Phloroglucinol microassay for plasma xylose in dogs and horses.

This study compares the effectiveness of the phloroglucinol microassay technique with the orcinolferric chloride technique in measuring plasma concentrations of xylose in dogs and horses, finding the former to be significantly effective.

Methodology

• Researchers compared two techniques namely the phloroglucinol microassay and the orcinolferric chloride method for measuring the plasma concentrations of xylose in animals.
• 0.5 grams of D-xylose per kilogram of body weight was administered orally to five dogs and six horses.
• After administering the dose, sequential blood samples were collected every half an hour from the test subjects.

Results

• Upon comparison of the results via regression analysis, a highly significant positive linear correlation was observed for both dogs and horses.
• The correlation coefficient for dogs was 0.95 while for horses it was 0.77. These correlation values indicate a strong agreement between the two methods.

Conclusion

• From the results, it is evident that the phloroglucinol microassay technique can be used effectively to measure xylose concentrations in the plasma of canines and equines.
• This conclusion suggests the phloroglucinol alternative might provide a less tedious approach for measuring xylose than the conventional orcinolferric chloride technique.