Performance evaluation of the Sysmex pocH-100iV Diff hematology analyzer for analysis of canine, feline, equine, and bovine blood.
Abstract: The Sysmex pocH-100iV Diff is an impedance hematology analyzer recently introduced for point-of-care use in veterinary practices in Europe. Objective: The purpose of this study was to validate the pocH-100iV Diff for analysis of blood samples from dogs, cats, horses, and cattle. Methods: Fresh EDTA-blood samples from healthy and ill dogs (115), cats (94), horses (91), and cattle (78) were analyzed on the pocH-100iV Diff and the Cell-Dyn 3500. Results of the automated WBC differential counts were compared with the manual differential counts for 77 dogs, 65 cats, 40 horses, and 46 cattle. HCT were compared with PCVs obtained by microhematocrit centrifugation. Furthermore, precision, linearity, carry-over, cell aging, and clinical relevance of the pocH-100iV Diff results were assessed. Results: Most of the CBC results obtained by the pocH-100iV Diff correlated well with those of the Cell-Dyn 3500. Slightly low correlation was observed for canine MCV and hemoglobin concentration. Lymphocytes correlated well in horses and cattle, but less well in cats and dogs. The mixed cell population termed "OTHRS" (all granulocytes and monocytes for horses and cattle; neutrophils, monocytes, and basophils for cats and dogs) correlated well in all tested species. The instrument overestimated feline and canine eosinophils. In cats, platelet counts showed a strong negative bias. Conclusions: The overall performance of the pocH-100iV Diff was excellent with the noted limitations. The automated differential count can be used as screening tool in conjunction with evaluation of a blood smear.
© 2011 American Society for Veterinary Clinical Pathology.
Publication Date: 2011-11-03 PubMed ID: 22092369DOI: 10.1111/j.1939-165X.2011.00372.xGoogle Scholar: Lookup
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- Evaluation Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article validates the effectiveness of the Sysmex pocH-100iV Diff, a hematology analyzer, for analyzing blood samples from various animals. The study includes a performance comparison with another testing tool and highlights some limitations of the analyzer.
Research Objective
- The central aim of the study was to evaluate the efficacy of the Sysmex pocH-100iV Diff analyzer for blood samples taken from dogs, cats, horses, and cattle. The performance of the Sysmex pocH-100iV Diff analyzer was compared with that of the Cell-Dyn 3500 to corroborate results.
Methods
- EDTA-blood samples from both healthy and sick dogs, cats, horses, and cattle were used, ensuring a varied sample population.
- The researchers also compared the results of automated WBC differential counts from the Sysmex pocH-100iV Diff with manual differential counts from a selection of the sampled animals.
- The consistency, linearity, carry-over, cell aging, and clinical implications of results from Sysmex pocH-100iV Diff were also evaluated in this study.
Results
- The results obtained from most of the Complete Blood Count (CBC) tests conducted using the Sysmex pocH-100iV Diff analyzer align well with the test results of the Cell-Dyn 3500.
- However, some limitations were observed, such as a slightly lower correlation in canine Mean Corpuscular Volume (MCV) and hemoglobin concentration.
- Lymphocytes saw a strong correlation in horses and cattle but a weaker one in cats and dogs.
- The instrument tends to overestimate the eosinophil count in dogs and cats.
- In cats, a significantly negative bias was seen in the platelet counts.
Conclusion
- The overall performance of the Sysmex pocH-100iV Diff analyzer was determined to be excellent despite a few limitations.
- The automated differential count generated by the analyzer can be used as an effective screening tool, when used alongside a blood smear evaluation.
Cite This Article
APA
Riond B, Weissenbacher S, Hofmann-Lehmann R, Lutz H.
(2011).
Performance evaluation of the Sysmex pocH-100iV Diff hematology analyzer for analysis of canine, feline, equine, and bovine blood.
Vet Clin Pathol, 40(4), 484-495.
https://doi.org/10.1111/j.1939-165X.2011.00372.x Publication
Researcher Affiliations
- Clinical Laboratory, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
- Clinical Laboratory, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
- Clinical Laboratory, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
- Clinical Laboratory, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
MeSH Terms
- Animals
- Blood Cell Count / instrumentation
- Blood Cell Count / veterinary
- Cats / blood
- Cattle / blood
- Cellular Senescence
- Dogs / blood
- Electric Impedance
- Horses / blood
- Point-of-Care Systems
- Reference Values
- Reproducibility of Results
- Sensitivity and Specificity
Citations
This article has been cited 7 times.- Iba T, Sawada T, Kondo Y, Kondo K, Levy JH. Morphological Changes in Blood Cells in a Rat Model of Heatstroke: A Pilot Study.. J Clin Med 2022 Aug 17;11(16).
- Vedovatto M, da Silva Pereira C, Cortada Neto IM, Moriel P, Morais MDG, Franco GL. Effect of a trace mineral injection at weaning on growth, antioxidant enzymes activity, and immune system in Nellore calves.. Trop Anim Health Prod 2020 Mar;52(2):881-886.
- Joerling J, Doll K. Monitoring of iron deficiency in calves by determination of serum ferritin in comparison with serum iron: A preliminary study.. Open Vet J 2019 Jul;9(2):177-184.
- Akagami M, Oya S, Kashima Y, Seki S, Ouchi Y, Hayama Y. A hematologic key for bovine leukemia virus screening in Japanese black cattle.. J Vet Diagn Invest 2019 Jul;31(4):568-571.
- Nentwig A, Meli ML, Schrack J, Reichler IM, Riond B, Gloor C, Howard J, Hofmann-Lehmann R, Willi B. First report of Cytauxzoon sp. infection in domestic cats in Switzerland: natural and transfusion-transmitted infections.. Parasit Vectors 2018 May 10;11(1):292.
- Nesina S, Katrin Helfer-Hungerbuehler A, Riond B, Boretti FS, Willi B, Meli ML, Grest P, Hofmann-Lehmann R. Retroviral DNA--the silent winner: blood transfusion containing latent feline leukemia provirus causes infection and disease in naïve recipient cats.. Retrovirology 2015 Dec 21;12:105.
- Fujino Y, Nakamura Y, Matsumoto H, Fukushima K, Takahashi M, Ohno K, Tsujimoto H. Development and evaluation of a novel in-clinic automated hematology analyzer, ProCyte Dx, for canine erythrocyte indices, leukogram, platelet counts and reticulocyte counts.. J Vet Med Sci 2013 Nov;75(11):1519-24.
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