PCR detection of bovine papilloma virus DNA in superficial swabs and scrapings from equine sarcoids.
Abstract: The purpose of this study was to examine if bovine papilloma virus (BPV) DNA can be detected in superficial swabs or scrapings from equine sarcoids. Samples were obtained from 92 sarcoids and 20 non-sarcoidal control lesions. The polymerase chain reaction technique was used with a first primer set to check whether DNA extraction was successful, and with a second primer set specific for BPV-DNA. DNA isolation was successful in 88% of the swabs and 93% of the scrapings. All control lesions were negative for BPV-DNA.
Publication Date: 2001-05-16 PubMed ID: 11352485DOI: 10.1053/tvjl.2000.0524Google Scholar: Lookup
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Summary
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This study explored whether DNA from bovine papilloma virus (BPV) can be detected in superficial swabs and scrapings taken from horse sarcoids, with the polymerase chain reaction technique confirming successful detection.
Research Purpose
The primary goal of this research was to determine if the DNA of bovine papilloma virus (BPV) can be identified in superficial swabs or scrapings taken from equine sarcoids. This investigation aimed at providing insights into the involvement of BPV in equine sarcoids pathogenesis.
- Equine sarcoids, skin tumours in horses, are suspected to be linked with BPV.
- Understanding the presence and role of BPV in these sarcoids could help in developing more effective treatment strategies for affected horses.
Methods
The research was conducted on samples obtained from equine sarcoids and non-sarcoidal control lesions.
- The researchers collected a total of 92 sarcoidal samples and 20 non-sarcoidal control lesions.
- These samples were then subjected to polymerase chain reaction (PCR) technique – an established method used in molecular biology to make numerous copies of a specific DNA segment.
- The PCR technique was employed with a first primer set to ascertain whether DNA extraction was successful and a second specific set for detecting BPV-DNA.
Findings
The results of the study demonstrated a high success rate in DNA extraction and a clear presentation of BPV in the sarcoid samples.
- The researchers were able to successfully isolate DNA in 88% of the swabs and 93% of the scrapings.
- All the non-sarcoidal control lesions were found to be negative for BPV-DNA, indicating the specificity of the BPV presence in sarcoidal samples.
- The high rate of successful extraction suggested that the PCR technique was broadly reliable and effective.
Significance of the Research
This research holds significant implications for understanding the pathogenesis of equine sarcoids and the potential role that BPV plays in this.
- The clear presence of BPV-DNA in equine sarcoid samples, and not in the control samples, strongly suggested an association between the virus and the skin tumours.
- Understanding this link could open up potential avenues for new treatment methods for equine sarcoids.
Cite This Article
APA
Martens A, De Moor A, Ducatelle R.
(2001).
PCR detection of bovine papilloma virus DNA in superficial swabs and scrapings from equine sarcoids.
Vet J, 161(3), 280-286.
https://doi.org/10.1053/tvjl.2000.0524 Publication
Researcher Affiliations
- Department of Large Animal Surgery and Anaesthesiology, Ghent University, Salisburylaan, Belgium. ann.martens@rug.ac.be
MeSH Terms
- Animals
- Bovine papillomavirus 1 / genetics
- Bovine papillomavirus 1 / isolation & purification
- Cattle
- DNA Primers
- DNA, Viral / isolation & purification
- Horse Diseases / diagnosis
- Horse Diseases / pathology
- Horse Diseases / virology
- Horses
- Papillomavirus Infections / diagnosis
- Papillomavirus Infections / veterinary
- Polymerase Chain Reaction / veterinary
- Predictive Value of Tests
- Sarcoidosis / diagnosis
- Sarcoidosis / veterinary
- Sarcoidosis / virology
- Sensitivity and Specificity
- Skin Neoplasms / diagnosis
- Skin Neoplasms / veterinary
- Skin Neoplasms / virology
- Tumor Virus Infections / diagnosis
- Tumor Virus Infections / veterinary
Citations
This article has been cited 16 times.- Jindra C, Hainisch EK, Brandt S. Immunotherapy of Equine Sarcoids-From Early Approaches to Innovative Vaccines.. Vaccines (Basel) 2023 Mar 30;11(4).
- Hamza E, Cosandey J, Gerber V, Koch C, Unger L. The potential of three whole blood microRNAs to predict outcome and monitor treatment response in sarcoid-bearing equids.. Vet Res Commun 2023 Jan;47(1):87-98.
- Gysens L, Vanmechelen B, Haspeslagh M, Maes P, Martens A. New approach for genomic characterisation of equine sarcoid-derived BPV-1/-2 using nanopore-based sequencing.. Virol J 2022 Jan 6;19(1):8.
- Cosandey J, Hamza E, Gerber V, Ramseyer A, Leeb T, Jagannathan V, Blaszczyk K, Unger L. Diagnostic and prognostic potential of eight whole blood microRNAs for equine sarcoid disease.. PLoS One 2021;16(12):e0261076.
- Jindra C, Hainisch EK, Rümmele A, Wolschek M, Muster T, Brandt S. Influenza virus vector iNS1 expressing bovine papillomavirus 1 (BPV1) antigens efficiently induces tumour regression in equine sarcoid patients.. PLoS One 2021;16(11):e0260155.
- Unger L, Abril C, Gerber V, Jagannathan V, Koch C, Hamza E. Diagnostic potential of three serum microRNAs as biomarkers for equine sarcoid disease in horses and donkeys.. J Vet Intern Med 2021 Jan;35(1):610-619.
- Weber LA, Funtan A, Paschke R, Delarocque J, Kalbitz J, Meißner J, Feige K, Kietzmann M, Cavalleri JV. In vitro assessment of triterpenoids NVX-207 and betulinyl-bis-sulfamate as a topical treatment for equine skin cancer.. PLoS One 2020;15(11):e0241448.
- Martano M, Altamura G, Power K, Restucci B, Carella F, Borzacchiello G, Maiolino P. Evaluation of Hypoxia-Inducible Factor-1 Alpha (HIF-1α) in Equine Sarcoid: An Immunohistochemical and Biochemical Study.. Pathogens 2020 Jan 14;9(1).
- Haspeslagh M, Jordana Garcia M, Vlaminck LEM, Martens AM. Topical use of 5% acyclovir cream for the treatment of occult and verrucous equine sarcoids: a double-blinded placebo-controlled study.. BMC Vet Res 2017 Oct 6;13(1):296.
- Araldi RP, Assaf SMR, Carvalho RF, Carvalho MACR, Souza JM, Magnelli RF, Módolo DG, Roperto FP, Stocco RC, Beçak W. Papillomaviruses: a systematic review.. Genet Mol Biol 2017 Jan-Mar;40(1):1-21.
- Martano M, Corteggio A, Restucci B, De Biase ME, Borzacchiello G, Maiolino P. Extracellular matrix remodeling in equine sarcoid: an immunohistochemical and molecular study.. BMC Vet Res 2016 Feb 2;12:24.
- Mosseri S, Hetzel U, Hahn S, Michaloupoulou E, Sallabank HC, Knottenbelt DC, Kipar A. Equine sarcoid: In situ demonstration of matrix metalloproteinase expression.. Vet J 2014 Nov;202(2):279-85.
- Lunardi M, de Alcântara BK, Otonel RA, Rodrigues WB, Alfieri AF, Alfieri AA. Bovine papillomavirus type 13 DNA in equine sarcoids.. J Clin Microbiol 2013 Jul;51(7):2167-71.
- Woolford L, Bennett MD, Sims C, Thomas N, Friend JA, Nicholls PK, Warren KS, O'Hara AJ. Prevalence, emergence, and factors associated with a viral papillomatosis and carcinomatosis syndrome in wild, reintroduced, and captive western barred bandicoots (Perameles bougainville).. Ecohealth 2009 Sep;6(3):414-25.
- Li L, Barry P, Yeh E, Glaser C, Schnurr D, Delwart E. Identification of a novel human gammapapillomavirus species.. J Gen Virol 2009 Oct;90(Pt 10):2413-2417.
- Woolford L, Rector A, Van Ranst M, Ducki A, Bennett MD, Nicholls PK, Warren KS, Swan RA, Wilcox GE, O'Hara AJ. A novel virus detected in papillomas and carcinomas of the endangered western barred bandicoot (Perameles bougainville) exhibits genomic features of both the Papillomaviridae and Polyomaviridae.. J Virol 2007 Dec;81(24):13280-90.
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