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Home / Equine Research Bank / Equine Vet J / Novel equid papillomavirus from domestic donkey.
Equine veterinary journal2023; doi: 10.1111/evj.13957

Novel equid papillomavirus from domestic donkey.

Abstract: Papillomaviruses can be of great medical importance as they infect humans and animals such as Equus species, other livestock and pets. They are responsible for several papillomas and benign tumours in their host. Objective: To describe a novel equid papillomavirus detected in oral swab samples collected from donkeys (Equus asinus) found on the Northwest plateau of China. Methods: Cross-sectional. Methods: Swab samples collected from the oral mucosa of 32 donkeys in the Gansu Province of China, were subjected to viral metagenomic analysis to detect the presence of Papillomavirus. After de novo assembly, a novel papillomavirus genome designated as Equus asinus papillomavirus 3 (EaPV3) was identified in the studied samples. Additional bioinformatic analysis of the assembled genome was done using the Geneious prime software (version 2022.0.2). Results: The complete circular genome of EaPV3 is 7430 bp in length with a GC content of 50.8%. The genome was predicted to contain five ORFs coding for three early proteins (E7, E1, and E2) and two late proteins (L1 and L2). Phylogenic analysis of the nucleotide sequences of the concatenated amino acid sequences of the E1E2L1L2 genes revealed that EaPV3 is most closely related to Equus asinus papillomavirus 1 (EaPV1). The genome analysis of EaPV3 revealed similar genome organisation with other equine papillomavirus and the presence of E7 papillomavirus oncoprotein. Conclusions: Since there were no warts in the oral cavity of the donkeys in this study, and no biopsy samples taken, we are unable to conclusively link the novel virus to any clinical condition in the donkeys. Conclusions: The Comparative characterisation of EaPV3 and its closest relatives, as well as phylogenetic analysis demonstrated that it is a novel virus specie that clusters within the Dyochipapilloma PV genus. Unassigned: O papilomavírus pode apresentar uma importância médica significante por poder infectar humanos e animais, incluindo animais da espécie Equus, animais de produção e animais domésticos. Esse vírus é responsável por vários papilomas e tumores benignos em seus hospedeiros. Objective: Descrever um novo papilomavírus detectado em amostras de swab oral coletados de Equus asinus no Noroeste da China. Unassigned: Estudo transversal. Methods: Amostras de swab coletadas da mucosa oral de 32 jumentos da província de Gansu na China foram submetidos para análise metagenômica viral para detectar a presença de papilomavírus. Após a montagem de novo, um novo papilomavírus, denominado Equus asinus papilomavírus 3 (EaPV3), foi identificado nas amostras estudadas. Análise bioinformática adicional foi realizada para montar o genoma desse vírus usando o Geneious prime software (version 2022.0.2). Results: O genoma circular completo do EaPV3 possui 7.430 bp, e apresenta 50.8% de conteúdo GC. Foi previsto que o genoma possui cinco codificações ORFs para três proteínas precoces (E7, E1 e E2) e duas proteínas retardas (L1 2 L2). Análise filogenética da sequência de nucleotídeos dos genes E1E2L1L2 dos amino ácidos concatenados revelaram que o EaPV3 é bastante similar aos EaPV1. A análise genômica do EaPV3 revelou uma organização genômica similar com outros papilomavírus equinos e a presença da oncoproteína E7. PRINCIPAIS LIMITAÇÕES: Como não havia nenhuma verruga na cavidade oral dos jumentos investigados neste estudo, e nenhuma biópsia foi realizada, não é possível concluir uma associação entre este novo vírus e qualquer condição clínica em jumentos. CONCLUSÕES: A caracterização comparativa do EaPV3 com outros vírus similares e a análise filogenética demonstraram que este é um novo vírus que assemelha-se com os papilomavírus do gene Dyochipapilloma.
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Publication Date: 2023-05-28 PubMed ID: 37246448DOI: 10.1111/evj.13957Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research describes the identification and analysis of a new type of papillomavirus found in donkeys in China. However, no definitive link was found between the virus and any specific health conditions in the donkeys.

Research Objective

The objective of this study was to identify and describe a novel equid papillomavirus found in oral swab samples collected from donkeys in the Gansu Province of China. Papillomaviruses can cause several types of papillomas and benign tumours in their hosts, including humans, livestock and pets.

Methods Used

  • The researchers collected oral swab samples from 32 donkeys and subjected them to viral metagenomic analysis to detect the presence of Papillomavirus.
  • Following de novo assembly, a new papillomavirus genome was detected, which they named Equus asinus papillomavirus 3 (EaPV3).
  • Additional analysis of the sequenced genome was done using Geneious prime software.

Research Findings

  • The complete EaPV3 genome is 7,430 base pairs long, with a GC content of 50.8%.
  • The genome was found to code for three early proteins (E7, E1, and E2) and two late proteins (L1 and L2).
  • The nucleotide sequences of the E1E2L1L2 genes showed that EaPV3 is most closely related to Equus asinus papillomavirus 1 (EaPV1).
  • The researchers also discovered the presence of the E7 papillomavirus oncoprotein in the EaPV3 genome.

Conclusions

  • Despite the discovery of EaPV3, the researchers were unable to conclusively link the virus to any specific clinical condition in the donkeys, primarily because no warts were found in the animals’ oral cavities and no biopsy samples were taken.
  • Upon comparison with other similar viruses and through phylogenetic analysis, EaPV3 was found to be a novel species of virus that clusters within the Dyochipapilloma PV genus.

Cite This Article

APA
Ikechukwu CK, Qin K, Zhang H, Pan J, Zhang W. (2023). Novel equid papillomavirus from domestic donkey. Equine Vet J. https://doi.org/10.1111/evj.13957

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English

Researcher Affiliations

Ikechukwu, Chukwudozie Kingsley
  • Department of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China.
  • Department of Microbiology, University of Nigeria, Nsukka, Nigeria.
Qin, Kailin
  • Department of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China.
Zhang, Han
  • Department of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China.
Pan, Jiamin
  • Department of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China.
Zhang, Wen
  • Department of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China.

Grant Funding

  • National Key Research and Development Programs of China No. 2022YFC2603801 and Funding for Kunlun Talented People of Qinghai Province, High-end Innovation and Entrepreneurship Talents-leading Talents No. 202208170046

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