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Journal of virology1990; 64(4); 1839-1843; doi: 10.1128/JVI.64.4.1839-1843.1990

Pattern of transcription of the genome of equine infectious anemia virus.

Abstract: The pattern of expression of the equine infectious anemia virus (EIAV) genome in a persistently infected canine cell line was determined. Five EIAV-specific transcripts (8.2, 5.0, 4.0, 2, and 1.8 kilobases [kb]) were detected by using subgenomic restriction enzyme fragments of EIAV DNA and EIAV-specific oligonucleotides as probes. The 8.2-kb mRNA could be shown to represent viral genomic RNA, whereas the smaller transcripts were generated by splicing events. Evidence was obtained that indicated that each subgenomic RNA species shared a common 5'-splice donor. The 5.0-kb mRNA was found to be expressed at relatively low levels, was difficult to detect consistently, and appeared to be generated by a single splicing event which linked the 5' exon to the 3' region of pol. The 4.0-kb transcript was concluded to be the env mRNA on the basis of its hybridization pattern with the various probes and its abundance. The 2-kb species was found to be multiply spliced and was encoded by sequences derived from orf2 but was not detected by probes representing 3'-env/3'-orf sequences. The 1.8-kb species was shown to consist of sequences representing orf1, part of orf2, and the 3'-orf/env and may represent the message for the EIAV trans-activator gene.
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Publication Date: 1990-04-01 PubMed ID: 2157066PubMed Central: PMC249326DOI: 10.1128/JVI.64.4.1839-1843.1990Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the pattern of genome expression of the equine infectious anemia virus (EIAV) in a persistently infected dog cell line, identifying five specific transcripts and examining their features and related gene functions.

Methodology

The researchers performed a detailed study on a persistently infected canine cell line to determine the expression pattern of the EIAV genome. This was done by:

  • Detecting five distinct EIAV-specific transcripts (measured in kilobases [kb])
  • Using subgenomic restriction enzyme fragments of EIAV DNA as well as EIAV-specific oligonucleotides as probes
  • Analysis of the splicing events of these transcripts

Findings

The research findings can be summarized as follows:

  • The 8.2-kb mRNA is the representation of the viral genomic RNA, which was confirmed through the smaller transcripts that were produced by splicing events.
  • Every subgenomic RNA species share a common 5′-splice donor
  • The 5.0-kb mRNA has a relatively low level of expression, is difficult to detect consistently, and appears to be produced through a solitary splicing event which links the 5′ exon to the 3′ region of pol.
  • The 4.0-kb transcript is likely the env mRNA, supported by its hybridization pattern with different probes and its relative abundance.
  • The 2-kb species is multiply spliced and is encoded by sequences that come from orf2, but is not detected by probes that represent 3′-env/3′-orf sequences.
  • The 1.8-kb species contains sequences that represent orf1, some of orf2, and the 3′-orf/env and could perhaps be the message for the EIAV trans-activator gene.

Conclusions

These findings provide critical insights into the genome expression of EIAV in a persistently infected dog cell culture, revealing detailed attributes and potential functions of the identified transcripts. These findings contribute valuable knowledge to the scientific understanding of EIAV and could potentially lead to more effective treatments for this virus in the future.

Cite This Article

APA
Noiman S, Yaniv A, Sherman L, Tronick SR, Gazit A. (1990). Pattern of transcription of the genome of equine infectious anemia virus. J Virol, 64(4), 1839-1843. https://doi.org/10.1128/JVI.64.4.1839-1843.1990

Publication

Journal of virology
ISSN: 0022-538X
NlmUniqueID: 0113724
Country: United States
Language: English
Volume: 64
Issue: 4
Pages: 1839-1843

Researcher Affiliations

Noiman, S
  • Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Israel.
Yaniv, A
    Sherman, L
      Tronick, S R
        Gazit, A

          MeSH Terms

          • Amino Acid Sequence
          • Animals
          • Base Sequence
          • Cell Line
          • Genes, Viral
          • Infectious Anemia Virus, Equine / genetics
          • Molecular Sequence Data
          • Nucleic Acid Hybridization
          • RNA Splicing / genetics
          • RNA, Messenger / biosynthesis
          • RNA, Viral / biosynthesis
          • Transcription, Genetic
          • Viral Proteins / genetics

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          Citations

          This article has been cited 12 times.
          1. Li J, Zhang X, Bai B, Zhang M, Ma W, Lin Y, Wang X, Wang XF. Identification of a Novel Post-transcriptional Transactivator from the Equine Infectious Anemia Virus. J Virol 2022 Dec 21;96(24):e0121022.
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