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Applied and environmental microbiology1998; 64(11); 4269-4275; doi: 10.1128/AEM.64.11.4269-4275.1998

Pathogenicity of Vibrio alginolyticus for cultured gilt-head sea bream (Sparus aurata L.).

Abstract: The in vivo and in vitro pathogenic activities of whole cells and extracellular products of Vibrio alginolyticus for cultured gilt-head sea bream were evaluated. The 50% lethal doses ranged from 5.4 x 10(4) to 1.0 x 10(6) CFU/g of body weight. The strains examined had the ability to adhere to skin, gill, and intestinal mucus of sea bream and to cultured cells of a chinook salmon embryo cell line. In addition, the in vitro ability of V. alginolyticus to adhere to mucus and skin cells of sea bream was demonstrated by scanning electron microscopy. The biological activities of extracellular products of V. alginolyticus were hydrolytic activities; the products were able to degrade sea bream mucus. V. alginolyticus was cytotoxic for fish cell lines and lethal for sea bream. Moreover, the extracellular products could degrade sea bream tissues. However, experiments performed with the bath immersion inoculation technique demonstrated that V. alginolyticus should be considered a pathogen for sea bream only when the mucus layer is removed and the skin is damaged.
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Publication Date: 1998-10-31 PubMed ID: 9797276PubMed Central: PMC106638DOI: 10.1128/AEM.64.11.4269-4275.1998Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the damaging effects of Vibrio alginolyticus, a type of bacteria, on gilt-head sea bream, both in isolation (in vitro) and in living organisms (in vivo). The research suggests that the bacteria is harmful to the fish only when their protective mucus layer is removed or their skin is damaged.

Understanding Vibrio alginolyticus Pathogenicity

  • This study aims at understanding the pathogenic abilities of Vibrio alginolyticus, a type of marine bacteria, on gilt-head sea bream, a marine fish commonly cultured for food.
  • Researchers discovered that Vibrio alginolyticus can be lethal to the sea bream, with the lethal doses ranging from 5.4 x 10(4) to 1.0 x 10(6) CFU/g of the fish’s body weight.
  • The specific strains of Vibrio alginolyticus examined demonstrated an ability to bind to the skin, gills, and intestinal mucus of the sea bream, thus exhibiting a strong affinity for the fish.

In Vitro and In Vivo Observations

  • In a controlled environment (in vitro), Vibrio alginolyticus also demonstrated an ability to bind to the mucus and skin cells of cultured sea bream. These observations were further confirmed using scanning electron microscopy.
  • The virulence factor of Vibrio alginolyticus comes from its extracellular products, which demonstrated hydrolytic activity capable of degrading the sea bream’s protective mucus layer.
  • It was also found that Vibrio alginolyticus is cytotoxic, causing cell damage for fish cell lines, and exerted lethal effects on sea bream. The microorganism’s extracellular products could degrade the fish’s tissues as well.

Conditional Pathogenicity

  • Despite these findings, experiments that used the bath immersion inoculation technique suggested that Vibrio alginolyticus should be seen as a pathogen for sea bream only under specific conditions. These conditions include when the mucus layer — an essential part of the fish’s immune defense — is removed and when the skin is damaged.
  • The study’s findings therefore stress the need to maintain a healthy mucus layer and skin in gilt-head sea bream to prevent infections caused by Vibrio alginolyticus.

Cite This Article

APA
Balebona MC, Andreu MJ, Bordas MA, Zorrilla I, Moriñigo MA, Borrego JJ. (1998). Pathogenicity of Vibrio alginolyticus for cultured gilt-head sea bream (Sparus aurata L.). Appl Environ Microbiol, 64(11), 4269-4275. https://doi.org/10.1128/AEM.64.11.4269-4275.1998

Publication

Applied and environmental microbiology
ISSN: 0099-2240
NlmUniqueID: 7605801
Country: United States
Language: English
Volume: 64
Issue: 11
Pages: 4269-4275

Researcher Affiliations

Balebona, M C
  • Departments of Microbiology, Faculty of Sciences, University of Malaga, Campus de Teatinos, 29071 Malaga, Spain.
Andreu, M J
    Bordas, M A
      Zorrilla, I
        Moriñigo, M A
          Borrego, J J

            MeSH Terms

            • Animals
            • Bacterial Adhesion
            • Bacterial Toxins / toxicity
            • Cell-Free System
            • Erythrocytes / drug effects
            • Fish Diseases / microbiology
            • Fish Diseases / pathology
            • Gills / microbiology
            • Hemagglutination Tests
            • Horses
            • Intestinal Mucosa / microbiology
            • Muscle, Skeletal / microbiology
            • Muscle, Skeletal / pathology
            • Perciformes / microbiology
            • Skin / microbiology
            • Skin / pathology
            • Skin / ultrastructure
            • Vibrio / isolation & purification
            • Vibrio / pathogenicity
            • Vibrio / physiology
            • Vibrio Infections / microbiology
            • Vibrio Infections / pathology
            • Vibrio Infections / veterinary
            • Virulence

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            This article has been cited 38 times.
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