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Stem cells translational medicine2014; 3(12); 1514-1525; doi: 10.5966/sctm.2014-0138

Peripheral Blood-Derived Mesenchymal Stromal Cells Promote Angiogenesis via Paracrine Stimulation of Vascular Endothelial Growth Factor Secretion in the Equine Model.

Abstract: Mesenchymal stromal cells (MSCs) have received much attention as a potential treatment of ischemic diseases, including ischemic tissue injury and cardiac failure. The beneficial effects of MSCs are thought to be mediated by their ability to provide proangiogenic factors, creating a favorable microenvironment that results in neovascularization and tissue regeneration. To study this in more detail and to explore the potential of the horse as a valuable translational model, the objectives of the present study were to examine the presence of angiogenic stimulating factors in the conditioned medium (CM) of peripheral blood-derived equine mesenchymal stromal cells (PB-MSCs) and to study their in vitro effect on angiogenesis-related endothelial cell (EC) behavior, including proliferation and vessel formation. Our salient findings were that CM from PB-MSCs contained significant levels of several proangiogenic factors. Furthermore, we found that CM could induce angiogenesis in equine vascular ECs and confirmed that endothelin-1, insulin growth factor binding protein 2, interleukin-8, and platelet-derived growth factor-AA, but not urokinase-type plasminogen activator, were responsible for this enhanced EC network formation by increasing the expression level of vascular endothelial growth factor-A, an important angiogenesis stimulator.
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Publication Date: 2014-10-13 PubMed ID: 25313202PubMed Central: PMC4250216DOI: 10.5966/sctm.2014-0138Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article focuses on how mesenchymal stromal cells (MSCs) from horse (equine) peripheral blood can stimulate angiogenesis – the formation of new blood vessels, by promoting the secretion of a growth factor in affected tissues. This could help in treating ischemic diseases, a condition causing restricted blood flow to tissues.

Objective of the Study

  • The primary objective of the research was to examine the presence and role of angiogenic stimulating factors in the conditioned medium (CM) of peripheral blood-derived equine mesenchymal stromal cells (PB-MSCs).
  • Another goal of the study was to understand the impact of these factors on the behaviors of endothelial cells (cells lining the blood vessels) related to angiogenesis such as proliferation and formation of new vessels.

Major Findings of the Study

  • This research found that the conditioned medium from the PB-MSCs contained significant levels of several proangiogenic factors, which are substances that stimulate the formation of blood vessels.
  • The investigators discovered that conditioned medium could induce angiogenesis in equine vascular endothelial cells. The growth factors responsible for this enhanced blood vessel network were endothelin-1, insulin growth factor binding protein 2, interleukin-8, and platelet-derived growth factor-AA.
  • The research also confirmed that a significant angiogenesis stimulator, vascular endothelial growth factor-A, increased expression levels due to the above growth factors, but not as a result of the presence of urokinase-type plasminogen activator.

Implications of the Research

  • The findings of the research offer valuable insights into how the body can naturally repair and regenerate tissues through blood vessel formation, increasing the potential for new treatments for ischemic diseases and tissue injury.
  • The study also supports the potential of using horse models in translational research – translating scientific findings into practical applications for treating such diseases.

Cite This Article

APA
Bussche L, Van de Walle GR. (2014). Peripheral Blood-Derived Mesenchymal Stromal Cells Promote Angiogenesis via Paracrine Stimulation of Vascular Endothelial Growth Factor Secretion in the Equine Model. Stem Cells Transl Med, 3(12), 1514-1525. https://doi.org/10.5966/sctm.2014-0138

Publication

Stem cells translational medicine
ISSN: 2157-6564
NlmUniqueID: 101578022
Country: England
Language: English
Volume: 3
Issue: 12
Pages: 1514-1525

Researcher Affiliations

Bussche, Leen
  • Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA.
Van de Walle, Gerlinde R
  • Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA grv23@cornell.edu.

MeSH Terms

  • Animals
  • Culture Media, Conditioned / pharmacology
  • Endothelial Cells / cytology
  • Endothelial Cells / metabolism
  • Horses
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism
  • Neovascularization, Physiologic
  • Paracrine Communication
  • Vascular Endothelial Growth Factor A / metabolism

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