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Home / Equine Research Bank / J Clin Microbiol / Phenotypic and genotypic characterization of Arcanobacterium...
Journal of clinical microbiology2008; 47(1); 124-128; doi: 10.1128/JCM.01933-08

Phenotypic and genotypic characterization of Arcanobacterium haemolyticum isolates from infections of horses.

Abstract: The present study was designed to characterize phenotypically and genotypically seven Arcanobacterium haemolyticum strains obtained from infections of six horses. All seven strains showed the cultural and biochemical properties typical of A. haemolyticum and were susceptible to most of the antibiotics tested. The species identification could be confirmed by amplification and sequencing of the 16S rRNA gene and the 16S-23S rRNA intergenic spacer region and by PCR amplification of species-specific parts of the gene encoding phospholipase D in A. haemolyticum. Use of the latter could possibly improve future identification of this generally human pathogenic bacterial species which, according to the present results, seems to occur also in infections of horses.
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Publication Date: 2008-11-19 PubMed ID: 19020059PubMed Central: PMC2620868DOI: 10.1128/JCM.01933-08Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research explores the properties and identification of seven Arcanobacterium haemolyticum strains found in horse infections, confirming their identification through multiple methods and suggesting the possibility of their occurrence in horse as well.

Objective of the Study

  • The primary goal of this research was to study the phenotypic and genotypic characteristics of seven different strains of Arcanobacterium haemolyticum sourced from infections in six horses.

Phenotypic and Genotypic Characteristics

  • Phenotypically, all seven strains displayed cultural and biochemical properties typical of the A. haemolyticum species. This means their physical and bio-chemical characteristics, such as growth behaviour under different conditions and reaction to certain chemicals, were the ones generally expected from this type of bacteria.
  • On a genotypic level, the strains were identified by analysing their genes – specifically the 16S rRNA gene, the 16S-23S rRNA intergenic spacer region and parts of the gene encoding phospholipase D in A. haemolyticum.

Antibiotic Susceptibility

  • All strains were found to be susceptible to most antibiotics tested in the study, indicating that they could be effectively treated with a variety of common antibacterial drugs.

Species Confirmation

  • Researchers confirmed the strain species by amplifying and sequencing parts of their genetic material. The sequencing of the 16S rRNA gene and the 16S-23S rRNA intergenic spacer region provided a definitive confirmation of their identity as A. haemolyticum.
  • A PCR (polymerase chain reaction) amplification technique was used to further confirm the species identity by focusing on species-specific parts of the gene encoding for phospholipase D, a common enzyme found in A. haemolyticum.

Implications for Future Identification

  • The researchers suggest that the methodology used in the study, and particularly the PCR amplification of specific parts of genes, could offer a way to improve the future identification of this usually human pathogenic bacterial species.
  • The results indicate that A. haemolyticum, usually associated with human diseases, also seems to occur in infections of horses, which could influence future research and veterinary treatment approaches.

Cite This Article

APA
Hassan AA, Ulbegi-Mohyla H, Kanbar T, Alber J, Lämmler C, Abdulmawjood A, Zschöck M, Weiss R. (2008). Phenotypic and genotypic characterization of Arcanobacterium haemolyticum isolates from infections of horses. J Clin Microbiol, 47(1), 124-128. https://doi.org/10.1128/JCM.01933-08

Publication

Journal of clinical microbiology
ISSN: 1098-660X
NlmUniqueID: 7505564
Country: United States
Language: English
Volume: 47
Issue: 1
Pages: 124-128

Researcher Affiliations

Hassan, Abdulwahed Ahmed
  • De Gezondheidsdienst voor Dieren, Postbus 9, 7400 AA Deventer, The Netherlands.
Ulbegi-Mohyla, Hivda
    Kanbar, Talah
      Alber, Jörg
        Lämmler, Christoph
          Abdulmawjood, Amir
            Zschöck, Michael
              Weiss, Reinhard

                MeSH Terms

                • Actinomycetales Infections / microbiology
                • Actinomycetales Infections / veterinary
                • Animals
                • Arcanobacterium / genetics
                • Arcanobacterium / growth & development
                • Arcanobacterium / isolation & purification
                • Arcanobacterium / metabolism
                • Bacterial Proteins / genetics
                • Bacterial Typing Techniques
                • DNA, Bacterial / chemistry
                • DNA, Bacterial / genetics
                • DNA, Ribosomal / chemistry
                • DNA, Ribosomal / genetics
                • DNA, Ribosomal Spacer / chemistry
                • DNA, Ribosomal Spacer / genetics
                • Genes, rRNA
                • Horse Diseases / microbiology
                • Horses
                • Molecular Sequence Data
                • Phospholipase D / genetics
                • Phylogeny
                • RNA, Bacterial / genetics
                • RNA, Ribosomal, 16S / genetics
                • Rabbits
                • Sequence Analysis, DNA
                • Sequence Homology, Nucleic Acid
                • Sheep

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                Citations

                This article has been cited 11 times.
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