Occurrence of an unusual phosphorylated N-acetyllactosamine in horse colostrum.
Abstract: The colostrum of horses (thoroughbreds) was extracted and fractionated to yield Gal(beta1-4)GlcNAcalpha1-phosphate, which has not previously been detected in any mammalian milk or colostrum, as well as Neu5Ac(alpha2-3)Gal(beta1-4)Glc. The structures of these saccharides were established by NMR spectroscopy and MALDI-TOF mass spectrometry.
Publication Date: 2001-05-09 PubMed ID: 11342248DOI: 10.1016/s0304-4165(00)00148-3Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research investigates a unique form of phosphorylated N-acetyllactosamine found in horse colostrum that hasn’t been previously identified in any other mammalian milk or colostrum.
Introduction
- The study focuses on horse colostrum – the first form of milk produced by mammalian mothers immediately following birth. Particularly, the objective was to discover unique components within the colostrum of thoroughbred horses.
- The substance of interest here is a complex sugar, or saccharide, named Gal(beta1-4)GlcNAcalpha1-phosphate. This particular form of phosphorated N-acetyllactosamine was isolated during the study and has not been previously discovered in any other type of mammalian colostrum or milk.
Methods
- The horse colostrum was extracted and fractionated, a process of dividing or categorizing, in an effort to isolate the unique saccharide.
- Two saccharides were successfully isolated – Gal(beta1-4)GlcNAcalpha1-phosphate and Neu5Ac(alpha2-3)Gal(beta1-4)Glc. The former being of particular interest due to its previously unseen occurrence.
Analysis and Findings
- Once the two saccharides were isolated, they were thoroughly analyzed using Nuclear Magnetic Resonance (NMR) spectroscopy and Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF) mass spectrometry.
- NMR spectroscopy is an analytical technique to determine chemical structure of molecules. It employs powerful magnetic fields to establish the physical and chemical properties of atoms or the molecules in which they are contained.
- MALDI-TOF mass spectrometry is a method of identifying unknown compounds and studying biological macromolecules. In this case, it aided in confirming the structure of the isolated saccharides.
Conclusion
- The study confirmed the existence of Gal(beta1-4)GlcNAcalpha1-phosphate in horse colostrum. This discovery marks for the first time this type of phosphorylated N-acetyllactosamine has been detected in mammalian milk or colostrum.
- The findings from the research could have potential implications in understanding horse biology and potentially in the therapeutic and nutritional properties of horse colostrum.
Cite This Article
APA
Nakamura T, Amikawa S, Harada T, Saito T, Arai I, Urashima T.
(2001).
Occurrence of an unusual phosphorylated N-acetyllactosamine in horse colostrum.
Biochim Biophys Acta, 1525(1-2), 13-18.
https://doi.org/10.1016/s0304-4165(00)00148-3 Publication
Researcher Affiliations
- Department of Bioresource, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan. nakamura@obihiro.ac.jp
MeSH Terms
- Amino Sugars / chemistry
- Amino Sugars / isolation & purification
- Animals
- Carbohydrate Sequence
- Colostrum / chemistry
- Female
- Horses
- Magnetic Resonance Spectroscopy
- Molecular Sequence Data
- Oligosaccharides / chemistry
- Oligosaccharides / isolation & purification
- Phosphorylation
- Pregnancy
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Citations
This article has been cited 6 times.- Durham SD, Wei Z, Lemay DG, Lange MC, Barile D. Creation of a milk oligosaccharide database, MilkOligoDB, reveals common structural motifs and extensive diversity across mammals.. Sci Rep 2023 Jun 26;13(1):10345.
- Pyles MB, Brock K, Schendel RR, Lawrence LM. Improved methods for mare milk analysis: Extraction and quantification of mare milk carbohydrates and assessment of FTIR-based macronutrient quantification.. Front Nutr 2023;10:1066463.
- Karav S, Salcedo J, Frese SA, Barile D. Thoroughbred mare's milk exhibits a unique and diverse free oligosaccharide profile.. FEBS Open Bio 2018 Aug;8(8):1219-1229.
- Kirmiz N, Robinson RC, Shah IM, Barile D, Mills DA. Milk Glycans and Their Interaction with the Infant-Gut Microbiota.. Annu Rev Food Sci Technol 2018 Mar 25;9:429-450.
- Sasaki M, Nakamura T, Hirayama K, Fukuda K, Saito T, Urashima T, Asakuma S. Characterization of two novel sialyl N-acetyllactosaminyl nucleotides separated from ovine colostrum.. Glycoconj J 2016 Oct;33(5):789-96.
- Urashima T, Saito T, Nakamura T, Messer M. Oligosaccharides of milk and colostrum in non-human mammals.. Glycoconj J 2001 May;18(5):357-71.
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