-80.
          pubmed: 18657064doi: 10.1111/j.1440-1843.2008.01344.xgoogle scholar: lookup
        44. Cassatella MA. The production of cytokines by polymorphonuclear neutrophils.. Immunol Today 1995 Jan;16(1):21-6.
          pubmed: 7880385doi: 10.1016/0167-5699(95)80066-2google scholar: lookup
        45. Saffar AS, Ashdown H, Gounni AS. The molecular mechanisms of glucocorticoids-mediated neutrophil survival.. Curr Drug Targets 2011 Apr;12(4):556-62.
          pmc: PMC3267167pubmed: 21504070doi: 10.2174/138945011794751555google scholar: lookup
        46. Saffar AS, Dragon S, Ezzati P, Shan L, Gounni AS. Phosphatidylinositol 3-kinase and p38 mitogen-activated protein kinase regulate induction of Mcl-1 and survival in glucocorticoid-treated human neutrophils.. J Allergy Clin Immunol 2008 Feb;121(2):492-498.e10.
          pubmed: 18036649doi: 10.1016/j.jaci.2007.10.003google scholar: lookup
        47. Colotta F, Re F, Polentarutti N, Sozzani S, Mantovani A. Modulation of granulocyte survival and programmed cell death by cytokines and bacterial products.. Blood 1992 Oct 15;80(8):2012-20.
          pubmed: 1382715
        48. Langereis JD, Oudijk EJ, Schweizer RC, Lammers JW, Koenderman L, Ulfman LH. Steroids induce a disequilibrium of secreted interleukin-1 receptor antagonist and interleukin-1β synthesis by human neutrophils.. Eur Respir J 2011 Feb;37(2):406-15.
          pubmed: 20650986doi: 10.1183/09031936.00170409google scholar: lookup
        49. Cohen PR. Sweet's syndrome--a comprehensive review of an acute febrile neutrophilic dermatosis.. Orphanet J Rare Dis 2007 Jul 26;2:34.
          pmc: PMC1963326pubmed: 17655751doi: 10.1186/1750-1172-2-34google scholar: lookup
        50. Leclere M, Lavoie-Lamoureux A, Lavoie JP. Heaves, an asthma-like disease of horses.. Respirology 2011 Oct;16(7):1027-46.
          pubmed: 21824219doi: 10.1111/j.1440-1843.2011.02033.xgoogle scholar: lookup
        51. Lavoie JP, Pasloske K, Joubert P, Cordeau ME, Mancini J, Girard Y, Friesen RW, Frenette R, Blouin M, Young RN, Hickey G. Lack of clinical efficacy of a phosphodiesterase-4 inhibitor for treatment of heaves in horses.. J Vet Intern Med 2006 Jan-Feb;20(1):175-81.
          pubmed: 16496938doi: 10.1892/0891-6640(2006)20[175:loceoa]2.0.co;2google scholar: lookup
        52. Lavoie JP, Léguillette R, Pasloske K, Charette L, Sawyer N, Guay D, Murphy T, Hickey GJ. Comparison of effects of dexamethasone and the leukotriene D4 receptor antagonist L-708,738 on lung function and airway cytologic findings in horses with recurrent airway obstruction.. Am J Vet Res 2002 Apr;63(4):579-85.
          pubmed: 11939323doi: 10.2460/ajvr.2002.63.579google scholar: lookup
        53. Barnes PJ. Mechanisms and resistance in glucocorticoid control of inflammation.. J Steroid Biochem Mol Biol 2010 May 31;120(2-3):76-85.
          pubmed: 20188830doi: 10.1016/j.jsbmb.2010.02.018google scholar: lookup
        54. Strickland I, Kisich K, Hauk PJ, Vottero A, Chrousos GP, Klemm DJ, Leung DY. High constitutive glucocorticoid receptor beta in human neutrophils enables them to reduce their spontaneous rate of cell death in response to corticosteroids.. J Exp Med 2001 Mar 5;193(5):585-93.
          pmc: PMC2193396pubmed: 11238589doi: 10.1084/jem.193.5.585google scholar: lookup
        55. Vazquez-Tello A, Semlali A, Chakir J, Martin JG, Leung DY, Eidelman DH, Hamid Q. Induction of glucocorticoid receptor-beta expression in epithelial cells of asthmatic airways by T-helper type 17 cytokines.. Clin Exp Allergy 2010 Sep;40(9):1312-22.
          pubmed: 20545708doi: 10.1111/j.1365-2222.2010.03544.xgoogle scholar: lookup

        Citations

        This article has been cited 22 times.
        1. Eddins DJ, Yang J, Kosters A, Giacalone VD, Pechuan-Jorge X, Chandler JD, Eum J, Babcock BR, Dobosh BS, Hernández MR, Abdulkhader F, Collins GL, Orlova DY, Ramonell RP, Sanz I, Moussion C, Eun-Hyung Lee F, Tirouvanziam RM, Ghosn EEB. Transcriptional reprogramming of infiltrating neutrophils drives lung pathology in severe COVID-19 despite low viral load.. Blood Adv 2023 Mar 14;7(5):778-799.
          doi: 10.1182/bloodadvances.2022008834pubmed: 36399523google scholar: lookup
        2. Wang X, Tang M, Zhang Y, Li Y, Mao J, Deng Q, Li S, Jia Z, Du L. Dexamethasone enhances glucose uptake by SGLT1 and GLUT1 and boosts ATP generation through the PPP-TCA cycle in bovine neutrophils.. J Vet Sci 2022 Sep;23(5):e76.
          doi: 10.4142/jvs.22112pubmed: 36174980google scholar: lookup
        3. Jia WY, Zhang JJ. Effects of glucocorticoids on leukocytes: Genomic and non-genomic mechanisms.. World J Clin Cases 2022 Jul 26;10(21):7187-7194.
          doi: 10.12998/wjcc.v10.i21.7187pubmed: 36158016google scholar: lookup
        4. Murru A, Allard MÈ, Paré G, Vaillancourt M, Boyer L, Cayer MP, Vitry J, Landry P, Labrecque MM, Robitaille N, Branch DR, Girard M, Fernandes MJ. Comparison of Neutrophil Function in Granulocyte Concentrates From Prednisone- and G-CSF-Treated Donors: Effect of Stimulant, Leukapheresis and Storage.. Front Med (Lausanne) 2022;9:839475.
          doi: 10.3389/fmed.2022.839475pubmed: 35317326google scholar: lookup
        5. Caillon A, Trimaille A, Favre J, Jesel L, Morel O, Kauffenstein G. Role of neutrophils, platelets, and extracellular vesicles and their interactions in COVID-19-associated thrombopathy.. J Thromb Haemost 2022 Jan;20(1):17-31.
          doi: 10.1111/jth.15566pubmed: 34672094google scholar: lookup
        6. Mainguy-Seers S, Lavoie JP. Glucocorticoid treatment in horses with asthma: A narrative review.. J Vet Intern Med 2021 Jul;35(4):2045-2057.
          doi: 10.1111/jvim.16189pubmed: 34085342google scholar: lookup
        7. Zaza G, Leventhal J, Signorini L, Gambaro G, Cravedi P. Effects of Antirejection Drugs on Innate Immune Cells After Kidney Transplantation.. Front Immunol 2019;10:2978.
          doi: 10.3389/fimmu.2019.02978pubmed: 31921213google scholar: lookup
        8. Flynn JK, Dankers W, Morand EF. Could GILZ Be the Answer to Glucocorticoid Toxicity in Lupus?. Front Immunol 2019;10:1684.
          doi: 10.3389/fimmu.2019.01684pubmed: 31379872google scholar: lookup
        9. Cari L, De Rosa F, Nocentini G, Riccardi C. Context-Dependent Effect of Glucocorticoids on the Proliferation, Differentiation, and Apoptosis of Regulatory T Cells: A Review of the Empirical Evidence and Clinical Applications.. Int J Mol Sci 2019 Mar 6;20(5).
          doi: 10.3390/ijms20051142pubmed: 30845709google scholar: lookup
        10. Ronchetti S, Ricci E, Migliorati G, Gentili M, Riccardi C. How Glucocorticoids Affect the Neutrophil Life.. Int J Mol Sci 2018 Dec 17;19(12).
          doi: 10.3390/ijms19124090pubmed: 30563002google scholar: lookup
        11. Lavoie JP, Leclere M, Rodrigues N, Lemos KR, Bourzac C, Lefebvre-Lavoie J, Beauchamp G, Albrecht B. Efficacy of inhaled budesonide for the treatment of severe equine asthma.. Equine Vet J 2019 May;51(3):401-407.
          doi: 10.1111/evj.13018pubmed: 30203854google scholar: lookup
        12. Carvalho VF, Barreto EO, Arantes ACS, Serra MF, Ferreira TPT, Jannini-Sá YAP, Hogaboam CM, Martins MA, Silva PMR. Diabetes Downregulates Allergen-Induced Airway Inflammation in Mice.. Mediators Inflamm 2018;2018:6150843.
          doi: 10.1155/2018/6150843pubmed: 29849493google scholar: lookup
        13. Jonasson L, Grauen Larsen H, Lundberg AK, Gullstrand B, Bengtsson AA, Schiopu A. Stress-induced release of the S100A8/A9 alarmin is elevated in coronary artery disease patients with impaired cortisol response.. Sci Rep 2017 Dec 13;7(1):17545.
          doi: 10.1038/s41598-017-17586-6pubmed: 29235502google scholar: lookup
        14. Vargas A, Boivin R, Cano P, Murcia Y, Bazin I, Lavoie JP. Neutrophil extracellular traps are downregulated by glucocorticosteroids in lungs in an equine model of asthma.. Respir Res 2017 Dec 12;18(1):207.
          doi: 10.1186/s12931-017-0689-4pubmed: 29233147google scholar: lookup
        15. Herteman N, Vargas A, Lavoie JP. Characterization of Circulating Low-Density Neutrophils Intrinsic Properties in Healthy and Asthmatic Horses.. Sci Rep 2017 Aug 10;7(1):7743.
          doi: 10.1038/s41598-017-08089-5pubmed: 28798364google scholar: lookup
        16. Chen L, Eapen MS, Zosky GR. Vitamin D both facilitates and attenuates the cellular response to lipopolysaccharide.. Sci Rep 2017 Mar 27;7:45172.
          doi: 10.1038/srep45172pubmed: 28345644google scholar: lookup
        17. Coutinho AE, Kipari TM, Zhang Z, Esteves CL, Lucas CD, Gilmour JS, Webster SP, Walker BR, Hughes J, Savill JS, Seckl JR, Rossi AG, Chapman KE. 11β-Hydroxysteroid Dehydrogenase Type 1 Is Expressed in Neutrophils and Restrains an Inflammatory Response in Male Mice.. Endocrinology 2016 Jul;157(7):2928-36.
          doi: 10.1210/en.2016-1118pubmed: 27145012google scholar: lookup
        18. Murcia RY, Vargas A, Lavoie JP. The Interleukin-17 Induced Activation and Increased Survival of Equine Neutrophils Is Insensitive to Glucocorticoids.. PLoS One 2016;11(5):e0154755.
          doi: 10.1371/journal.pone.0154755pubmed: 27138006google scholar: lookup
        19. Hirz T, Dumontet C. Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents.. J Vis Exp 2016 Mar 25;(109):e53846.
          doi: 10.3791/53846pubmed: 27078837google scholar: lookup
        20. Bao A, Li F, Zhang M, Chen Y, Zhang P, Zhou X. Impact of ozone exposure on the response to glucocorticoid in a mouse model of asthma: involvements of p38 MAPK and MKP-1.. Respir Res 2014 Oct 8;15(1):126.
          doi: 10.1186/s12931-014-0126-xpubmed: 25287866google scholar: lookup
        21. Brazil TJ, Dixon PM, Haslett C, Murray J, McGorum BC. Constitutive apoptosis in equine peripheral blood neutrophils in vitro.. Vet J 2014 Dec;202(3):536-42.
          doi: 10.1016/j.tvjl.2014.08.029pubmed: 25239298google scholar: lookup
        22. Tintinger GR, Anderson R, Feldman C. Pharmacological approaches to regulate neutrophil activity.. Semin Immunopathol 2013 Jul;35(4):395-409.
          doi: 10.1007/s00281-013-0366-8pubmed: 23494251google scholar: lookup
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