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Veterinary medicine international2014; 2014; 385392; doi: 10.1155/2014/385392

Peritoneal reactivity evaluation in horses subjected to experimental small colon enterotomy and treated with subcutaneous heparin.

Abstract: Heparin is routinely administered in postoperative abdominal surgery aiming to prevent adhesions formation; however, there is no consensus indicating its effectiveness. This study evaluated the effect of heparin on peritoneal reactivity after abdominal surgery, through the association between peritoneal fluid features and ultrasonographic and laparoscopic examination. Ten adult horses were used: control group (CG) and treated group (TG). Both groups underwent laparotomy and small colon enterotomy. TG received subcutaneous heparin at 150 IU/kg every 12 hours for 5 days. The animals underwent ultrasonography and peritoneal fluid examination prior to enterotomy (M0) 12 hours (M1), 1 day (M2), 2 days (M3), 4 days (M4), 6 days (M5), 10 days (M6), and 14 days after enterotomy (M7) with laparoscopic examination being performed on the fifth postoperative day. Peritoneal inflammatory response was observed in both groups. The peritoneal fluid of TG animals showed higher echogenicity during heparin therapy. No inflammatory difference was observed between groups through peritoneal fluid features, except for the higher D-dimer concentration in CG. On laparoscopy, slightly diffuse peritoneal reactivity for both groups was observed, being higher for TG. Laparoscopy and ultrasonography association allowed detailed access to the abdominal cavity. Ultrasonography assessed the diffuse peritoneal inflammation, and laparoscopy allowed the detailed analysis of the segments. No gross beneficial reactions resulting from the use of heparin on peritoneal reactivity were observed; however, it was observed by D-dimer evaluation that the TG had less fibrin deposition, which is directly related to a lower rate of abdominal adhesions formation.
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Publication Date: 2014-11-11 PubMed ID: 25436172PubMed Central: PMC4243600DOI: 10.1155/2014/385392Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates the impact of heparin, a medicine generally used post abdominal surgery to prevent adhesion formation, on peritoneal reactivity following abdominal surgery in horses. The research highlights that while there were no significant benefits of heparin on peritoneal reactivity, the treated group had less fibrin deposition, which may lead to a lower rate of abdominal adhesions formation.

Research Methodology

  • Ten adult horses were used in the study, divided into a control group (CG) and a treated group (TG). Both groups had abdominal surgeries, which involved making an incision in their small colons (enterotomy).
  • The TG group was given subcutaneous heparin (150 IU/kg) every 12 hours for five days.
  • Ultrasonography and peritoneal fluid examination were conducted before surgery (M0) and at several intervals after surgery – 12 hours (M1), 1 day (M2), 2 days (M3), 4 days (M4), 6 days (M5), 10 days (M6), and 14 days (M7).
  • On the fifth day after surgery, a laparoscopic examination was performed on all the horses.

Findings

  • Both groups demonstrated peritoneal inflammatory response (a reaction of the stomach lining to injury), regardless of heparin treatment.
  • The peritoneal fluid in the TG animals displayed higher echogenicity (ability to reflect ultrasound waves) during heparin therapy.
  • No significant inflammatory differences were identified between the two groups based on the peritoneal fluid features. However, there was a higher concentration of D-dimer, a protein fragment produced when a blood clot gets dissolved in the body, in the control group.
  • The laparoscopic examination showed slightly diffuse peritoneal reactivity (slightly widespread inflammation) for both groups, but the reactivity was higher in the treated group.
  • The study suggests that combining ultrasonography and laparoscopy can facilitate a comprehensive analysis of the abdominal cavity.
  • Although the study did not find any significant benefits of heparin on peritoneal reactivity, the treated group’s ability to reduce fibrin deposition can potentially lower the rate of abdominal adhesion formation.

Cite This Article

APA
Alonso Jde M, Rodrigues KA, Yamada AL, Watanabe MJ, Alves AL, Rodrigues CA, Hussni CA. (2014). Peritoneal reactivity evaluation in horses subjected to experimental small colon enterotomy and treated with subcutaneous heparin. Vet Med Int, 2014, 385392. https://doi.org/10.1155/2014/385392

Publication

Veterinary medicine international
ISSN: 2090-8113
NlmUniqueID: 101524203
Country: United States
Language: English
Volume: 2014
Pages: 385392
PII: 385392

Researcher Affiliations

Alonso, Juliana de Moura
  • School of Veterinary Medicine and Animal Science, UNESP, University Estadual Paulista, P.O. Box 560, 18618-970 Botucatu, SP, Brazil.
Rodrigues, Karoline Alves
  • School of Veterinary Medicine and Animal Science, UNESP, University Estadual Paulista, P.O. Box 560, 18618-970 Botucatu, SP, Brazil.
Yamada, Ana Lúcia Miluzzi
  • School of Veterinary Medicine and Animal Science, UNESP, University Estadual Paulista, P.O. Box 560, 18618-970 Botucatu, SP, Brazil.
Watanabe, Marcos Jun
  • School of Veterinary Medicine and Animal Science, UNESP, University Estadual Paulista, P.O. Box 560, 18618-970 Botucatu, SP, Brazil.
Alves, Ana Liz Garcia
  • School of Veterinary Medicine and Animal Science, UNESP, University Estadual Paulista, P.O. Box 560, 18618-970 Botucatu, SP, Brazil.
Rodrigues, Celso Antonio
  • School of Veterinary Medicine and Animal Science, UNESP, University Estadual Paulista, P.O. Box 560, 18618-970 Botucatu, SP, Brazil.
Hussni, Carlos Alberto
  • School of Veterinary Medicine and Animal Science, UNESP, University Estadual Paulista, P.O. Box 560, 18618-970 Botucatu, SP, Brazil.

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