New flow cytometry approaches in equine andrology.
Abstract: Flow cytometry is currently recognized as a robust tool for the evaluation of sperm quality and function. However, within equine reproduction, this technique has not reached the sophistication of other areas of biology and medicine. In recent years, more sophisticated flow cytometers have been introduced in andrology laboratories, and the number of tests that can be potentially used in the evaluation of sperm physiology has increased accordingly. In this review, recent advances in the evaluation of stallion spermatozoa will be discussed. These new techniques in flow cytometry are able to simultaneously measure damage to different sperm regions and/or changes in functionality.
Copyright © 2016 Elsevier Inc. All rights reserved.
Publication Date: 2016-04-21 PubMed ID: 27160445DOI: 10.1016/j.theriogenology.2016.04.050Google Scholar: Lookup
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- Journal Article
- Review
Summary
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The research explores the advancements in flow cytometry for assessing sperm quality and function in equine reproduction, particularly in stallions. Despite its prevalent use in other biological and medical fields, the application of flow cytometry is not yet as advanced in equine reproduction.
Introduction to Flow Cytometry in Equine Andrology
- Equine andrology is the branch of science dedicated to male horse reproduction. The research in question is aimed at elevating the use of flow cytometry in this field.
- Flow cytometry is a technique used to measure the physical and chemical characteristics of particles in a fluid as it passes through a laser. It is often employed to sort, count, and examine cells in a sample.
- Despite being established as a robust method for evaluating sperm quality in various species, its application in equine reproduction is relatively less sophisticated.
Recent Developments in Flow Cytometry
- The paper discusses how more advanced flow cytometers have recently been introduced in andrology labs, leading to an increase in potential tests for sperm physiology evaluation.
- New techniques in flow cytometry can simultaneously measure damage to different regions of sperm and changes in functionality. This upgrade allows for a more thorough and accurate assessment of sperm viability and health.
Impact on Stallion Sperm Evaluation
- The research underlines that these novel developments within flow cytometry have specific relevance to stallion sperm evaluation. Due to the specifics of equine reproduction, with certain peculiarities and challenges, sophisticated techniques for sperm quality determination are essential.
- By enabling a more nuanced and comprehensive analysis of sperm physiology, these developments promise to help improve stallion fertility evaluation and breeding management, thus positively impacting equine reproduction.
Cite This Article
APA
Peña FJ, Ortega Ferrusola C, Martín Muñoz P.
(2016).
New flow cytometry approaches in equine andrology.
Theriogenology, 86(1), 366-372.
https://doi.org/10.1016/j.theriogenology.2016.04.050 Publication
Researcher Affiliations
- University of Extremadura, Laboratory of Equine Reproduction and Spermatology, University of Extremadura, Cáceres, Spain. Electronic address: fjuanpvega@unex.es.
- Reproduction and Obstetrics, Department of Animal Medicine and Surgery, University of León, León, Spain.
- University of Extremadura, Laboratory of Equine Reproduction and Spermatology, University of Extremadura, Cáceres, Spain.
MeSH Terms
- Animals
- Flow Cytometry / methods
- Flow Cytometry / veterinary
- Horses / physiology
- Male
- Semen Analysis / veterinary
Citations
This article has been cited 7 times.- Soria-Meneses PJ, Jurado-Campos A, Gómez-Rubio V, Sánchez-Ajofrín I, Soler AJ, Garde JJ, Fernández-Santos MDR. Determination of Ram (Ovis aries) Sperm DNA Damage Due to Oxidative Stress: 8-OHdG Immunodetection Assay vs. SCSA(®). Animals (Basel) 2022 Nov 25;12(23).
- Neila-Montero M, Riesco MF, Alvarez M, Montes-Garrido R, Boixo JC, de Paz P, Anel-Lopez L, Anel L. Centrifugal force assessment in ram sperm: identifying species-specific impact. Acta Vet Scand 2021 Nov 4;63(1):42.
- Jäkel H, Scheinpflug K, Mühldorfer K, Gianluppi R, Lucca MS, Mellagi APG, Bortolozzo FP, Waberski D. In vitro performance and in vivo fertility of antibiotic-free preserved boar semen stored at 5 °C. J Anim Sci Biotechnol 2021 Jan 11;12(1):9.
- F Riesco M, Anel-Lopez L, Neila-Montero M, Palacin-Martinez C, Montes-Garrido R, Alvarez M, de Paz P, Anel L. ProAKAP4 as Novel Molecular Marker of Sperm Quality in Ram: An Integrative Study in Fresh, Cooled and Cryopreserved Sperm. Biomolecules 2020 Jul 14;10(7).
- Ortiz-Rodriguez JM, Anel-Lopez L, Martín-Muñoz P, Álvarez M, Gaitskell-Phillips G, Anel L, Rodríguez-Medina P, Peña FJ, Ortega Ferrusola C. Pulse Doppler ultrasound as a tool for the diagnosis of chronic testicular dysfunction in stallions. PLoS One 2017;12(5):e0175878.
- Loddo G, Gelain ME, Gabai G, D'Andrea A, Montanari E, Milani C, Giaretta E. Mitochondrial function and reactive oxygen species dynamics in Italian Mediterranean buffalo semen following cryopreservation and post-thaw incubation. Front Vet Sci 2025;12:1733446.
- Strassner FM, Demattio L, Siuda M, Malama E, Muffels G, Bollwein H. Relationships Between Metabolism of Cryopreserved Equine Sperm Determined by the Seahorse Analyzer and Sperm Characteristics Measured by Flow Cytometry and Computer-Assisted Analysis of Motility. Vet Sci 2025 Nov 21;12(12).
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