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Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology1996; 113(2); 221-227; doi: 10.1016/0305-0491(95)02011-x

Phospholipid composition of erythrocyte membranes and plasma of mammalian blood including Australian marsupials; quantitative 31P NMR analysis using detergent.

Abstract: The phospholipid classes of erythrocyte membranes and plasma from several domestic animals and marsupials were quantified by 31P NMR using detergents. Washed erythrocyte samples were thoroughly haemolysed by tip-sonication and dissolved in sodium cholate; plasma samples were dissolved in Triton X-100. The species studied were: common wombat (Vombatus ursinus), black-striped wallaby (Macropus dorsalis), bandicoot (Isoodon macrocarpus), Eastern grey kangaroo (Macropus giganteus), Tammar wallaby (Macropus eugenii), sheep (Ovis aries), goat (Capra hircus), cattle (Bos taurus), horse (Equus caballus), dog (Canus familiaris) and rabbit (Orytolagus caniculus). There were considerable species variations in the relative abundance of erythrocyte and plasma phospholipid classes. The variations may be attributed to the habitats and diets of the animals as well as to their phylogenetic differences.
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Publication Date: 1996-02-01 PubMed ID: 8653579DOI: 10.1016/0305-0491(95)02011-xGoogle Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study investigates the phospholipid composition found in the blood plasma and erythrocyte membranes of several mammalian species, including domestic animals and Australian marsupials, using 31P NMR analysis and detergents. The variations among these species can be attributed to their habitats, diets, and evolutionary differences.

Research Methodology

  • The researchers examined the phospholipid classes of erythrocyte membranes and plasma from several types of domestic and marsupial animals. This was conducted by using 31P NMR (Nuclear Magnetic Resonance) analysis, a method for quantifying and identifying phospholipids within the samples.
  • Target animals for the study involved a range of common domestic animals like sheep, goats, cattle, horses, dogs, and rabbits. The sampled marsupial animals were common wombats, black-striped wallabies, bandicoots, eastern grey kangaroos, and Tammar wallabies.
  • The erythrocyte samples from these animals were washed and then thoroughly haemolysed, or broken down, by tip-sonication, a method of ultrasound treatment. This process ensured a comprehensive extraction of phospholipids from the erythrocyte membranes.
  • The resultant samples were then dissolved in sodium cholate, a kind of detergent. Similarly, the plasma samples were dissolved in Triton X-100, another type of detergent. These detergents helped to prepare the samples for further analysis.

Results and Conclusion

  • The results from the analysis showed significant variations in the relative abundance of erythrocyte and plasma phospholipid classes among the studied species.
  • The differences could be attributed to various factors. Notably, the habitats and diets of the different animals could affect their biochemistry, leading to different phospholipid compositions.
  • Evolutionary differences between the studied species, termed as phylogenetic differences, were also suggested as a contributing factor to the variability of phospholipid classes.

Cite This Article

APA
Nouri-Sorkhabi MH, Agar NS, Sullivan DR, Gallagher C, Kuchel PW. (1996). Phospholipid composition of erythrocyte membranes and plasma of mammalian blood including Australian marsupials; quantitative 31P NMR analysis using detergent. Comp Biochem Physiol B Biochem Mol Biol, 113(2), 221-227. https://doi.org/10.1016/0305-0491(95)02011-x

Publication

Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
ISSN: 1096-4959
NlmUniqueID: 9516061
Country: England
Language: English
Volume: 113
Issue: 2
Pages: 221-227

Researcher Affiliations

Nouri-Sorkhabi, M H
  • Department of Biochemistry, University of Sydney, Australia.
Agar, N S
    Sullivan, D R
      Gallagher, C
        Kuchel, P W

          MeSH Terms

          • Animals
          • Australia
          • Cattle
          • Cholic Acid
          • Cholic Acids
          • Detergents
          • Dogs
          • Erythrocyte Membrane / chemistry
          • Goats
          • Horses
          • Magnetic Resonance Spectroscopy
          • Marsupialia / blood
          • Membrane Lipids / blood
          • Octoxynol
          • Phospholipids / blood
          • Phosphorus
          • Rabbits
          • Sheep

          Citations

          This article has been cited 11 times.
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            doi: 10.1007/s11259-011-9496-4pubmed: 21881904google scholar: lookup
          8. Bruschetta G, Alberghina D, Nastasi G, Rotondo E, Ferlazzo AM. Characterization of phospholipid composition of pig plasma and erythrocyte membranes. Vet Res Commun 2008 Sep;32 Suppl 1:S115-8.
            doi: 10.1007/s11259-008-9101-7pubmed: 18685996google scholar: lookup
          9. Gidalevitz D, Ishitsuka Y, Muresan AS, Konovalov O, Waring AJ, Lehrer RI, Lee KY. Interaction of antimicrobial peptide protegrin with biomembranes. Proc Natl Acad Sci U S A 2003 May 27;100(11):6302-7.
            doi: 10.1073/pnas.0934731100pubmed: 12738879google scholar: lookup
          10. Nouri-Sorkhabi MH, Wright LC, Sullivan DR, Kuchel PW. Quantitative 31P nuclear magnetic resonance analysis of the phospholipids of erythrocyte membranes using detergent. Lipids 1996 Jul;31(7):765-70.
            doi: 10.1007/BF02522893pubmed: 8827700google scholar: lookup
          11. Stott EK, Nie S, Williamson NA, Skerratt LF. Free drug percentage of moxidectin declines with increasing concentrations in the serum of marsupials. Int J Parasitol Parasites Wildl 2024 Apr;23:100899.
            doi: 10.1016/j.ijppaw.2023.100899pubmed: 38274349google scholar: lookup
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