FREE SHIPPING over $40
OVER 10000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Journal of virology1971; 7(5); 642-645; doi: 10.1128/JVI.7.5.642-645.1971

Phospholipid composition of Venezuelan equine encephalitis virus.

Abstract: Phospholipid analyses of Venezuelan equine encephalitis virus showed that virus propagated in L-cell monolayers had a higher sphingomyelin content and a lower phosphatidylcholine content than virus grown in chick fibroblast monolayers. Virus of L-cell origin also was found to possess greater thermal stability than virus derived from the chick fibroblast cell.
Get Full Text
Publication Date: 1971-05-01 PubMed ID: 5105003PubMed Central: PMC356174DOI: 10.1128/JVI.7.5.642-645.1971Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the phospholipid content of the Venezuelan equine encephalitis virus, showing that the virus grown in L-cell monolayers had a different composition and stability compared to the virus grown in chick fibroblast monolayers.

Phospholipids and Virus Propagation

  • The research focuses on studying the composition of Venezuelan equine encephalitis virus, a mosquito-borne viral pathogen causing fatal disease in humans and equines.
  • The central interest is the phospholipid content of the virus, as phospholipids play an important role in the survival and function of the virus.

L-cell and Chick Fibroblast Monolayers

  • Two different types of cell monolayers were used in the research: L-cell monolayers and chick fibroblast monolayers.
  • L-cell monolayers are cultures derived from mouse connective tissue cells, while chick fibroblast monolayers are cultures from the cells in young chicken fibres.

Phospholipid Content Differences

  • The researchers discovered that the Venezuelan equine encephalitis virus propagated in L-cell monolayers had a higher content of sphingomyelin and a lower content of phosphatidylcholine compared to the virus grown in chick fibroblast monolayers.
  • Sphingomyelin and phosphatidylcholine are types of phospholipids, which are important components of cell membranes and contribute to the properties of viruses.
  • The differences in phospholipid content suggest that the virus adapts its composition based on the type of cells it infects.

Thermal Stability Differences

  • In addition, it was found that the virus of L-cell origin demonstrated greater thermal stability than the virus derived from chick fibroblast cells.
  • Thermal stability of a virus refers to its ability to retain its infectious properties under varying temperature conditions. A higher thermal stability indicates a higher resilience of the virus under temperature fluctuations.
  • The findings of greater thermal stability in virus from L-cell monolayers could have implications for how the virus survives in different environments and hosts.

Conclusions and Implications

  • The study provides detailed insights into the phospholipid composition in Venezuelan equine encephalitis virus when it infects different types of cells.
  • The variations in virus phospholipid composition and thermal stability may affect virus infectivity, survival, and the manifestation of disease.
  • These findings are potentially significant for further understanding how the Venezuelan equine encephalitis virus infects host cells, with potential implications for treatment and prevention strategies.

Cite This Article

APA
Heydrick FP, Comer JF, Wachter RF. (1971). Phospholipid composition of Venezuelan equine encephalitis virus. J Virol, 7(5), 642-645. https://doi.org/10.1128/JVI.7.5.642-645.1971

Publication

Journal of virology
ISSN: 0022-538X
NlmUniqueID: 0113724
Country: United States
Language: English
Volume: 7
Issue: 5
Pages: 642-645

Researcher Affiliations

Heydrick, F P
    Comer, J F
      Wachter, R F

        MeSH Terms

        • Animals
        • Chick Embryo
        • Culture Techniques
        • Encephalitis Viruses / analysis
        • Fibroblasts
        • L Cells
        • Phospholipids / analysis
        • Virus Cultivation

        References

        This article includes 15 references
        1. Heydrick FP, Wachter RF, Hearn HJ Jr. Host influence on the characteristics of Venezuelan equine encephalomyelitis virus.. J Bacteriol 1966 Jun;91(6):2343-8.
          pubmed: 5943943doi: 10.1128/jb.91.6.2343-2348.1966google scholar: lookup
        2. Simpson RW, Hauser RE. Influence of lipids on the viral phenotype. I. Interaction of myxoviruses and their lipid constituents with phospholipases.. Virology 1966 Dec;30(4):684-97.
          pubmed: 5928796doi: 10.1016/0042-6822(66)90173-5google scholar: lookup
        3. Acheson NH, Tamm I. Replication of Semliki Forest virus: an electron microscopic study.. Virology 1967 May;32(1):128-43.
          pubmed: 6067298doi: 10.1016/0042-6822(67)90261-9google scholar: lookup
        4. Wells MA, Dittmer JC. A microanalytical technique for the quantitative determination of twenty-four classes of brain lipids.. Biochemistry 1966 Nov;5(11):3405-18.
          pubmed: 5972320doi: 10.1021/bi00875a004google scholar: lookup
        5. Strauss JH Jr, Burge BW, Pfefferkorn ER, Darnell JE Jr. Identification of the membrane protein and "core" protein of Sindbis virus.. Proc Natl Acad Sci U S A 1968 Feb;59(2):533-7.
          pubmed: 5238983doi: 10.1073/pnas.59.2.533google scholar: lookup
        6. Weinstein DB, Marsh JB, Glick MC, Warren L. Membranes of animal cells. IV. Lipids of the L cell and its surface membrane.. J Biol Chem 1969 Aug 10;244(15):4103-11.
          pubmed: 4308167
        7. Friedman RM, Pastan I. Nature and function of the structural phospholipids of an arbovirus.. J Mol Biol 1969 Feb 28;40(1):107-15.
          pubmed: 5391555doi: 10.1016/0022-2836(69)90299-xgoogle scholar: lookup
        8. McSharry JJ, Wagner RR. Lipid composition of purified vesicular stomatitis viruses.. J Virol 1971 Jan;7(1):59-70.
          pubmed: 5101092doi: 10.1128/JVI.7.1.59-70.1971google scholar: lookup
        9. Burge BW, Huang AS. Comparison of membrane protein glycopeptides of Sindbis virus and vesicular stomatitis virus.. J Virol 1970 Aug;6(2):176-82.
          pubmed: 4322870doi: 10.1128/JVI.6.2.176-182.1970google scholar: lookup
        10. ANDERSON SG, ADA GL. The action of phospholipase A and lipid solvents on Murray Valley encephalitis virus.. J Gen Microbiol 1961 Jul;25:451-8.
          pubmed: 13683281doi: 10.1099/00221287-25-3-451google scholar: lookup
        11. MORGAN C, HOWE C, ROSE HM. Structure and development of viruses as observed in the electron microscope. V. Western equine encephalomyelitis virus.. J Exp Med 1961 Jan 1;113(1):219-34.
          pubmed: 13772566doi: 10.1084/jem.113.1.219google scholar: lookup
        12. COLON JI, IDOINE JB. FACTORS AFFECTING PLAQUE FORMATION BY THE INFECTIOUS RIBONUCLEIC ACID OF THE EQUINE ENCEPHALITIS VIRUSES.. J Infect Dis 1964 Feb;114:61-8.
          pubmed: 14118050doi: 10.1093/infdis/114.1.61google scholar: lookup
        13. PFEFFERKORN ER, HUNTER HS. PURIFICATION AND PARTIAL CHEMICAL ANALYSIS OF SINDBIS VIRUS.. Virology 1963 Jul;20:433-45.
          pubmed: 14043407doi: 10.1016/0042-6822(63)90092-8google scholar: lookup
        14. PFEFFERKORN ER, HUNTER HS. THE SOURCE OF THE RIBONUCLEIC ACID AND PHOSPHOLIPID OF SINDBIS VIRUS.. Virology 1963 Jul;20:446-56.
          pubmed: 14043408doi: 10.1016/0042-6822(63)90093-xgoogle scholar: lookup
        15. BENSON AA, STRICKLAND EH. Plant phospholipids. 3. Identification of diphosphatidyl glycerol.. Biochim Biophys Acta 1960 Jul 1;41:328-33.
          pubmed: 13798992doi: 10.1016/0006-3002(60)90016-0google scholar: lookup

        Citations

        This article has been cited 4 times.
        1. Petratos S, Gonzales ME. Can antiglycolipid antibodies present in HIV-infected individuals induce immune demyelination?. Neuropathology 2000 Dec;20(4):257-72.
          doi: 10.1046/j.1440-1789.2000.00356.xpubmed: 11211050google scholar: lookup
        2. Webb HE, Fazakerley JK. Can viral envelope glycolipids produce auto-immunity, with reference to the CNS and multiple sclerosis?. Neuropathol Appl Neurobiol 1984 Jan-Feb;10(1):1-10.
          doi: 10.1111/j.1365-2990.1984.tb00335.xpubmed: 6330601google scholar: lookup
        3. Lenard J, Compans RW. The membrane structure of lipid-containing viruses. Biochim Biophys Acta 1974 Apr 8;344(1):51-94.
          doi: 10.1016/0304-4157(74)90008-2pubmed: 4598854google scholar: lookup
        4. Blough HA, Tiffany JM. Lipids in viruses. Adv Lipid Res 1973;11:267-339.
          doi: 10.1016/B978-0-12-024911-4.50014-3pubmed: 4354501google scholar: lookup
        Subscribe to our newsletter
        Join 99,780 horse owners like you who receive our email updates on horse health and nutrition.