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Home / Equine Research Bank / J Vet Intern Med / Oxidant-antioxidant status in the blood of horses with sympt...
Journal of veterinary internal medicine2014; 28(6); 1845-1852; doi: 10.1111/jvim.12452

Oxidant-antioxidant status in the blood of horses with symptomatic recurrent airway obstruction (RAO).

Abstract: Systemic oxidative stress in horses with recurrent airway obstruction (RAO) is poorly characterized. Objective: The goal of this study was to investigate whether equine RAO is associated with systemic disturbances in the oxidant-antioxidant equilibrium. Methods: Seven healthy horses and 7 horses with symptomatic RAO. Methods: A prospective study. Healthy and RAO-affected horses were exposed to a 48-hour challenge with moldy hay and straw to induce clinical exacerbation of RAO. Venous blood was collected and the activities of the superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in equine erythrocyte lysates were measured. The concentration of thiobarbituric acid-reactive substances (TBARSs) was assessed both in erythrocyte lysates and in plasma. Results: A significant increase in the activities of GPx and SOD was detected in RAO-affected horses compared with the control animals. There was no significant difference between groups in terms of the erythrocyte lysate activities of CAT, GR, or TBARs or the plasma concentration of TBARs. Conclusions: Our results support the hypothesis that RAO in horses is associated with systemic oxidative stress. Future studies are needed to assess whether horses suffering from RAO can benefit from antioxidant supplementation.
Copyright © 2014 by the American College of Veterinary Internal Medicine.
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Publication Date: 2014-09-17 PubMed ID: 25231643PubMed Central: PMC4895620DOI: 10.1111/jvim.12452Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates exactly how recurrent airway obstruction (RAO) in horses can affect their oxidant-antioxidant equilibrium. The results suggest that RAO could be associated with systemic oxidative stress.

Objective of the study

  • The main goal of the study was to investigate if an imbalance in the oxidant-antioxidant equilibrium could contribute to the development of RAO in horses.

Methodology of the study

  • The researchers involved 14 horses in their study. Seven of these horses were healthy, while the remaining seven had symptomatic RAO.
  • All the horses were exposed to moldy hay and straw over a period of 48 hours to induce RAO.
  • The scientists collected venous blood from each horse after this period and measured the activities of four specific enzymes (superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase) present in the equine erythrocyte lysates. These enzymes are directly involved in antioxidant defense.
  • The researchers also measured the concentration of thiobarbituric acid-reactive substances in both erythrocyte lysates and plasma. This substance is a byproduct of lipid peroxidation and is often used as a marker for oxidative stress.

Outcome of the study

  • The results revealed that there was a significant increase in the activities of glutathione peroxidase and superoxide dismutase in horses affected by RAO compared to the healthy horses.
  • The study, however, did not find any significant differences in the activities of catalase, glutathione reductase, or TBARs between the two groups.

Conclusion

  • The study concluded that RAO in horses could be associated with systemic oxidative stress as indicated by increased enzymatic activity of certain antioxidants in RAO-affected horses.
  • The researchers suggest that future studies should be conducted to see if antioxidant supplementation could benefit horses suffering from RAO.

Cite This Article

APA
Niedzwiedz A, Jaworski Z. (2014). Oxidant-antioxidant status in the blood of horses with symptomatic recurrent airway obstruction (RAO). J Vet Intern Med, 28(6), 1845-1852. https://doi.org/10.1111/jvim.12452

Publication

Journal of veterinary internal medicine
ISSN: 1939-1676
NlmUniqueID: 8708660
Country: United States
Language: English
Volume: 28
Issue: 6
Pages: 1845-1852

Researcher Affiliations

Niedzwiedz, A
  • Department of Internal Diseases with Clinic for Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wrocaw University of Environmental and Life Sciences, Pl. Grunwaldzki 47, 50-366, Wrocaw, Poland.
Jaworski, Z

    MeSH Terms

    • Airway Obstruction / blood
    • Airway Obstruction / metabolism
    • Airway Obstruction / veterinary
    • Animals
    • Bronchoalveolar Lavage Fluid / cytology
    • Case-Control Studies
    • Catalase / blood
    • Female
    • Glutathione Peroxidase / blood
    • Glutathione Reductase / blood
    • Horse Diseases / blood
    • Horse Diseases / metabolism
    • Horses / blood
    • Horses / metabolism
    • Male
    • Oxidation-Reduction
    • Oxidative Stress
    • Prospective Studies
    • Recurrence
    • Superoxide Dismutase / blood

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    Citations

    This article has been cited 6 times.
    1. Simões J, Batista M, Tilley P. The Immune Mechanisms of Severe Equine Asthma-Current Understanding and What Is Missing.. Animals (Basel) 2022 Mar 16;12(6).
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    3. Borowska A, Wolska D, Niedzwiedz A, Borowicz H, Jaworski Z, Siemieniuch M, Szwaczkowski T. Some Genetic and Environmental Effects on Equine Asthma in Polish Konik Horses.. Animals (Basel) 2021 Aug 3;11(8).
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    4. Żak A, Siwińska N, Chełmecka E, Bażanów B, Romuk E, Adams A, Niedźwiedź A, Stygar D. Effects of Advanced Age, Pituitary Pars Intermedia Dysfunction and Insulin Dysregulation on Serum Antioxidant Markers in Horses.. Antioxidants (Basel) 2020 May 21;9(5).
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    5. Bullone M, Lavoie JP. The Contribution of Oxidative Stress and Inflamm-Aging in Human and Equine Asthma.. Int J Mol Sci 2017 Dec 5;18(12).
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    6. Niedzwiedz A, Borowicz H, Januszewska L, Markiewicz-Gorka I, Jaworski Z. Serum 8-hydroxy-2-deoxyguanosine as a marker of DNA oxidative damage in horses with recurrent airway obstruction.. Acta Vet Scand 2016 Jun 7;58(1):38.
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