Optimising Stallion Semen Cryopreservation: Preliminary Insights Into Pre-Centrifugation Extender Effects.

1. Aurich, C. 2005. “Factors Affecting the Plasma Membrane Function of Cooled‐Stored Stallion Spermatozoa.” Animal Reproduction Science 89, no. 1–4: 65–75.
   pubmed: 16081230
2. Aurich, J. , Kuhl J., Tichy A., and Aurich C.. 2020. “Efficiency of Semen Cryopreservation in Stallions.” Animals 10, no. 6: 1033.
   pmc: PMC7341245pubmed: 32545785
3. Brinsko, S. P. , Blanchard T. L., Rigby S. L., Love C. C., and Varner D. D.. 2003. “Effects of Dead Spermatozoa on Motion Characteristics and Membrane Integrity of Live Spermatozoa in Fresh and Cooled‐Stored Equine Semen.” Theriogenology 59, no. 3–4: 735–742.
   pubmed: 12517377
4. Campos, G. A. , Garcia V. F. C., Freitas‐Dell'Aqua C. P., et al. 2020. “Sodium Caseinate and Cholesterol Improve Bad Cooler Stallion Fertility.” Journal of Equine Veterinary Science 93: 103201. 10.1016/j.jevs.2020.103201.
   doi: 10.1016/j.jevs.2020.103201pubmed: 32972672google scholar: lookup
5. Carneiro, J. A. M. , Canisso I. F., Bandeira R. S., et al. 2018. “Effects of Coenzyme Q10 on Semen Cryopreservation of Stallions Classified as Having Good or Bad Semen Freezing Ability.” Animal Reproduction Science 192: 107–118. 10.1016/j.anireprosci.2018.02.020.
   doi: 10.1016/j.anireprosci.2018.02.020pubmed: 29502896google scholar: lookup
6. Colégio Brasileiro de Reprodução Animal (CBRA) . 2013. Manual para exame andrológico e avaliação de sêmen animal. 3rd ed. CBRA.
7. El‐Amrawi, G. A. , Metwally K. K., and Ibrahim O.. 2021. “Influences of Different Extenders on Quality and Fertilizing Capacity of Stallion Semen.” Alexandria Journal of Veterinary Sciences 71: 56–61.
8. Foster, M. L. , Varner D. D., Hinrichs K., et al. 2011. “Agreement Between Measures of Total Motility and Membrane Integrity in Stallion Sperm.” Theriogenology 75: 1499–1505. 10.1016/j.theriogenology.2010.12.011.
   doi: 10.1016/j.theriogenology.2010.12.011pubmed: 21295826google scholar: lookup
9. Gil, M. A. , Hernandez M., Roca J., et al. 2010. “Pentoxifylline Added to Freezing or Post‐Thaw Extenders Does Not Improve the Survival or In Vitro Fertilising Capacity of Boar Spermatozoa.” Reproduction 139: 557–564. 10.1530/REP-09-0274.
   doi: 10.1530/REP-09-0274pubmed: 20032214google scholar: lookup
10. Gradil, C. M. , and Ball B. A.. 2000. “The Use of Pentoxifylline to Improve Motility of Cryopreserved Equine Spermatozoa.” Theriogenology 54, no. 7: 1041–1047. 10.1016/S0093-691X(00)00412-X.
    doi: 10.1016/S0093-691X(00)00412-Xpubmed: 11131322google scholar: lookup
11. Guasti, P. N. , Monteiro G. A., Maziero R. R. D., et al. 2017. “Pentoxifylline Effects on Capacitation and Fertility of Stallion Epididymal Sperm.” Animal Reproduction Science 179: 27–34. 10.1016/j.anireprosci.2017.01.013.
    doi: 10.1016/j.anireprosci.2017.01.013pubmed: 28238445google scholar: lookup
12. Harrison, R. A. P. , and Vickers S. E.. 1990. “Use of Fluorescent Probes to Assess Membrane Integrity in Mammalian Spermatozoa.” Reproduction 88: 343–352. 10.1530/jrf.0.0880343.
    doi: 10.1530/jrf.0.0880343pubmed: 1690300google scholar: lookup
13. Hartwig, F. P. , Lisboa F. P., Hartwig F. P., et al. 2014. “Use of Cholesterol‐Loaded Cyclodextrin: An Alternative for Bad Cooler Stallions.” Theriogenology 81: 340–346. 10.1016/j.theriogenology.2013.10.003.
    doi: 10.1016/j.theriogenology.2013.10.003pubmed: 24182738google scholar: lookup
14. Larentis, G. R. , Camozzato G. C., Bastos H. B., Gregory R. M., and Mattos R. C.. 2018. “Equine Sperm Selection by Synthetic Membrane Filter.” Journal of Equine Veterinary Science 63: 69–73.
15. Negri, P. , Grechi E., Tomasi A., Fabbri E., and Capuzzo A.. 1996. “Effectiveness of Pentoxifylline in Semen Preparation for Intrauterine Insemination.” Human Reproduction 11: 1236–1239. 10.1093/oxfordjournals.humrep.a019363.
    doi: 10.1093/oxfordjournals.humrep.a019363pubmed: 8671431google scholar: lookup
16. Papa, F. O. , Melo C. M., Fioratti E. G., Dell'Aqua J. A. Jr., Zahn F. S., and Alvarenga M. A.. 2008. “Freezing of Stallion Epididymal Sperm.” Animal Reproduction Science 107: 293–301. 10.1016/j.anireprosci.2008.05.003.
    doi: 10.1016/j.anireprosci.2008.05.003pubmed: 18556154google scholar: lookup
17. Peña, F. J. , and Gibb Z.. 2022. “Oxidative Stress and Reproductive Function: Oxidative Stress and the Long‐Term Storage of Horse Spermatozoa.” Reproduction 164, no. 6: F135–F144.
    pubmed: 36255038
18. Peter, A. T. , Brito L., Althouse G., et al. 2021. “Andrology Laboratory Review: Evaluation of Sperm Motility.” Clinical Theriogenology 13, no. 1: 24–36.
    pubmed: 27045626
19. Sharafi, M. , Borghei‐Rad S. M., Hezavehei M., Shahverdi A., and Benson J. D.. 2022. “Cryopreservation of Semen in Domestic Animals: A Review of Current Challenges, Applications, and Prospective Strategies.” Animals 12, no. 23: 3271.
    pmc: PMC9739224pubmed: 36496792
20. Spizziri, B. E. , Fox M. H., Bruemmer J. E., Squires E. L., and Graham J. K.. 2010. “Cholesterol‐Loaded‐Cyclodextrins and Fertility Potential of Stallions Spermatozoa.” Animal Reproduction Science 118: 255–264. 10.1016/j.anireprosci.2009.08.001.
    doi: 10.1016/j.anireprosci.2009.08.001pubmed: 19762177google scholar: lookup
21. Stephens, T. D. , Brooks R. M., Carrington J. L., et al. 2013. “Effects of Pentoxifylline, Caffeine, and Taurine on Post‐Thaw Motility and Longevity of Equine Frozen Semen.” Journal of Equine Veterinary Science 33: 615–621. 10.1016/j.jevs.2012.10.004.
    doi: 10.1016/j.jevs.2012.10.004google scholar: lookup
22. Zahn, F. S. , Papa F. O., and Dell'Aqua Júnior J. A.. 2002. “Cholesterol Incorporation on Equine Sperm Membrane: Effects on Post‐Thaw Sperm Parameters and Fertility.” Theriogenology 58, no. 2: 237–240.
23. Zanlorenzi‐Silva, L. P. , Freitas‐Dell'Aqua C. P., Baby M. E. A., et al. 2025. “The Protective Capacity of Different Diluents on Stallion Sperm During Storage in a Passive Refrigeration System ‘Botuflex®’ for 24 h at 5°C Before Freezing.” Journal of Equine Veterinary Science 145: 105337.

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