Overexpression of histidine-rich calcium binding protein in equine ventricular myocardium.
Abstract: Histidine-rich calcium binding protein (HRC) is a high capacity, low affinity Ca(2+) binding protein, specifically expressed in striated muscles of mammals. In rabbit skeletal and cardiac muscles, HRC binds to sarcoplasmic reticulum (SR) membranes via triadin, a junctional SR protein. Recently, a potential role in heart failure and arrhythmogenesis has been assigned to HRC due to its activity as regulator of SR Ca(2+) uptake and Ca(2+) release. HRC might play a particularly relevant role in the equine heart, given its slower resting heart rate (20-35 beats/min) and longer action potential duration (APD) (0.6-1.0 s) than are found in other mammals. The results from this study showed for the first time direct evidence that HRC protein in equine cardiac muscle was expressed in association with the SR membranes and that HRC transcriptional activity was three times higher in the ventricles compared to the atria. The predominance of HRC mRNA up-regulation in ventricular myocardium was specific to the horse heart, since a more even distribution between atria and ventricles was found in animals of similar body size or species, such as cattle or domestic donkeys.
Copyright © 2011 Elsevier Ltd. All rights reserved.
Publication Date: 2011-10-29 PubMed ID: 22040806DOI: 10.1016/j.tvjl.2011.09.022Google Scholar: Lookup
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- Journal Article
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Summary
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The research article analyzes the high presence of Histidine-rich calcium binding protein (HRC) in the ventricles of horses’ hearts, possibly due to their unique heart rates and action potential duration compared to other mammals.
Histidine-rich Calcium Binding Protein (HRC) – An Overview
- Histidine-rich calcium binding protein (HRC) is a protein that binds to calcium.
- It exhibits a high capacity for binding calcium but does so with a low affinity.
- HRC is found specifically in striated muscles of mammals. Striated muscles are the skeletal muscles that are attached to the bones and are involved in voluntary movement.
- In previous research, HRC has been found to bind to the sarcoplasmic reticulum (SR) membranes in both skeletal and cardiac muscles of rabbits. The sarcoplasmic reticulum is a type of endoplasmic reticulum found in muscle cells, responsible for storing and releasing calcium ions (Ca[2+]), which are essential for muscle contractions.
- Trecently, HRC has been suggested to potentially play a role in heart failure and arrhythmogenesis (the formation of irregular heartbeats) due to its role in regulating SR calcium uptake and release.
The Significance of HRC in Equine Heart
- The research suggests that HRC might have a particularly significant role in horses’ hearts, which have a slower resting heart rate (20-35 beats/min) and longer action potential duration (0.6-1.0 s) than other mammals.
- During the testing phase, the study showed that HRC protein was expressed in the SR membranes inside the cardiac muscles of horses. This is the first time such evidence has been found.
- The study also discovered that HRC’s transcriptional activity was three times higher in the ventricles compared to the atria of a horse’s heart.
Comparative Analysis of HRC in Different Species
- The higher presence of HRC mRNA (a molecule that carries information from the DNA to the site of protein synthesis) in the ventricle as compared to the atrium is unique to horse hearts.
- Animals of similar body size or from similar species, such as cattle or domestic donkeys, showed a more evenly distributed presence of HRC mRNA between their atria and ventricles.
Cite This Article
APA
Sacchetto R, Sharova E, Patruno M, Maccatrozzo L, Damiani E, Mascarello F.
(2011).
Overexpression of histidine-rich calcium binding protein in equine ventricular myocardium.
Vet J, 193(1), 157-161.
https://doi.org/10.1016/j.tvjl.2011.09.022 Publication
Researcher Affiliations
- Department of Experimental Veterinary Sciences, University of Padova, 35020 Legnaro Padova, Italy. roberta.sacchetto@unipd.it
MeSH Terms
- Action Potentials
- Animals
- Calcium / metabolism
- Calcium-Binding Proteins / metabolism
- Cattle / physiology
- Equidae / physiology
- Heart Atria / metabolism
- Heart Ventricles / metabolism
- Horses / physiology
- Myocardial Contraction
- Myocardium / metabolism
- Proteins / metabolism
- RNA, Messenger / metabolism
- Real-Time Polymerase Chain Reaction
- Sarcoplasmic Reticulum / metabolism
- Sequence Alignment
- Sequence Analysis, Protein
- Time Factors
Citations
This article has been cited 2 times.- Autry JM, Karim CB, Cocco M, Carlson SF, Thomas DD, Valberg SJ. Purification of sarcoplasmic reticulum vesicles from horse gluteal muscle. Anal Biochem 2020 Dec 1;610:113965.
- Amioka M, Nakano Y, Ochi H, Onohara Y, Sairaku A, Tokuyama T, Motoda C, Matsumura H, Tomomori S, Hironobe N, Okubo Y, Okamura S, Chayama K, Kihara Y. Ser96Ala genetic variant of the human histidine-rich calcium-binding protein is a genetic predictor of recurrence after catheter ablation in patients with paroxysmal atrial fibrillation. PLoS One 2019;14(3):e0213208.
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