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Home / Equine Research Bank / J Mol Endocrinol / Parathyroid hormone (PTH) secretion, PTH mRNA and calcium-se...
Journal of molecular endocrinology2003; 31(3); 609-620; doi: 10.1677/jme.0.0310609

Parathyroid hormone (PTH) secretion, PTH mRNA and calcium-sensing receptor mRNA expression in equine parathyroid cells, and effects of interleukin (IL)-1, IL-6, and tumor necrosis factor-alpha on equine parathyroid cell function.

Abstract: Parathyroid hormone (PTH) is secreted by the chief cells of the parathyroid gland in response to changes in ionized calcium (Ca(2+)) concentrations. In this study, we measured PTH secretion, and PTH mRNA and calcium-sensing receptor (CaR) mRNA expression by equine parathyroid chief cells in vitro. We also evaluated the effects of interleukin (IL)-1beta, IL-6, and tumor necrosis factor (TNF)-alpha on PTH secretion, and PTH and CaR mRNA expression. The relationship between PTH and Ca(2+) was inversely related. PTH secretion decreased from 100% (day 0) to 13% (day 30). PTH mRNA expression declined from 100% (day 0) to 25% (day 30). CaR mRNA decreased from 100% (day 0) to 16% (day 30). Chief cells exposed to high (2.0 mM) Ca(2+) concentrations had a lower PTH mRNA expression compared with low Ca(2+) concentrations. Ca(2+) concentrations had no effect on CaR mRNA expression. The inhibitory effect of high Ca(2+) concentrations on PTH secretion also declined over time. After day 10, there was no significant difference in PTH secretion between low and high Ca(2+ )concentrations. IL-1beta decreased both PTH secretion (75%) and PTH mRNA expression (73%), and resulted in a significant overexpression of CaR mRNA (up to 142%). The effects of IL-1beta were blocked by an IL-1 receptor antagonist. IL-1beta decreased the Ca(2+) set-point from 1.4 mM to 1.2 mM. IL-6 decreased PTH secretion (74%), but had no effect on PTH and CaR mRNA expression. TNF-alpha had no effect on PTH secretion, and PTH and CaR mRNA expression. In summary, the decreased responsiveness of parathyroid cells to Ca(2+) from 0 to 30 days can be explained, in part, by the reduced CaR expression. IL-1beta and IL-6 but not TNF-alpha affected parathyroid function in vitro and may be important in influencing PTH secretion in the septic horse.
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Publication Date: 2003-12-11 PubMed ID: 14664720DOI: 10.1677/jme.0.0310609Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research examines how the secretion and mRNA expression of Parathyroid hormone (PTH) and calcium-sensing receptors in equine parathyroid cells are influenced by different calcium concentrations and various cytokines (interleukins and tumor necrosis factors).

Parathyroid Hormone and Calcium-Sensing Receptors

  • The study observes the parathyroid hormone (PTH) and calcium-sensing receptor (CaR) mRNA expression and secretion by equine parathyroid chief cells in vitro, i.e., outside the living body in a controlled environment.
  • The relationship between PTH and calcium ions is found to be inversely related; that is, as the calcium concentration increases, the PTH secretion and expression decrease.
  • Notably, the expression of PTH and CaR mRNA shows a decline over time, with PTH mRNA expression reaching 25% and CaR mRNA expression reaching 16% by day 30.
  • The study also notes that cells exposed to higher calcium concentrations expressed lower PTH mRNA compared to lower calcium concentrations. Meanwhile, different concentrations of calcium exhibited no effect on CaR mRNA expression.

Effects of Interleukin and Tumor Necrosis Factors

  • The study additionally examines the impact of various cytokines, including Interleukin-1beta (IL-1β), Interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α), on PTH and CaR mRNA expression and secretion.
  • IL-1β is identified to significantly reduce both PTH secretion and mRNA expression and increase CaR mRNA expression. Meanwhile, the impacts of IL-1β could be blocked using an IL-1 receptor antagonist.
  • IL-6 is found to reduce PTH secretion but has no discernable effect on both PTH and CaR mRNA expression.
  • TNF-α, on the other hand, shows no effect on PTH secretion or PTH and CaR mRNA expression.

Summary and Implications

  • The research concludes that the decreased sensitivity of parathyroid cells to the calcium concentrations over a 30-day observation period can be attributed, in part, to the reduced expression of CaR.
  • Furthermore, IL-1β and IL-6 cytokines, but not TNF-α, impacted parathyroid function, suggesting their potential role in influencing PTH secretion in septic horses. This could have implications in treating and managing horses with sepsis.

Cite This Article

APA
Toribio RE, Kohn CW, Capen CC, Rosol TJ. (2003). Parathyroid hormone (PTH) secretion, PTH mRNA and calcium-sensing receptor mRNA expression in equine parathyroid cells, and effects of interleukin (IL)-1, IL-6, and tumor necrosis factor-alpha on equine parathyroid cell function. J Mol Endocrinol, 31(3), 609-620. https://doi.org/10.1677/jme.0.0310609

Publication

Journal of molecular endocrinology
ISSN: 0952-5041
NlmUniqueID: 8902617
Country: England
Language: English
Volume: 31
Issue: 3
Pages: 609-620

Researcher Affiliations

Toribio, R E
  • Department of Veterinary Biosciences, College of Veterinary Medicine, 1925 Coffey Road, The Ohio State University, Columbus, Ohio 43210, USA. toribio.1@osu.edu
Kohn, C W
    Capen, C C
      Rosol, T J

        MeSH Terms

        • Animals
        • Calcium / metabolism
        • Cells, Cultured
        • Horses / metabolism
        • Interleukin-1 / pharmacology
        • Interleukin-6 / pharmacology
        • Parathyroid Glands / metabolism
        • Parathyroid Hormone / metabolism
        • Receptors, Calcium-Sensing / metabolism
        • Tumor Necrosis Factor-alpha / pharmacology

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