Pericytes and their potential in regenerative medicine across species.
Abstract: The discovery that pericytes are in vivo counterparts of Mesenchymal Stem/Stromal Cells (MSCs) has placed these perivascular cells in the research spotlight, bringing up hope for a well-characterized cell source for clinical applications, alternative to poorly defined, heterogeneous MSCs preparations currently in use. Native pericytes express typical MSC markers and, after isolation by fluorescence-activated cell sorting, display an MSC phenotype in culture. These features have been demonstrated in different species, including humans and horses, the main targets of regenerative treatments. Significant clinical potential of pericytes has been shown by transplantation of human cells into rodent models of tissue injury, and it is hoped that future studies will demonstrate clinical potential in veterinary species. Here, we provide an overview of the current knowledge on pericytes across different species including humans, companion and large animal models, in relation to their identification in different body tissues, methodology for prospective isolation, characterization, and potential for tissue regeneration. © 2017 International Society for Advancement of Cytometry.
© 2017 International Society for Advancement of Cytometry.
Publication Date: 2017-09-20 PubMed ID: 28941046DOI: 10.1002/cyto.a.23243Google Scholar: Lookup
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Summary
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This research study is centered around the potential clinical application of pericytes, which are cells found in the body. The study particularly highlights their similarities with Mesenchymal Stem/Stromal Cells (MSCs), and their potential to aid in tissue regeneration, which could have beneficial uses in regenerative medicine.
Understanding Pericytes and Mesenchymal Stem/Stromal Cells
- Pericytes are a type of cell found lining the blood vessels within the body.
- These have been discovered to be in vivo counterparts (the equivalents without any laboratory modification) of Mesenchymal Stem/Stromal Cells (MSCs), which are multipotent stem cells with the ability to differentiate into a variety of cell types including osteoblasts (bone cells), chondrocytes (cartilage cells), etc.
- The resemblance to MSCs makes pericytes a prominent research material, with potential use in clinical applications as opposed to MSCs. This is due to MSC preparations being poorly defined and heterogeneous.
- The study notes these features of pericytes have been demonstrated across species, inclusive of humans and horses, which are the primary targets of regenerative treatments.
Prospective Isolation and Characterization of Pericytes
- Furthermore, the research details the methodology of isolating pericytes using fluorescence-activated cell sorting, a specialized type of flow cytometry, and how they display an MSC phenotype when cultured.
- The identification of pericytes in different body tissues, along with their prospective isolation and characterization, are essential to understand their potential role in tissue regeneration.
Potential Clinical Applications
- The paper shows significant clinical potential of pericytes by transplanting human cells into rodent models of tissue injury. The resultant evidenced capacity for tissue regeneration offers hope for their future use in veterinary species, and potentially within the medical field.
- While the conclusions are drawn from model organisms, the researchers are optimistic that future studies will validate their findings across other species, broadening the application of this research.
Cite This Article
APA
Esteves CL, Donadeu FX.
(2017).
Pericytes and their potential in regenerative medicine across species.
Cytometry A, 93(1), 50-59.
https://doi.org/10.1002/cyto.a.23243 Publication
Researcher Affiliations
- Division of Developmental Biology, The Roslin Institute, University of Edinburgh, Edinburgh EH25 9RG, United Kingdom.
- Division of Developmental Biology, The Roslin Institute, University of Edinburgh, Edinburgh EH25 9RG, United Kingdom.
MeSH Terms
- Animals
- Antigens, CD / metabolism
- Cell Separation / methods
- Dogs
- Horses
- Humans
- Pericytes / cytology
- Pericytes / physiology
- Pericytes / transplantation
- Regenerative Medicine / methods
- Regenerative Medicine / trends
- Sheep
- Species Specificity
- Swine
Citations
This article has been cited 13 times.- Phyo H, Aburza A, Mellanby K, Esteves CL. Characterization of canine adipose- and endometrium-derived Mesenchymal Stem/Stromal Cells and response to lipopolysaccharide.. Front Vet Sci 2023;10:1180760.
- Marr N, Zamboulis DE, Werling D, Felder AA, Dudhia J, Pitsillides AA, Thorpe CT. The tendon interfascicular basement membrane provides a vascular niche for CD146+ cell subpopulations.. Front Cell Dev Biol 2022;10:1094124.
- Rios-Arce ND, Murugesh DK, Hum NR, Sebastian A, Jbeily EH, Christiansen BA, Loots GG. Preexisting Type 1 Diabetes Mellitus Blunts the Development of Posttraumatic Osteoarthritis.. JBMR Plus 2022 May;6(5):e10625.
- Surowiecka A, Strużyna J. Adipose-Derived Stem Cells for Facial Rejuvenation.. J Pers Med 2022 Jan 16;12(1).
- Zhuang L, Visalakshan RM, Kaur P. Dermal Pericytes Exhibit Declined Ability to Promote Human Skin Regeneration with Ageing in 3D Organotypic Culture Models.. Cells 2021 Nov 6;10(11).
- Juntunen M, Heinonen S, Huhtala H, Rissanen A, Kaprio J, Kuismanen K, Pietiläinen KH, Miettinen S, Patrikoski M. Evaluation of the effect of donor weight on adipose stromal/stem cell characteristics by using weight-discordant monozygotic twin pairs.. Stem Cell Res Ther 2021 Sep 26;12(1):516.
- King TA. The One Medicine concept: its emergence from history as a systematic approach to re-integrate human and veterinary medicine.. Emerg Top Life Sci 2021 Nov 12;5(5):643-654.
- Sebastian A, McCool JL, Hum NR, Murugesh DK, Wilson SP, Christiansen BA, Loots GG. Single-Cell RNA-Seq Reveals Transcriptomic Heterogeneity and Post-Traumatic Osteoarthritis-Associated Early Molecular Changes in Mouse Articular Chondrocytes.. Cells 2021 Jun 10;10(6).
- Weatherall EL, Avilkina V, Cortes-Araya Y, Dan-Jumbo S, Stenhouse C, Donadeu FX, Esteves CL. Differentiation Potential of Mesenchymal Stem/Stromal Cells Is Altered by Intrauterine Growth Restriction.. Front Vet Sci 2020;7:558905.
- Sebastian A, Hum NR, Martin KA, Gilmore SF, Peran I, Byers SW, Wheeler EK, Coleman MA, Loots GG. Single-Cell Transcriptomic Analysis of Tumor-Derived Fibroblasts and Normal Tissue-Resident Fibroblasts Reveals Fibroblast Heterogeneity in Breast Cancer.. Cancers (Basel) 2020 May 21;12(5).
- Almeida-Porada G, Atala AJ, Porada CD. Therapeutic Mesenchymal Stromal Cells for Immunotherapy and for Gene and Drug Delivery.. Mol Ther Methods Clin Dev 2020 Mar 13;16:204-224.
- Dias IE, Pinto PO, Barros LC, Viegas CA, Dias IR, Carvalho PP. Mesenchymal stem cells therapy in companion animals: useful for immune-mediated diseases?. BMC Vet Res 2019 Oct 22;15(1):358.
- Cortés-Araya Y, Amilon K, Rink BE, Black G, Lisowski Z, Donadeu FX, Esteves CL. Comparison of Antibacterial and Immunological Properties of Mesenchymal Stem/Stromal Cells from Equine Bone Marrow, Endometrium, and Adipose Tissue.. Stem Cells Dev 2018 Nov 1;27(21):1518-1525.
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