Nitric oxide production by equine articular cells in vitro.
Abstract: Recent research in several species has suggested nitric oxide (NO) as a mediator of articular cartilage damage and an inhibitor of cartilage matrix neosynthesis. This study investigated NO production by cultured equine articular chondrocytes in response to 2 arthritogenic molecules, namely lipopolysaccharide (LPS) and interleukin-1 beta (IL-1 beta), and compared NO production by cultured equine synoviocytes stimulated with LPS. Synoviocytes exhibited a low basal level of NO synthesis (measured as nitrite, a NO metabolite) that was neither significantly increased nor decreased by exposure to LPS. Basal NO synthesis by synoviocytes was not significantly reduced by competitive inhibitors of nitric oxide synthase (NOS). In contrast, chondrocytes treated with LPS or IL-1 beta synthesised nitrite in a dose-related manner. Inhibitors of NOS suppressed nitrite production to below the basal levels of release of unstimulated cells. Dexamethasone, an inhibitor of induction of the inducible isoform of NOS (iNOS), reduced nitrite synthesis by LPS-stimulated chondrocytes. Western blot analysis revealed expression, in response to LPS, of protein in the same molecular weight range as iNOS identified in other species. This work demonstrates that equine chondrocytes have the capacity to synthesise NO, although its exact roles in cartilage metabolism have yet to be determined.
Publication Date: 1997-03-01 PubMed ID: 9104557DOI: 10.1111/j.2042-3306.1997.tb01649.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article explores the ability of horse joint cells to produce nitric oxide, a potential mediator of joint damage, in response to two arthritis-causing molecules, lipopolysaccharide and interleukin-1 beta.
Introduction and Objectives
- The research study aimed to investigate the production of Nitric Oxide (NO) by cultured equine articular chondrocytes. NO has been identified as a potential mediator of articular cartilage damage and an inhibitor of cartilage matrix neosynthesis in several species.
- The investigation focused on the response to two arthritogenic (arthritis-causing) molecules, lipopolysaccharide (LPS) and interleukin-1 beta (IL-1 beta).
Methodology
- In the experiment, equine chondrocytes were cultured and treated with LPS and IL-1 beta. Their ability to synthesise nitrite, a NO metabolite, was measured and compared to synoviocytes stimulated with LPS.
- The researchers also examined the influence of Nitric Oxide Synthase (NOS) inhibitors on nitrite production by the chondrocytes and synoviocytes.
Results
- The researchers found that synoviocytes exhibited a low basal level of NO synthesis that was not significantly increased nor decreased by exposure to LPS. Basal NO synthesis by synoviocytes was not significantly reduced by competitive inhibitors of NOS.
- In contrast, chondrocytes treated with LPS or IL-1 beta synthesised nitrite in a dose-related manner.
- NOS inhibitors suppressed nitrite production to below the basal levels of unstimulated cells.
Conclusion
- The findings reveal that equine chondrocytes have the capacity to synthesise nitric oxide, especially in response to LPS and IL-1 beta.
- However, the exact roles of NO in horse cartilage metabolism remain unclear and warrant further study.
Cite This Article
APA
Frean SP, Bryant CE, Fröling IL, Elliott J, Lees P.
(1997).
Nitric oxide production by equine articular cells in vitro.
Equine Vet J, 29(2), 98-102.
https://doi.org/10.1111/j.2042-3306.1997.tb01649.x Publication
Researcher Affiliations
- Department of Veterinary Basic Sciences, Royal Veterinary College, North Mymms, Herts, UK.
MeSH Terms
- Animals
- Blotting, Western / methods
- Blotting, Western / veterinary
- Cartilage, Articular / cytology
- Cartilage, Articular / drug effects
- Cartilage, Articular / metabolism
- Cells, Cultured
- Dexamethasone / pharmacology
- Dose-Response Relationship, Drug
- Glucocorticoids / pharmacology
- Horses / metabolism
- Interleukin-1 / pharmacology
- Isomerism
- Lipopolysaccharides / pharmacology
- Nitric Oxide / biosynthesis
- Nitric Oxide Synthase / metabolism
- Nitrites / metabolism
- Synovial Membrane / cytology
- Synovial Membrane / drug effects
- Synovial Membrane / metabolism
- Time Factors
Grant Funding
- Wellcome Trust
Citations
This article has been cited 2 times.- Li D, Liu Y, Li Y, Lv Y, Pei X, Guo D. Significance of nitric oxide concentration in plasma and uterine secretes with puerperal endometritis in dairy cows. Vet Res Commun 2010 Apr;34(4):315-21.
- Tung JT, Fenton JI, Arnold C, Alexander L, Yuzbasiyan-Gurkan V, Venta PJ, Peters TL, Orth MW, Richardson DW, Caron JP. Recombinant equine interleukin-1beta induces putative mediators of articular cartilage degradation in equine chondrocytes. Can J Vet Res 2002 Jan;66(1):19-25.
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