New methods for isolation of keratolytic bacteria inducing intractable hoof wall cavity (Gidoh) in a horse; double screening procedures of the horn powder agar-translucency test and horn zymography.
Abstract: To establish a new system to isolate keratolytic bacteria from the hoof wall cavity () of a racehorse, we invented the horn powder agar-translucency (HoPAT) test and horn zymography (HZ). Using routine bacteriological techniques and these methods, we isolated five strains of keratolytic soil bacteria, which were then identified by means of 16S ribosomal RNA (rRNA) gene sequencing analysis. The findings from the study on the horse suggested that Brevibacterium luteolum played the main role in the local fragility of the hoof, eventually forming a in coordination with four other strains of keratolytic bacteria. The double screening procedures of the HoPAT test and HZ were useful and easy techniques for isolating the keratolytic bacteria from the horn lesions.
Publication Date: 2017-03-28 PubMed ID: 28400703PubMed Central: PMC5383627DOI: 10.1294/jes.28.19Google Scholar: Lookup
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Summary
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The article describes the development of a new system for isolating bacteria that cause hoof wall cavities in racehorses. The researchers identified a specific bacterium, Brevibacterium luteolum, as a key agent causing these cavities.
Development of New Isolation Methods
- Researchers aimed to establish a new system for isolating keratolytic bacteria from horse hoof wall cavities, which cause a condition referred to as . This process involved the creation of two methods: the Horn Powder Agar-Translucency (HoPAT) test and Horn Zymography (HZ).
- These methods were developed in response to the need for effective and easy ways to isolate these bacteria from the afflicted areas, given the challenge they pose to racehorse health and performance.
Identification of Keratolytic Bacteria
- Using a combination of these new techniques and routine bacteriological processes, the study successfully isolated five strains of keratolytic bacteria from soil.
- The identification of these strains was accomplished through 16S ribosomal RNA (rRNA) gene sequencing analysis. This method is commonly used in microbiology to study bacterial genetics and to categorize unidentified strains of bacteria.
Role of Brevibacterium Luteolum
- The study’s findings indicated that one bacteria strain, Brevibacterium luteolum, was mainly responsible for causing the local fragility in the horse hoof, leading to the formation of . It appears to work synergistically with the other four isolated strains to induce this condition.
Utility of HoPAT and HZ
- The combination of the Horn Powder Agar-Translucency (HoPAT) test and Horn Zymography (HZ) proved to be effective in isolating these bacteria.
- These double screening procedures were found to be straightforward and useful techniques for extracting and identifying the keratolytic bacteria from the horn lesions, and thus, could be instrumental in further studies and treatment strategies for this hoof condition.
Cite This Article
APA
Kuwano A, Niwa H, Arai K.
(2017).
New methods for isolation of keratolytic bacteria inducing intractable hoof wall cavity (Gidoh) in a horse; double screening procedures of the horn powder agar-translucency test and horn zymography.
J Equine Sci, 28(1), 19-25.
https://doi.org/10.1294/jes.28.19 Publication
Researcher Affiliations
- Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan; Current affiliation: Japan Farriery Association, Tokyo 105-0004, Japan.
- Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
- Department of Tissue Physiology, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
References
This article includes 28 references
- Apprich V, Spergser J, Rosengarten R, Hinterhofer C, Stanek C. Scanning electron microscopy and fungal culture of hoof horn from horses suffering from onychomycosis.. Vet Dermatol 2010 Aug;21(4):335-40.
- Booth L, White D. Pathological conditions of the external hoof capsule. 2007 pp. 224–252.
- Bora N, Vancanneyt M, Gelsomino R, Swings J, Brennan N, Cogan TM, Larpin S, Desmasures N, Lechner FE, Kroppenstedt RM, Ward AC, Goodfellow M. Agrococcus casei sp. nov., isolated from the surfaces of smear-ripened cheeses.. Int J Syst Evol Microbiol 2007 Jan;57(Pt 1):92-97.
- Brownrigg R, Kempson SE, Robb R, Wildenstein M. Infections of the hoof. 2006 pp. 427–452.
- Budras KD, Schiel C, Mulling C. Horn tubules of the white line: an insufficient barrier against ascending bacterial invasion. Equine Vet. Educ. 1998 10: 81–85.
- Butler D. Hoof moisture. 1985 pp. 138–139.
- Gregory NG. Swelling of cattle heel horn by urine.. Aust Vet J 2004 Mar;82(3):161-3.
- Gregory N, Craggs L, Hobson N, Krogh C. Softening of cattle hoof soles and swelling of heel horn by environmental agents.. Food Chem Toxicol 2006 Aug;44(8):1223-7.
- Higuchi H, Kurumado H, Mori M, Degawa A, Fujisawa H, Kuwano A, Nagahata H. Effects of ammonia and hydrogen sulfide on physical and biochemical properties of the claw horn of Holstein cows.. Can J Vet Res 2009 Jan;73(1):15-20.
- Hiraishi A. Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification.. Lett Appl Microbiol 1992 Nov;15(5):210-3.
- Hiraishi A, Shin YK, Ueda Y, Sugiyama J. Automated sequencing of PCR-amplified 16S rDNA on ‘Hydrolink’ gels. J. Microbiol. Methods 1994 19: 145–154.
- Keller M, Krehon S, Stanek C, Rosengarten R. Keratinopathogenic mould fungi and dermatophytes in healthy and diseased hooves of horses.. Vet Rec 2000 Nov 25;147(22):619-22.
- Kempson SA, Robb R. Use of a topical disinfectant as part of a hoof care programme for horses with diseases of the hoof capsule.. Vet Rec 2004 May 22;154(21):647-52.
- Killham K, Prosser JI. The bacteria and archaea. 2015 pp. 41–76.
- Kuwano A, Oikawa M, Takatori K. Pathomorphological findings in a case of onychomycosis of a racehorse.. J Vet Med Sci 1996 Nov;58(11):1117-20.
- Kuwano A, Yamauchi Y, Sasagawa T, Sasaki N, Hamano H. Epidemiological survey of the hoof wall cavity ('Gidoh' in Japanese) in racehorses.. Vet Rec 2012 Dec 15;171(24):623.
- Kuwano A, Yoshihara T, Takatori K, Kosuge J. Onychomycosis in white line disease in horses: pathology, mycology and clinical features.. Equine Vet J Suppl 1998 Sep;(26):27-35.
- Moyer W. Hoof wall defects: chronic hoof wall separations and hoof wall cracks.. Vet Clin North Am Equine Pract 2003 Aug;19(2):463-77.
- O’Grady SE. White line disease−an update. Equine Vet. Educ. 2002 14: 51–55.
- Oke RA. Unilateral white line disease and laminitis in a quarter horse mare.. Can Vet J 2003 Feb;44(2):145-6.
- Onishi JC, Park JW, Häggblom MM, Fennell MJ, Fugaro MN. Chronic laminitis is associated with potential bacterial pathogens in the laminae.. Vet Microbiol 2012 Aug 17;158(3-4):329-36.
- Renaud FN, Aubel D, Riegel P, Meugnier H, Bollet C. Corynebacterium freneyi sp. nov., alpha-glucosidase-positive strains related to Corynebacterium xerosis.. Int J Syst Evol Microbiol 2001 Sep;51(Pt 5):1723-1728.
- Schubert K, Ludwig W, Springer N, Kroppenstedt RM, Accolas JP, Fiedler F. Two coryneform bacteria isolated from the surface of French Gruyère and Beaufort cheeses are new species of the genus Brachybacterium: Brachybacterium alimentarium sp. nov. and Brachybacterium tyrofermentans sp. nov.. Int J Syst Bacteriol 1996 Jan;46(1):81-7.
- Tanaka D, Takashima M, Mizuta A, Tanaka S, Sakatoku A, Nishikawa A, Osawa T, Noguchi M, Aizawa S, Nakamura S. Acinetobacter sp. Ud-4 efficiently degrades both edible and mineral oils: isolation and characterization.. Curr Microbiol 2010 Mar;60(3):203-9.
- Wattle O. Cytokeratins of the equine hoof wall, chestnut and skin: bio- and immunohisto-chemistry.. Equine Vet J Suppl 1998 Sep;(26):66-80.
- Wauters G, Avesani V, Laffineur K, Charlier J, Janssens M, Van Bosterhaut B, Delmée M. Brevibacterium lutescens sp. nov., from human and environmental samples.. Int J Syst Evol Microbiol 2003 Sep;53(Pt 5):1321-1325.
- Wauters G, Haase G, Avesani V, Charlier J, Janssens M, Van Broeck J, Delmée M. Identification of a novel Brevibacterium species isolated from humans and description of Brevibacterium sanguinis sp. nov.. J Clin Microbiol 2004 Jun;42(6):2829-32.
- Yassin AF, Siering C. Corynebacterium sputi sp. nov., isolated from the sputum of a patient with pneumonia.. Int J Syst Evol Microbiol 2008 Dec;58(Pt 12):2876-9.
Citations
This article has been cited 1 times.- Lin SR, Mokgautsi N, Liu YN. Ras and Wnt Interaction Contribute in Prostate Cancer Bone Metastasis. Molecules 2020 May 20;25(10).
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