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Home / Equine Research Bank / J Vet Pharmacol Ther / Pharmacokinetic Profiles of Metamizole Metabolites after Int...
Journal of veterinary pharmacology and therapeutics2021; 44(6); 927-936; doi: 10.1111/jvp.13003

Pharmacokinetic Profiles of Metamizole Metabolites after Intramuscular and Intravenous Administration in Healthy Arabian Horses.

Abstract: Metamizole sodium (MT) is an analgesic and antipyretic drug molecule used in humans, horses, cattle, swine, and dogs. Metamizole rapidly hydrolyzes and turns into methylamino antipyrine (MAA), an active primary metabolite of MT. The present study aims to determine the pharmacokinetic (PK) profiles of MT metabolites after intravenous (IV) and intramuscular (IM) administration into sex of Arabian horses (Equus ferus caballus) using a cross-over study design. The plasma samples were extracted by solid-phase extraction (SPE) method, and plasma concentrations of MT metabolites were analyzed by high-performance liquid chromatography (HPLC). After administrations of MT, plasma concentrations of methylamino antipyrine (MAA), amino antipyrone (AA), and acetylamino antipyrone (AAA) were determined within range of 15 min-12 h. Plasma concentrations of AA and AAA were lower than the plasma concentrations of major metabolite MAA at each sampling point. The PK parameters were statistically evaluated for MT's metabolites between male and female horses and also between IM and IV administrations of PK parameters such as C , t , t , AUC , AUC , λz, Cl and V (p < .05). The AUC /AUC ratio in female and male horses for MAA was 1.19 and 1.13, respectively. The AUC /AUC ratio for AA was lower than those found for MAA. AUC /AUC ratio was statistically significantly different between male and female horses for AA (p < .05). According to these results, some PK parameters such as Cmax, AUC, and MRT, MAA and AA concentrations have shown statistically significant differences by MT administrations.
© 2021 John Wiley & Sons Ltd.
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Publication Date: 2021-08-25 PubMed ID: 34431528DOI: 10.1111/jvp.13003Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates the breakdown and absorption of the drug Metamizole sodium (MT) in Arabian horses, observed through its metabolites. The work monitors concentrations of these substances in the animals’ blood over time, examining differences in uptake based on sex and method of drug delivery.

Research Methods

  • The main research method utilized in the study involved the administration of Metamizole sodium to Arabian horses and then observing the amount of the primary metabolite, methylamino antipyrine (MAA), and two secondary metabolites, amino antipyrone (AA), and acetylamino antipyrone (AAA) in the horses’ blood plasma over a specified time range.
  • These substances were detected and quantified using a high-performance liquid chromatography (HPLC) technique after extraction from the plasma samples via solid-phase extraction (SPE).
  • The research design was cross-over in nature, meaning that the same horses were used to study the effects of the drug when both intramuscular (IM) and intravenous (IV) administration methods were employed.

Key Findings

  • The researchers discovered that the concentration of the major metabolite MAA was consistently higher than the two secondary metabolites, AA and AAA, at all sampled points in the study. This suggests that MAA is the main substance resulting from the breakdown of Metamizole sodium in the horses’ bodies.
  • The study also established statistical differences in the pharmacokinetic parameters for the metabolites based on administration methods and the sex of the horses involved.
  • Important parameters such as peak concentration (C max), total exposure to the drug (AUC), and mean residence time (MRT) showed significant differences in intramuscular versus intravenous administration. Thus, the way the drug is administered could influence how it is broken down and absorbed in horses’ bodies.
  • The AUC ratio, indicative of total drug exposure, was found to be different for MAA in male and female horses, suggesting that sex may influence the absorption and metabolization of Metamizole sodium in horses.

Concluding Remarks

  • Through the findings of this study, researchers concluded that various factors, such as sex and administration method, can influence the breakdown and absorption of Metamizole sodium in horses.
  • This determination has important implications for the therapeutic use of the drug in equine medicine and contributes to a more comprehensive understanding of drug pharmacokinetics in animals.

Cite This Article

APA
Yılmaz İ, Maraş Z, Uğur Y, Erkan Özgür M, Durmaz M, İbrahim Ulusoy H, Erdoğan S. (2021). Pharmacokinetic Profiles of Metamizole Metabolites after Intramuscular and Intravenous Administration in Healthy Arabian Horses. J Vet Pharmacol Ther, 44(6), 927-936. https://doi.org/10.1111/jvp.13003

Publication

Journal of veterinary pharmacology and therapeutics
ISSN: 1365-2885
NlmUniqueID: 7910920
Country: England
Language: English
Volume: 44
Issue: 6
Pages: 927-936

Researcher Affiliations

Yılmaz, İsmet
  • Department of Pharmacology, Faculty of Pharmacy, Inonu University, Malatya, Turkey.
Maraş, Zeynep
  • Department of Analytical Chemistry, Faculty of Pharmacy, Inonu University, Malatya, Turkey.
Uğur, Yılmaz
  • Department of Pharmacy Services, Health Services Vocational School, Inonu University, Malatya, Turkey.
Erkan Özgür, Mustafa
  • Department of Aquaculture, Malatya Turgut Özal University, Doğanşehir Vahap Küçük Vocational High School, Malatya, Turkey.
Durmaz, Murat
  • Ministry of Food, Agriculture and Animal Husbandry, Ankara, Turkey.
İbrahim Ulusoy, Halil
  • Faculty of Pharmacy, Department of Analytical Chemistry, Cumhuriyet University, Sivas, Turkey.
Erdoğan, Selim
  • Department of Analytical Chemistry, Faculty of Pharmacy, Inonu University, Malatya, Turkey.

MeSH Terms

  • Administration, Intravenous / veterinary
  • Analgesics
  • Animals
  • Antipyrine / pharmacokinetics
  • Area Under Curve
  • Cross-Over Studies
  • Dipyrone / pharmacokinetics
  • Female
  • Horses
  • Male

Grant Funding

  • This study was supported by Inonu University Scientific Research Fund (Project number: 2018/1044) for the financial support.

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