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Pharmacokinetics of boldenone and stanozolol and the results of quantification of anabolic and androgenic steroids in race horses and nonrace horses.
Abstract: Anabolic steroids (ABS) boldenone (BL; 1.1 mg/kg) and stanozolol (ST; 0.55 mg/kg) were administered i.m. to horses and the plasma samples collected up to 64 days. Anabolic steroids and androgenic steroids (ANS) in plasma were quantified using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The limit of detection of all analytes was 25 pg/mL. The median absorption (t1/2 partial differential) and elimination (t1/2e) half-lives for BL were 8.5 h and 123.0 h, respectively, and the area under the plasma concentration-time curve (AUCho) was 274.8 ng.h/mL. The median t1/2e for ST was 82.1 h and the was 700.1 ng.h/mL. Peak mean (X+/-SD) plasma concentrations (Cmax) for BL and ST were 1127.8 and 4118.2 pg/mL, respectively. Quantifiable concentrations of ABS and ANS were found in 61.7% of the 988 plasma samples tested from race tracks. In 17.3% of the plasma samples two or more ABS or ANS were quantifiable. Testosterone (TES) concentrations mean (X+/-SE) in racing and nonracing intact males were 241.3+/-61.3 and 490.4+/-35.1 pg/mL, respectively. TES was not quantified in nonracing geldings and female horses, but was in racing females and geldings. Plasma concentrations of endogenous 19-nortestosterone (nandrolone; NA) from racing and nonracing males were 50.2+/-5.5 and 71.8+/-4.6 pg/mL, respectively.
Publication Date: 2007-03-14 PubMed ID: 17348894DOI: 10.1111/j.1365-2885.2007.00824.xGoogle Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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The research investigates the pharmacokinetics of anabolic steroids, boldenone and stanozolol, in horses, and quantifies anabolic and androgenic steroids in plasma samples taken from race horses and nonrace horses, utilizing liquid chromatography-tandem mass spectrometry.
Pharmacokinetics of Boldenone and Stanozolol
- The study involved the collection of plasma samples from horses that were administered anabolic steroids boldenone and stanozolol intramuscularly.
- The boldenone dose was 1.1 mg/kg and the stanozolol dose was 0.55 mg/kg. The plasma samples were collected over a period of 64 days.
- The median half-life for absorption (t1/2 partial differential) and elimination (t1/2e) for boldenone were observed to be 8.5 hours and 123.0 hours respectively.
- The area under the plasma concentration-time curve (AUC) for boldenone was 274.8 ng.h/mL.
- Stanozolol showed a median elimination half-life of 82.1 hours, and an AUC of 700.1 ng.h/mL.
- Peak mean plasma concentrations (Cmax) for boldenone and stanozolol were 1127.8 and 4118.2 pg/mL respectively.
Quantification of Anabolic and Androgenic Steroids
- Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), quantifiable concentrations of anabolic steroids and androgenic steroids were found present in 61.7% of the 988 plasma samples obtained from race tracks.
- In 17.3% of the plasma samples, two or more anabolic steroids or androgenic steroids were quantifiable.
- The average testosterone concentrations in racing and nonracing intact male horses were 241.3 +/- 61.3 and 490.4 +/- 35.1 pg/mL respectively. However, testosterone was not quantified in female horses and nonracing geldings.
- Plasma concentrations of endogenous 19-nortestosterone (nandrolone) in racing and nonracing males were 50.2 +/- 5.5 and 71.8 +/- 4.6 pg/mL respectively.
Cite This Article
APA
Soma LR, Uboh CE, Guan F, McDonnell S, Pack J.
(2007).
Pharmacokinetics of boldenone and stanozolol and the results of quantification of anabolic and androgenic steroids in race horses and nonrace horses.
J Vet Pharmacol Ther, 30(2), 101-108.
https://doi.org/10.1111/j.1365-2885.2007.00824.x Publication
Researcher Affiliations
- School of Veterinary Medicine, New Bolton Center Campus, University of Pennsylvania, Kennett Square, PA 19348, USA. soma@vet.upenn.edu
MeSH Terms
- Anabolic Agents / administration & dosage
- Anabolic Agents / blood
- Anabolic Agents / pharmacokinetics
- Androgens / administration & dosage
- Androgens / blood
- Androgens / pharmacokinetics
- Animals
- Area Under Curve
- Doping in Sports
- Female
- Horses / metabolism
- Injections, Intramuscular / veterinary
- Male
- Physical Conditioning, Animal
- Reproducibility of Results
- Stanozolol / administration & dosage
- Stanozolol / blood
- Stanozolol / pharmacokinetics
- Testosterone / administration & dosage
- Testosterone / analogs & derivatives
- Testosterone / blood
- Testosterone / pharmacokinetics
Citations
This article has been cited 5 times.- Behairy A, El-Sharkawy NI, Saber TM, Soliman MM, Metwally MMM, Abd El-Rahman GI, Abd-Elhakim YM, El Deib MM. The Modulatory Role of Vitamin C in Boldenone Undecylenate Induced Testicular Oxidative Damage and Androgen Receptor Dysregulation in Adult Male Rats. Antioxidants (Basel) 2020 Oct 28;9(11).
- Iwona MŻ, Barbara W, Alicja K, Sebastian W, Katarzyna S, Andrzej P. Control of Anabolic Hormone Residues in Tissues of Slaughter Animals in Poland During the Period of 2011-2015. J Vet Res 2017 Mar;61(1):69-79.
- Dornelles GL, Bueno A, de Oliveira JS, da Silva AS, França RT, da Silva CB, Machado MS, Petry LD, Abdalla FH, Lhamas CL, de Andrade CM. Biochemical and oxidative stress markers in the liver and kidneys of rats submitted to different protocols of anabolic steroids. Mol Cell Biochem 2017 Jan;425(1-2):181-189.
- Krasowski MD, Drees D, Morris CS, Maakestad J, Blau JL, Ekins S. Cross-reactivity of steroid hormone immunoassays: clinical significance and two-dimensional molecular similarity prediction. BMC Clin Pathol 2014;14:33.
- Kollerov VV, Timakova TA, Shutov AA, Donova MV. Boldenone and Testosterone Production from Phytosterol via One-Pot Cascade Biotransformations. J Fungi (Basel) 2024 Nov 28;10(12).
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