O2 delivery at VO2max and oxidative capacity in muscles of standardbred horses.
Abstract: The purpose of this study was to describe the relationships between 16 physiological, biochemical, and morphological variables presumed to relate to the oxidative capacity in quadriceps muscles or muscle parts in Standardbred horses. The variables included O2 delivery (blood flow) and mean capillary transit time (MTT) during treadmill locomotion at whole animal maximal O2 consumption (VO2max, 134 +/- 2 ml.min-1 x kg-1), capillary density and capillary-to-fiber ratio, myoglobin concentration, oxidative enzyme activities, glycolytic enzyme activities, fiber type populations, and fiber size. These components of muscle metabolic capacity were found to be interrelated to varying degrees using correlation matrix analysis, with lactate dehydrogenase activity showing the most significant correlations (n = 14) with other variables. Most of the "oxidative" variables occurred in the highest quantities in the deepest muscle of the group (vastus intermedius) and in the deepest parts of the other quadriceps muscles where the highest proportions of type I fibers were localized. The highest blood flow measured with microspheres in the muscle group during exercise was in vastus intermedius muscle (145 ml.min-1 x 100 g-1), and the lowest was in the superficial part of rectus femoris muscle (32 ml.min-1 x 100 g-1). Average muscle blood flow during exercise at whole animal VO2max was 116 ml.min-1 x 100 g-1. Because skeletal muscle comprised 43% of total body mass (453 +/- 34 kg), total muscle blood flow was estimated at 226 l/min, which was approximately 78% of total cardiac output (288 l/min).(ABSTRACT TRUNCATED AT 250 WORDS)
Publication Date: 1992-12-01 PubMed ID: 1337073DOI: 10.1152/jappl.1992.73.6.2274Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- Non-P.H.S.
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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The research article examines the relationship between multiple physiological, biochemical, and morphological factors theorized to be involved in the oxidative capacity of Standardbred horses’ quadriceps muscles. Notably, these factors include oxygen delivery, capillary density, and muscle fiber type distribution.
Understanding Oxidative Capacity and Variables
- The study focuses on understanding the complex network of factors that correlate with and potentially impact the oxidative capacity in quadriceps muscle sections of Standardbred horses.
- The researchers considered 16 key variables, including oxygen delivery, the mean capillary transit time during treadmill exercise at the animal’s maximal oxygen consumption,myoglobin concentration, and various enzyme activities.
- The properties and activities of various enzyme types, fiber populations, and fiber sizes also play a role in this investigation.
Correlations Found
- The researchers found that these aspects of muscle metabolic capacity were interconnected to varying extents, as shown by correlation matrix analysis.
- Among all the variables, lactate dehydrogenase activity exhibited the most significant correlations with other aspects.
Relevance of Muscle Depth and Fiber Type
- The study revealed that the highest quantities of “oxidative” variables were present in the deeper muscles of the quadriceps group (vastus intermedius), as well as in the deeper sections of other quadriceps muscles where type I fibers were predominantly located.
- This suggests the vital role of muscle depth and fiber type in determining oxidative capacity.
Importance of Blood Flow
- In terms of blood flow, highest rates were recorded in the vastus intermedius muscle during exercise, while the lowest were detected in the superficial part of the rectus femoris muscle.
- This indicates how blood flow, and therefore presumably oxygen delivery, can significantly vary within different regions of the same muscle group.
- The research also highlighted the importance of blood flow in terms of cardiac output, with the muscles taking approximately 78% of total cardiac output, underlining the significant cardiovascular demands of equine locomotion.
Cite This Article
APA
Armstrong RB, Essén-Gustavsson B, Hoppeler H, Jones JH, Kayar SR, Laughlin MH, Lindholm A, Longworth KE, Taylor CR, Weibel ER.
(1992).
O2 delivery at VO2max and oxidative capacity in muscles of standardbred horses.
J Appl Physiol (1985), 73(6), 2274-2282.
https://doi.org/10.1152/jappl.1992.73.6.2274 Publication
Researcher Affiliations
- Muscle Biology Laboratory, University of Georgia, Athens 30602.
MeSH Terms
- Animals
- Capillaries / physiology
- Cardiac Output / physiology
- Electron Transport Complex IV / metabolism
- Female
- Glycolysis / physiology
- Horses
- L-Lactate Dehydrogenase / metabolism
- Male
- Microspheres
- Muscles / cytology
- Muscles / enzymology
- Muscles / metabolism
- Myoglobin / metabolism
- Oxidation-Reduction
- Oxygen Consumption / physiology
Grant Funding
- AM-37098 / NIADDK NIH HHS
Citations
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- Schippers MP, Ramirez O, Arana M, McClelland GB. Increased Reliance on Carbohydrates for Aerobic Exercise in Highland Andean Leaf-Eared Mice, but Not in Highland Lima Leaf-Eared Mice. Metabolites 2021 Oct 29;11(11).
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