Phospholipid composition of erythrocyte membranes and plasma of mammalian blood including Australian marsupials; quantitative 31P NMR analysis using detergent.
Abstract: The phospholipid classes of erythrocyte membranes and plasma from several domestic animals and marsupials were quantified by 31P NMR using detergents. Washed erythrocyte samples were thoroughly haemolysed by tip-sonication and dissolved in sodium cholate; plasma samples were dissolved in Triton X-100. The species studied were: common wombat (Vombatus ursinus), black-striped wallaby (Macropus dorsalis), bandicoot (Isoodon macrocarpus), Eastern grey kangaroo (Macropus giganteus), Tammar wallaby (Macropus eugenii), sheep (Ovis aries), goat (Capra hircus), cattle (Bos taurus), horse (Equus caballus), dog (Canus familiaris) and rabbit (Orytolagus caniculus). There were considerable species variations in the relative abundance of erythrocyte and plasma phospholipid classes. The variations may be attributed to the habitats and diets of the animals as well as to their phylogenetic differences.
Publication Date: 1996-02-01 PubMed ID: 8653579DOI: 10.1016/0305-0491(95)02011-xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research study investigates the phospholipid composition found in the blood plasma and erythrocyte membranes of several mammalian species, including domestic animals and Australian marsupials, using 31P NMR analysis and detergents. The variations among these species can be attributed to their habitats, diets, and evolutionary differences.
Research Methodology
- The researchers examined the phospholipid classes of erythrocyte membranes and plasma from several types of domestic and marsupial animals. This was conducted by using 31P NMR (Nuclear Magnetic Resonance) analysis, a method for quantifying and identifying phospholipids within the samples.
- Target animals for the study involved a range of common domestic animals like sheep, goats, cattle, horses, dogs, and rabbits. The sampled marsupial animals were common wombats, black-striped wallabies, bandicoots, eastern grey kangaroos, and Tammar wallabies.
- The erythrocyte samples from these animals were washed and then thoroughly haemolysed, or broken down, by tip-sonication, a method of ultrasound treatment. This process ensured a comprehensive extraction of phospholipids from the erythrocyte membranes.
- The resultant samples were then dissolved in sodium cholate, a kind of detergent. Similarly, the plasma samples were dissolved in Triton X-100, another type of detergent. These detergents helped to prepare the samples for further analysis.
Results and Conclusion
- The results from the analysis showed significant variations in the relative abundance of erythrocyte and plasma phospholipid classes among the studied species.
- The differences could be attributed to various factors. Notably, the habitats and diets of the different animals could affect their biochemistry, leading to different phospholipid compositions.
- Evolutionary differences between the studied species, termed as phylogenetic differences, were also suggested as a contributing factor to the variability of phospholipid classes.
Cite This Article
APA
Nouri-Sorkhabi MH, Agar NS, Sullivan DR, Gallagher C, Kuchel PW.
(1996).
Phospholipid composition of erythrocyte membranes and plasma of mammalian blood including Australian marsupials; quantitative 31P NMR analysis using detergent.
Comp Biochem Physiol B Biochem Mol Biol, 113(2), 221-227.
https://doi.org/10.1016/0305-0491(95)02011-x Publication
Researcher Affiliations
- Department of Biochemistry, University of Sydney, Australia.
MeSH Terms
- Animals
- Australia
- Cattle
- Cholic Acid
- Cholic Acids
- Detergents
- Dogs
- Erythrocyte Membrane / chemistry
- Goats
- Horses
- Magnetic Resonance Spectroscopy
- Marsupialia / blood
- Membrane Lipids / blood
- Octoxynol
- Phospholipids / blood
- Phosphorus
- Rabbits
- Sheep
Citations
This article has been cited 10 times.- Bogdanova A, Kaestner L. Editorial: Comparative biology of red blood cells.. Front Physiol 2022;13:1103647.
- Kumar K, Sebastiao M, Arnold AA, Bourgault S, Warschawski DE, Marcotte I. In situ solid-state NMR study of antimicrobial peptide interactions with erythrocyte membranes.. Biophys J 2022 Apr 19;121(8):1512-1524.
- Kosinska MK, Eichner G, Schmitz G, Liebisch G, Steinmeyer J. A comparative study on the lipidome of normal knee synovial fluid from humans and horses.. PLoS One 2021;16(4):e0250146.
- Toledo EB, Lucas DR, Simão TLBV, Calixto SD, Lassounskaia E, Muzitano MF, Damica FZ, Gomes VM, de Oliveira Carvalho A. Design of improved synthetic antifungal peptides with targeted variations in charge, hydrophobicity and chirality based on a correlation study between biological activity and primary structure of plant defensin γ-cores.. Amino Acids 2021 Feb;53(2):219-237.
- Jang KK, Lee ZW, Kim B, Jung YH, Han HJ, Kim MH, Kim BS, Choi SH. Identification and characterization of Vibrio vulnificus plpA encoding a phospholipase A(2) essential for pathogenesis.. J Biol Chem 2017 Oct 13;292(41):17129-17143.
- Li L, Mou X, Nelson DR. Characterization of Plp, a phosphatidylcholine-specific phospholipase and hemolysin of Vibrio anguillarum.. BMC Microbiol 2013 Nov 27;13:271.
- Ferlazzo AM, Bruschetta G, Di Pietro P, Medica P, Notti A, Rotondo E. Phospholipid composition of plasma and erythrocyte membranes in animal species by 31P NMR.. Vet Res Commun 2011 Dec;35(8):521-30.
- Bruschetta G, Alberghina D, Nastasi G, Rotondo E, Ferlazzo AM. Characterization of phospholipid composition of pig plasma and erythrocyte membranes.. Vet Res Commun 2008 Sep;32 Suppl 1:S115-8.
- Gidalevitz D, Ishitsuka Y, Muresan AS, Konovalov O, Waring AJ, Lehrer RI, Lee KY. Interaction of antimicrobial peptide protegrin with biomembranes.. Proc Natl Acad Sci U S A 2003 May 27;100(11):6302-7.
- Nouri-Sorkhabi MH, Wright LC, Sullivan DR, Kuchel PW. Quantitative 31P nuclear magnetic resonance analysis of the phospholipids of erythrocyte membranes using detergent.. Lipids 1996 Jul;31(7):765-70.
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