Normal reference intervals and the effects of sample handling on dynamic viscoelastic coagulometry (Sonoclot) in healthy adult horses.
Abstract: To determine reference intervals and the effect of sample agitation and rest time on Sonoclot analysis in healthy adult horses. Methods: Original prospective study. Methods: University veterinary medical teaching hospital. Methods: Sixty healthy adult horses. Methods: Blood was collected for assessment of complete blood count, serum biochemical analysis, and Sonoclot analysis. Results: Horses were determined to be healthy based upon physical examination, CBC, and serum biochemistry analysis. Blood was analyzed in a glass bead-containing cuvette using the Sienco Sonoclot analyzer following 2 rest periods (30 mins and 240 min) and with 2 sample handling interventions (agitated and nonagitated), to obtain values for clot rate, time-to-peak, activated clotting time, and platelet function. This study failed to detect a significant difference when a rest time of 30 minutes was compared with 240 minutes, but based on wide limits of agreement the 2 rest times were not considered interchangeable. Agitation at both rest times significantly affected all Sonoclot analyses leading to changes indicative of hypercoagulability. Conclusions: Sample agitation and rest time should be taken into consideration when developing preanalytical guidelines for Sonoclot analysis in horses. Calculated reference intervals were relatively wide. Further research is needed to evaluate the clinical utility of Sonoclot analysis in horses.
© Veterinary Emergency and Critical Care Society 2017.
Publication Date: 2017-12-15 PubMed ID: 29244257DOI: 10.1111/vec.12687Google Scholar: Lookup
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- Journal Article
Summary
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This study investigates the standard parameters and the impact of sample handling on a blood clotting test known as Sonoclot, performed on healthy adult horses.
Objective of the study
- The study’s primary goal was to establish reference intervals (normal ranges) and investigate the impact of two variables, agitation and rest time, on Sonoclot analysis in horses. The Sonoclot analyzer is a device that assesses blood clotting function.
Methods
- The research was conducted at a university veterinary medical teaching hospital, involving sixty healthy adult horses. The horses’ health was confirmed through physical examination, complete blood count (CBC), and serum biochemistry analysis.
- Blood from the horses was collected and assessed using a range of tests, including CBC, serum biochemical analysis, and the Sonoclot analyzer. The Sonoclot test was designed to yield values for clot rate, time-to-peak, activated clotting time, and platelet function, thus providing a comprehensive profile of the blood’s clotting ability.
- The Sonoclot test was conducted following two different rest periods (30 minutes and 240 minutes) and under two sample handling conditions – agitated and non-agitated.
Results
- The study found no significant difference in Sonoclot outcomes when comparing a rest time of 30 minutes to 240 minutes. However, due to the wide limits of agreement, the two rest periods were not considered interchangeable.
- Agitation during both rest times significantly influenced the Sonoclot analyses, causing changes representative of an increased propensity for blood to clot (hypercoagulability).
Conclusions and Recommendations
- The study concluded that sample agitation and rest time should be considered when setting preanalytical guidelines for Sonoclot analysis in horses, as these factors can significantly impact the results.
- The computed reference intervals from this study were broadly defined, indicating a wide range of normal values for healthy horses.
- Further research is advised to assess the clinical application of Sonoclot analysis in horses and potentially refine the reference intervals.
Cite This Article
APA
Jamieson CA, Hanzlicek AS, Payton ME, Holbrook TC.
(2017).
Normal reference intervals and the effects of sample handling on dynamic viscoelastic coagulometry (Sonoclot) in healthy adult horses.
J Vet Emerg Crit Care (San Antonio), 28(1), 39-44.
https://doi.org/10.1111/vec.12687 Publication
Researcher Affiliations
- Department of Veterinary Clinical Sciences, Oklahoma State University, Stillwater, OK 74078.
- Department of Veterinary Clinical Sciences, Oklahoma State University, Stillwater, OK 74078.
- Department of Statistics, Oklahoma State University, Stillwater, OK 74078.
- Department of Veterinary Clinical Sciences, Oklahoma State University, Stillwater, OK 74078.
MeSH Terms
- Animals
- Blood Coagulation Tests / veterinary
- Female
- Hemostasis / physiology
- Horses / blood
- Horses / physiology
- Male
- Platelet Function Tests
- Prospective Studies
- Reference Values
- Specimen Handling / veterinary
- Thrombophilia
Citations
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