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Home / Equine Research Bank / J Virol / Phospholipid composition of Venezuelan equine encephalitis v...
Journal of virology1971; 7(5); 642-645; doi: 10.1128/JVI.7.5.642-645.1971

Phospholipid composition of Venezuelan equine encephalitis virus.

Abstract: Phospholipid analyses of Venezuelan equine encephalitis virus showed that virus propagated in L-cell monolayers had a higher sphingomyelin content and a lower phosphatidylcholine content than virus grown in chick fibroblast monolayers. Virus of L-cell origin also was found to possess greater thermal stability than virus derived from the chick fibroblast cell.
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Publication Date: 1971-05-01 PubMed ID: 5105003PubMed Central: PMC356174DOI: 10.1128/JVI.7.5.642-645.1971Google Scholar: Lookup
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  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the phospholipid content of the Venezuelan equine encephalitis virus, showing that the virus grown in L-cell monolayers had a different composition and stability compared to the virus grown in chick fibroblast monolayers.

Phospholipids and Virus Propagation

  • The research focuses on studying the composition of Venezuelan equine encephalitis virus, a mosquito-borne viral pathogen causing fatal disease in humans and equines.
  • The central interest is the phospholipid content of the virus, as phospholipids play an important role in the survival and function of the virus.

L-cell and Chick Fibroblast Monolayers

  • Two different types of cell monolayers were used in the research: L-cell monolayers and chick fibroblast monolayers.
  • L-cell monolayers are cultures derived from mouse connective tissue cells, while chick fibroblast monolayers are cultures from the cells in young chicken fibres.

Phospholipid Content Differences

  • The researchers discovered that the Venezuelan equine encephalitis virus propagated in L-cell monolayers had a higher content of sphingomyelin and a lower content of phosphatidylcholine compared to the virus grown in chick fibroblast monolayers.
  • Sphingomyelin and phosphatidylcholine are types of phospholipids, which are important components of cell membranes and contribute to the properties of viruses.
  • The differences in phospholipid content suggest that the virus adapts its composition based on the type of cells it infects.

Thermal Stability Differences

  • In addition, it was found that the virus of L-cell origin demonstrated greater thermal stability than the virus derived from chick fibroblast cells.
  • Thermal stability of a virus refers to its ability to retain its infectious properties under varying temperature conditions. A higher thermal stability indicates a higher resilience of the virus under temperature fluctuations.
  • The findings of greater thermal stability in virus from L-cell monolayers could have implications for how the virus survives in different environments and hosts.

Conclusions and Implications

  • The study provides detailed insights into the phospholipid composition in Venezuelan equine encephalitis virus when it infects different types of cells.
  • The variations in virus phospholipid composition and thermal stability may affect virus infectivity, survival, and the manifestation of disease.
  • These findings are potentially significant for further understanding how the Venezuelan equine encephalitis virus infects host cells, with potential implications for treatment and prevention strategies.

Cite This Article

APA
Heydrick FP, Comer JF, Wachter RF. (1971). Phospholipid composition of Venezuelan equine encephalitis virus. J Virol, 7(5), 642-645. https://doi.org/10.1128/JVI.7.5.642-645.1971

Publication

Journal of virology
ISSN: 0022-538X
NlmUniqueID: 0113724
Country: United States
Language: English
Volume: 7
Issue: 5
Pages: 642-645

Researcher Affiliations

Heydrick, F P
    Comer, J F
      Wachter, R F

        MeSH Terms

        • Animals
        • Chick Embryo
        • Culture Techniques
        • Encephalitis Viruses / analysis
        • Fibroblasts
        • L Cells
        • Phospholipids / analysis
        • Virus Cultivation

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        This article includes 15 references
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        Citations

        This article has been cited 3 times.
        1. Petratos S, Gonzales ME. Can antiglycolipid antibodies present in HIV-infected individuals induce immune demyelination?. Neuropathology 2000 Dec;20(4):257-72.
          doi: 10.1046/j.1440-1789.2000.00356.xpubmed: 11211050google scholar: lookup
        2. Webb HE, Fazakerley JK. Can viral envelope glycolipids produce auto-immunity, with reference to the CNS and multiple sclerosis?. Neuropathol Appl Neurobiol 1984 Jan-Feb;10(1):1-10.
          doi: 10.1111/j.1365-2990.1984.tb00335.xpubmed: 6330601google scholar: lookup
        3. Lenard J, Compans RW. The membrane structure of lipid-containing viruses.. Biochim Biophys Acta 1974 Apr 8;344(1):51-94.
          doi: 10.1016/0304-4157(74)90008-2pubmed: 4598854google scholar: lookup
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