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Journal of equine veterinary science2023; 123; 104223; doi: 10.1016/j.jevs.2023.104223

Pain Assessment and Acute Phase Response in Donkeys Submitted to Inguinal Orchiectomy.

Abstract: In most animals, pain can compromise physiological functions and delay healing so, rapid detection of pain through behavior and inflammatory reaction with biomarkers are necessary. This study aimed to evaluate pain, physiological variations and Acute Phase Proteins (APP) in donkeys undergoing orchiectomy technique by inguinal access. For this research, 15 male northeastern donkeys kept in extensive management were selected, with a mean age of 4.5±3.1 years. All animals had the same anesthetic protocol, using dissociative anesthesia and local block with lidocaine, followed by orchiectomy by inguinal access. Due to their predisposition to complications, the inguinal technique is the most indicated to minimize complications and excessive inflammation in donkeys' orchiectomy, the donkeys were evaluated regarding behavioral assessment of pain, hematological parameters, APP and the surgical wound, during 0 hour, 24 hours, 48 hours and 72 hours. As for the physiological parameters and APP, no significant differences were observed between times, due to the use of nonsteroidal antiinflammatory drugs. In the macroscopic evaluation of the surgical wound, it was observed that there were no significant differences between the times, with animals presenting mean scores of 1.8±0.414, in 48 hours 1.6 ± 0.507, and in 72 hours 1.6 ± 0.507. Most animals had mild to moderate edema in the scrotum and foreskin regions. As for pain assessment, the average scores were between 2 and 3, representing mild and moderate pain, not requiring intervention. However, further research is needed to elucidate the behavior of PFAs in the face of variables and the creation of new pain scales for animals raised in an extensive system.
Publication Date: 2023-01-08 PubMed ID: 36632948DOI: 10.1016/j.jevs.2023.104223Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

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This study investigates the effects of pain management in donkeys undergoing an orchiectomy procedure through an inguinal approach. By assessing behavioral cues, physiological changes, and acute phase proteins, the researchers aim to improve techniques and care around these common surgical procedures.

Research Design and Methodology:

The research involved a sample of 15 male donkeys, maintained under extensive management conditions. The selected donkeys have a mean age of approximately 4.5 years. As part of the study, all selected donkeys underwent an orchiectomy procedure through an inguinal route. Prior to the surgery, anesthesia was applied to the donkeys including dissociative anesthesia and a local block with lidocaine.

  • Various factors such as behavior indicative of pain, hematological characteristics, Acute Phase Proteins (APP) levels, and the condition of the surgical wound were consistently evaluated at 0, 24, 48, and 72 hours postoperatively.
  • Besides the study focused on finding variations in physiological parameters and APP measurements at different times. The use of nonsteroidal antiinflammatory drugs helped to control significant fluctuations in these parameters.
  • The macroscopic evaluation was performed to identify any significant changes in the surgical wounds across different time intervals.
  • The severity of pain was gauged using a scale that categorized pain levels between 2 and 3, indicating mild to moderate pain and determining if any intervention was required.

Key Findings:

The study concluded with several key observations which could affect future treatment protocols,

  • The research found that most animals experienced only mild or moderate edema in the scrotum and foreskin regions after the operation. The average inflammation scores of the animals did not show significant variation by time, with mean scores narrowly ranging from 1.6 to 1.8.
  • Regarding pain, the average scores were between the crowd range of 2 and 3, equating to mild and moderate pain. The pain levels detected didn’t require direct intervention, according to the study.

Implications:

Though the research provided valuable insights into post-operative care for donkeys undergoing orchiectomy via an inguinal approach, further investigation is suggested by the researchers.

  • The behavior of PFAs (Plasma Fibrinogen Aggregation) under varying variables is one area the researchers singled out for future studies.
  • The development of new animal pain scales specifically for extensively managed animals is proposed as another avenue for in-depth investigation.

Cite This Article

APA
Medeiros do Nascimento RC, Graboschii ACG, da Fonseca LS, Silva AR, Souto PC, da Fonseca LA, Goulart MOF, Escodro PB. (2023). Pain Assessment and Acute Phase Response in Donkeys Submitted to Inguinal Orchiectomy. J Equine Vet Sci, 123, 104223. https://doi.org/10.1016/j.jevs.2023.104223

Publication

ISSN: 0737-0806
NlmUniqueID: 8216840
Country: United States
Language: English
Volume: 123
Pages: 104223

Researcher Affiliations

Medeiros do Nascimento, Rayane Caroline
  • Veterinary department of the Federal University of Alagoas, Viçosa- Alagoas, Brazil.
Graboschii, Amanda Caroline Gomes
  • Veterinary department of the Federal University of Alagoas, Viçosa- Alagoas, Brazil.
da Fonseca, Lucas Santana
  • Veterinary department of the Federal University of Alagoas, Viçosa- Alagoas, Brazil.
Silva, Aline Rocha
  • Veterinary department of the Federal University of Alagoas, Viçosa- Alagoas, Brazil.
Souto, Pollyanna Cordeiro
  • Veterinary department of the Federal University of Viçosa- Viçosa, Minas Gerais, Brazil.
da Fonseca, Leandro Abreu
  • Veterinary department of the Federal University of Viçosa- Viçosa, Minas Gerais, Brazil.
Goulart, Marilia Oliveira Fonseca
  • Veterinary department of the Federal University of Alagoas, Viçosa- Alagoas, Brazil.
Escodro, Pierre Barnabé
  • Veterinary department of the Federal University of Alagoas, Viçosa- Alagoas, Brazil. Electronic address: pierre.escodro@vicosa.ufal.br.

MeSH Terms

  • Male
  • Animals
  • Orchiectomy / adverse effects
  • Orchiectomy / veterinary
  • Orchiectomy / methods
  • Acute-Phase Reaction / veterinary
  • Pain Measurement
  • Equidae / physiology
  • Surgical Wound / veterinary
  • Pain / veterinary

Citations

This article has been cited 2 times.
  1. Alipour-Khairkhah H, Azizi S, Asri-Rezaei S. Stress, lipid profile and inflammatory responses to flunixin meglumine administration in surgical and non-surgical castration in donkeys. Vet Anim Sci 2025 Mar;27:100423.
    doi: 10.1016/j.vas.2024.100423pubmed: 39835330google scholar: lookup
  2. Helal IE, Al-Abbadi HA, Hashem MA, Abdelrazek HMA, Shekidef MH, Ahmed MF. Analgesic Effect of Dexmedetomidine-Nalbuphine Combination vs. Dexmedetomidine Alone in Donkeys Undergoing Field Castration under Total Intravenous Anesthesia. Animals (Basel) 2024 Aug 23;14(17).
    doi: 10.3390/ani14172452pubmed: 39272237google scholar: lookup