Nicoletella semolina in the airways of healthy horses and horses with severe asthma.
Abstract: Nicoletella semolina was identified in the airways of horses and its low prevalence could be because of its difficult differentiation from other Pasteurellaceae. Objective: To develop a molecular method for the identification of N. semolina and to evaluate its prevalence in the mouth and the airways of healthy and severe asthmatic horses. Methods: Six healthy and 6 severely asthmatic horses in phase I, 10 severely asthmatic horses in phase II, and 10 healthy horses in phase III. Methods: Cohort (phases I and II) and cross-sectional (phase III) studies. Quantitative polymerase chain reaction primers targeting the sodA gene were optimized. N. semolina was quantified in oral and nasal washes and in bronchoalveolar lavage fluid (BALF; phase I, sampled twice), in nasal washes and BALF (phase II, sampled twice), and in nasal washes (phase III). Results: N. semolina was found in the nose of 5, 10, and 9 horses in phases I, II, and III, respectively (first sampling for phases I and II). Six BALF from 5 different horses were positive for N. semolina in phase II. In phase I, there was no significant difference in the nasal loads of healthy horses (median (range): 2.04 × 10 copies/mL (0-2.44 × 10 )) and asthmatic horses in exacerbation (3.75 × 10 (0-4.84 × 10 ); Wilcoxon's rank sum test, P = .57). Conclusions: N. semolina is commonly found in the airways of horses. The potential pathogenicity of N. semolina remains to be elucidated, but the molecular technique we developed will facilitate future studies.
© 2021 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.
Publication Date: 2021-05-04 PubMed ID: 33942932PubMed Central: PMC8163135DOI: 10.1111/jvim.16140Google Scholar: Lookup
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Summary
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This research study provides a scientific method for identifying the presence of Nicoletella semolina bacteria in horse airways and evaluates the prevalence among healthy and asthmatic horses. The findings show that N. semolina is frequently found in horses, though its potential effects on health are yet to be determined.
Objective of the research
- This research aimed to design a precise molecular method to identify Nicoletella semolina, a bacterium that has been detected in the horse’s airways, and its commonness among healthy and severely asthmatic horses. The N. semolina bacteria are difficult to differentiate from other Pasteurellaceae bacteria due to their low prevalence.
Methodology
- The researchers implemented a cohort and cross-sectional study. The study participants included six healthy and six severely asthmatic horses in phase I, ten asthmatic horses in phase II, and ten healthy horses in phase III.
- To differentiate N. semolina from other bacteria, they optimized quantitative polymerase chain reaction primers targeting the sodA gene. The amount of N. semolina was measured in the oral and nasal washes, and bronchoalveolar lavage fluid (BALF), a tool for diagnosing lung diseases, especially in phases I and II. In phase III, only nasal washes were used.
Findings
- The results exhibited the presence of N. semolina in both the oral and nasal washes of the horses from all the phases of the study. Six bronchoalveolar lavage fluid from five different horses were positive for N. semolina in phase II.
- The study found no significant difference in bacterial load between healthy horses and asthmatic horses during an exacerbation. This suggests that the presence of N. semolina didn’t correlate with the health condition of the horses.
Conclusion
- The research successfully developed a method to identify N. semolina in horses’ airways. It confirmed that N. semolina is a common bacterium in horses; however, its pathogenicity remains unknown. The developed technique is expected to help future research regarding N. semolina.
Cite This Article
APA
Payette F, Charlebois A, Fairbrother JH, Beauchamp G, Leclere M.
(2021).
Nicoletella semolina in the airways of healthy horses and horses with severe asthma.
J Vet Intern Med, 35(3), 1612-1619.
https://doi.org/10.1111/jvim.16140 Publication
Researcher Affiliations
- Clinical Sciences Department, Faculté de Médecine Vétérinaire, Université de Montréal, Montreal, Q, Canada.
- Clinical Sciences Department, Faculté de Médecine Vétérinaire, Université de Montréal, Montreal, Q, Canada.
- Bacteriology Diagnostic Laboratory, Complexe de Diagnostic et d'Épidémiosurveillance Vétérinaires du Québec, Ministère de l'Agriculture, des Pêcheries et de l'Alimentation du Québec and Faculté de Médecine Vétérinaire, Université de Montréal, Montreal, Q, Canada.
- Veterinary Biomedicine Department, Faculté de Médecine Vétérinaire, Université de Montréal, Montreal, Q, Canada.
- Clinical Sciences Department, Faculté de Médecine Vétérinaire, Université de Montréal, Montreal, Q, Canada.
MeSH Terms
- Animals
- Asthma / veterinary
- Bronchoalveolar Lavage Fluid
- Cross-Sectional Studies
- Horse Diseases
- Horses
- Pasteurellaceae
Grant Funding
- 06090 / Natural Sciences and Engineering Research Council of Canada
Conflict of Interest Statement
Authors declare no conflict of interest.
References
This article includes 48 references
Citations
This article has been cited 4 times.- Liu G, Wang K, Yang Z, Tang X, Chang Y, Dai K, Tang X, Hu B, Zhang Y, Cao S, Huang X, Yan Q, Wu R, Zhao Q, Du S, Wen X, Wen Y. Identification of a Novel Linear B-Cell Epitope of HbpA Protein from Glaesserella parasuis Using Monoclonal Antibody.. Int J Mol Sci 2023 May 12;24(10).
- Bond S, McMullen C, Timsit E, Léguillette R. Topography of the respiratory, oral, and guttural pouch bacterial and fungal microbiotas in horses.. J Vet Intern Med 2023 Jan;37(1):349-360.
- Chaiyasut C, Sivamaruthi BS, Bharathi M, Tansrisook C, Peerajan S, Chaiyasut K, Khongtan S, Tanongpitchayes K, Thongma N, Chawnan N, Thongkorn K. Influence of Gallic Acid-Containing Mouth Spray on Dental Health and Oral Microbiota of Healthy Cats-A Pilot Study.. Vet Sci 2022 Jun 22;9(7).
- Simões J, Batista M, Tilley P. The Immune Mechanisms of Severe Equine Asthma-Current Understanding and What Is Missing.. Animals (Basel) 2022 Mar 16;12(6).
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