Oxidative stress markers in Thoroughbred horses after castration surgery under inhalation anesthesia.
Abstract: Oxidative stress has been reported to occur during surgery. It is important to reduce intraoperative oxidative stress to improve the postoperative prognosis. However, there are no reports regarding oxidative stress related to surgery in horses. In the present study, we measured pre and postsurgical diacron-reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP); the oxidative stress index (OSI) was then calculated (OSI=d-ROMs/BAP × 100). d-ROMs were not significantly different between the pre and postsurgical periods. However, BAP significantly decreased after surgery (P=0.02), and OSI significantly increased after surgery (P=0.02). Based on these results, it suggested that castration surgery under inhalation anesthesia decreases the antioxidant potential and causes oxidative stress in horses.
Publication Date: 2016-06-21 PubMed ID: 27330401PubMed Central: PMC4914400DOI: 10.1294/jes.27.77Google Scholar: Lookup
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- Journal Article
Summary
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This research investigates the occurrence of oxidative stress, a harmful condition caused by an imbalance of antioxidants and free radicals, in Thoroughbred horses following castration surgery under inhalation anesthesia. The study observes significant decreases in the horses’ biological antioxidant potential (BAP) – the body’s means of managing free radicals – post-surgery, suggesting the operation might induce oxidative stress.
Research Objective and Methodology
- The primary aim of this study was to examine oxidative stress in Thoroughbred horses due to surgery, specifically focusing on the period following castration surgery conducted under inhalation anesthesia. There has previously been no research focusing on this aspect.
- To explore this, the researchers measured levels of diacron-reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) in the horses both before and after surgery. Levels of d-ROMs reflect the amount of primary oxidative stress the body is under. Meanwhile, BAP is a measure of the body’s ability to counteract oxidative stress.
- The researchers then calculated the oxidative stress index (OSI), a measure that quantifies the balance between these two factors. OSI was calculated as (d-ROMs/BAP) x 100.
Findings
- The results demonstrated that the d-ROMs levels in the horses were not significantly different between the pre and post-surgery periods. This suggests the surgery did not notably increase the amount of oxidative stress the horses were under.
- However, the BAP of the horses significantly decreased after surgery, showing that their capability to counteract oxidative stress had worsened.
- These results led to a significant increase in OSI post-surgery, reflecting an imbalance between d-ROMs and BAP, indicative of oxidative stress.
Conclusion
- The study’s findings suggest that castration surgery under inhalation anesthesia might induce oxidative stress in Thoroughbred horses.
- The results hint towards a decrease in the horses’ antioxidant potential, leading to an imbalance between the free radicals and antioxidants in their bodies.
- This research provides a fresh perspective in the field of veterinary medicine and could be a baseline for future studies exploring the relationship between surgery and oxidative stress in other animal species or exploring strategies to enhance the bodies’ antioxidant potential before surgery.
Cite This Article
APA
Tsuzuki N, Sasaki N, Kusano K, Endo Y, Torisu S.
(2016).
Oxidative stress markers in Thoroughbred horses after castration surgery under inhalation anesthesia.
J Equine Sci, 27(2), 77-79.
https://doi.org/10.1294/jes.27.77 Publication
Researcher Affiliations
- Laboratory of Veterinary Surgery, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.
- Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan.
- Japan Racing Association Miho Training Center, Ibaraki 300-0493, Japan.
- Japan Racing Association Miyazaki Yearling Training Farm, Miyazaki 880-0036, Japan.
- Veterinary Teaching Hospital, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.
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Citations
This article has been cited 7 times.- Sgorbini M, Bonelli F, Percacini G, Pasquini A, Rota A. Maternal and Neonatal Evaluation of Derived Reactive Oxygen Metabolites and Biological Antioxidant Potential in Donkey Mares and Foals. Animals (Basel) 2021 Oct 3;11(10).
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- Chongphaibulpatana P, Kumagai Y, Fukui D, Katayama M, Uzuka Y. The effect of inspired oxygen concentration on oxidative stress biomarkers in dogs under inhalation anesthesia. Can J Vet Res 2020 Apr;84(2):91-95.
- Kambayashi Y, Tsuzuki N, Tokushige H, Kusano K. Comparison of oxidative stress under different propofol administration protocols in Thoroughbred racehorses by bOS and bAP assessment. J Equine Sci 2018 Sep;29(3):75-78.
- Brkljača Bottegaro N, Gotić J, Šuran J, Brozić D, Klobučar K, Bojanić K, Vrbanac Z. Effect of prolonged submaximal exercise on serum oxidative stress biomarkers (d-ROMs, MDA, BAP) and oxidative stress index in endurance horses. BMC Vet Res 2018 Jul 6;14(1):216.
- Del Prete C, Vastolo A, Pasolini MP, Cocchia N, Montano C, Cutrignelli MI. Effects of maternal dietary supplementation with antioxidants on clinical status of mares and their foal. BMC Vet Res 2024 Sep 11;20(1):404.
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