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Equine veterinary journal1976; 8(4); 150-155; doi: 10.1111/j.2042-3306.1976.tb03327.x

Percutaneous needle muscle biopsy in the horse.

Abstract: The use of the technique of percutaneous needle biopsy in obtaining skeletal muscle samples in the horse is described. The biochemical, ultrastructural and histochemical investigations that can be carried out on this biopsy specimen are outlined. Analyses performed on the specimen may be used to obtain information on racing potential and state of fitness. These studies on normal horses will provide information for future investigations into the structural and biochemical alterations in muscle disorders in the equine.
Publication Date: 1976-10-01 PubMed ID: 976229DOI: 10.1111/j.2042-3306.1976.tb03327.xGoogle Scholar: Lookup
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  • Journal Article

Summary

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This research discusses the application of percutaneous needle biopsy in obtaining skeletal muscle samples in horses and how these may be used to evaluate racing performance and fitness levels.

Methodology

  • The technique being discussed in this paper is percutaneous needle biopsy, a medical procedure used to extract muscle samples from horses. It’s a minimally invasive process that causes less risk and trauma to the subject as compared to surgical biopsy.
  • The primary focus is to obtain skeletal muscle samples, which hold a wealth of information on the physical state and capabilities of the horse.

Investigations Conducted

  • The extracted samples are subjected to biochemical, ultrastructural, and histochemical investigations. These studies aim to explore the sample at a molecular level, a cellular level and also investigate the chemical compositions present in the tissue.
  • These analyses can provide a broad range of information about the horse’s physiology. This might include elements like metabolic rates, muscle fibre composition, and inherent genetic strengths or weaknesses.

Application and Future Investigations

  • The information derived from such analyses can provide insights into a horse’s racing potential as well as its current fitness status. Trainers and horse owners can use this information to gauge a horse’s capabilities and predict the outcomes of races.
  • The researchers envision an application of these findings to future investigations into muscle disorders in horses. Understanding what constitutes a ‘normal’ muscle composition can inform veterinarians and researchers about the structural and biochemical deviations that might indicate a disease or disorder.

To conclude, this research explains the method and implications of using percutaneous needle biopsy to extract skeletal muscle samples in horses. It paves the way for effective performance assessment and for future studies into equine muscle disorders.

Cite This Article

APA
Snow DH, Guy PS. (1976). Percutaneous needle muscle biopsy in the horse. Equine Vet J, 8(4), 150-155. https://doi.org/10.1111/j.2042-3306.1976.tb03327.x

Publication

ISSN: 0425-1644
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 8
Issue: 4
Pages: 150-155

Researcher Affiliations

Snow, D H
    Guy, P S

      MeSH Terms

      • Animals
      • Biopsy, Needle / veterinary
      • Glycogen / metabolism
      • Histocytochemistry
      • Horses / anatomy & histology
      • Horses / metabolism
      • Muscles / metabolism
      • Muscles / ultrastructure
      • Myofibrils / ultrastructure
      • Sarcoplasmic Reticulum / ultrastructure

      Citations

      This article has been cited 6 times.
      1. Donnelly CG, Burns E, Easton-Jones CA, Katzman S, Stuart R, Cook SE, Finno CJ. Safety and efficacy of subcutaneous alpha-tocopherol in healthy adult horses. Equine Vet Educ 2021 Apr;33(4):215-219.
        doi: 10.1111/eve.13308pubmed: 34326575google scholar: lookup
      2. Williams ZJ, Velez-Irizarry D, Gardner K, Valberg SJ. Integrated proteomic and transcriptomic profiling identifies aberrant gene and protein expression in the sarcomere, mitochondrial complex I, and the extracellular matrix in Warmblood horses with myofibrillar myopathy. BMC Genomics 2021 Jun 11;22(1):438.
        doi: 10.1186/s12864-021-07758-0pubmed: 34112090google scholar: lookup
      3. Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D. Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 2012;7(4):e34890.
        doi: 10.1371/journal.pone.0034890pubmed: 22529950google scholar: lookup
      4. Harris RC, Foster CV. Changes in muscle free carnitine and acetylcarnitine with increasing work intensity in the Thoroughbred horse. Eur J Appl Physiol Occup Physiol 1990;60(2):81-5.
        doi: 10.1007/BF00846025pubmed: 2335175google scholar: lookup
      5. Harris RC, Marlin DJ, Snow DH, Harkness RA. Muscle ATP loss and lactate accumulation at different work intensities in the exercising Thoroughbred horse. Eur J Appl Physiol Occup Physiol 1991;62(4):235-44.
        doi: 10.1007/BF00571546pubmed: 2044532google scholar: lookup
      6. Guy PS, Snow DH. The effect of training and detraining on muscle composition in the horse. J Physiol 1977 Jul;269(1):33-51.
        doi: 10.1113/jphysiol.1977.sp011891pubmed: 142828google scholar: lookup