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Home / Equine Research Bank / J Gen Virol / Peripheral blood mononuclear cells represent a reservoir of ...
The Journal of general virology2008; 89(Pt 6); 1390-1395; doi: 10.1099/vir.0.83568-0

Peripheral blood mononuclear cells represent a reservoir of bovine papillomavirus DNA in sarcoid-affected equines.

Abstract: Bovine papillomaviruses of types 1 and 2 (BPV-1 and -2) chiefly contribute to equine sarcoid pathogenesis. However, the mode of virus transmission and the presence of latent infections are largely unknown. This study established a PCR protocol allowing detection of <or=10 copies of the BPV-1/-2 genes E5 and L1. Subsequent screening of peripheral blood mononuclear cell (PBMC) DNA derived from horses with and without BPV-1/2-induced skin lesions demonstrated the exclusive presence of E5, but not L1, in PBMCs of BPV-1/2-infected equines. To validate this result, a blind PCR was performed from enciphered PBMC DNA derived from 66 horses, revealing E5 in the PBMCs of three individuals with confirmed sarcoids, whereas the remaining 63 sarcoid-free animals were negative for this gene. L1 could not be detected in any PBMC DNA, suggesting either deletion or interruption of this gene in PBMCs of BPV-1/-2-infected equines. These results support the hypothesis that PBMCs may serve as host cells for BPV-1/-2 DNA and contribute to virus latency.
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Publication Date: 2008-05-14 PubMed ID: 18474554DOI: 10.1099/vir.0.83568-0Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research reveals that white blood cells act as host cells for bovine papillomavirus DNA, contributing to its latent phase in horses affected by sarcoids. It also suggests that the transmission mode of this virus and its persistent infections aren’t well understood.

Research Aim and Method

  • The research aims to understand the transmission mode of bovine papillomaviruses types 1 and 2 (BPV-1 and -2) and the occurrence of latent or dormant infections. These viruses are mainly responsible for the formation of equine sarcoids, benign skin tumours affecting horses.
  • The researchers used a PCR protocol, a technique for making multiple copies of a specific DNA segment, to detect less than or equal to 10 copies of the BPV-1/-2 genes E5 and L1. This high sensitivity test allows for the detection of low levels of viral DNA, which is essential to uncover latent or low-level infections.

Findings

  • The screening was performed on the DNA derived from peripheral blood mononuclear cells (PBMCs) from horses with and without BPV-1/2-induced skin lesions. PBMCs are a type of white blood cell that plays a crucial role in the immune system to fight infections.
  • The results demonstrated the presence of the E5 gene exclusively, not L1, in the PBMCs of horses infected with BPV-1/2. This implies that the E5 gene may contain genetic information necessary for the production of viral proteins, contributing to the replication and survival of the virus in host cells.
  • The absence of the L1 gene in any PBMC DNA suggests that this gene might be deleted or interrupted in BPV-1/-2-infected horses. L1 is an important BPV gene as well, coding for a major capsid protein involved in viral packaging and entry into host cells. Its absence raises questions about how the virus is able to replicate and maintain latency.

Conclusion

  • The study concludes that PBMCs may act as host cells for the BPV-1/-2 DNA, contributing to the latent phase of the virus in horses affected by sarcoids. However, the mechanisms of viral transmission, the role of E5, and the apparent absence of L1 needs further exploration for a more comprehensive understanding of BPV-1/-2 infections.

Cite This Article

APA
Brandt S, Haralambus R, Schoster A, Kirnbauer R, Stanek C. (2008). Peripheral blood mononuclear cells represent a reservoir of bovine papillomavirus DNA in sarcoid-affected equines. J Gen Virol, 89(Pt 6), 1390-1395. https://doi.org/10.1099/vir.0.83568-0

Publication

The Journal of general virology
ISSN: 0022-1317
NlmUniqueID: 0077340
Country: England
Language: English
Volume: 89
Issue: Pt 6
Pages: 1390-1395

Researcher Affiliations

Brandt, Sabine
  • Equine Centre, Veterinary University Vienna, Vienna, Austria.
Haralambus, Rhea
  • Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Berne, Berne, Switzerland.
  • Equine Centre, Veterinary University Vienna, Vienna, Austria.
Schoster, Angelika
  • Ontario Veterinary College, University of Guelph, ON, Canada.
Kirnbauer, Reinhard
  • Department of Dermatology, Division of Immunology, Allergy and Infectious Diseases, Medical University Vienna, Vienna, Austria.
Stanek, Christian
  • Equine Centre, Veterinary University Vienna, Vienna, Austria.

MeSH Terms

  • Animals
  • Bovine papillomavirus 1 / genetics
  • Bovine papillomavirus 1 / isolation & purification
  • Capsid Proteins / analysis
  • Capsid Proteins / genetics
  • Genes, Viral / genetics
  • Horse Diseases / blood
  • Horse Diseases / diagnosis
  • Horses
  • Leukocytes, Mononuclear / virology
  • Oncogene Proteins, Viral / analysis
  • Oncogene Proteins, Viral / genetics
  • Papillomavirus Infections / blood
  • Papillomavirus Infections / complications
  • Papillomavirus Infections / diagnosis
  • Papillomavirus Infections / veterinary
  • Polymerase Chain Reaction
  • Sarcoidosis / complications
  • Sarcoidosis / veterinary
  • Sensitivity and Specificity
  • Virology / methods

Citations

This article has been cited 23 times.
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