Phospholipid compositions of sera and synovial fluids from dog, human and horse: a comparison by 31P-NMR and MALDI-TOF MS.
Abstract: Alterations of the phospholipid (PL) compositions of body fluids are assumed to be indicative of inflammatory diseases, e.g. rheumatoid arthritis (RA). Recently, we have shown that particularly the phosphatidylcholine/lysophosphatidylcholine (PC/LPC) ratio determined in human synovial fluids (SF) and sera represents a reliable measure of the inflammatory state in RA patients. However, it is not yet clear to what extent the PC/LPC ratio is also affected by nutrition habits. In the present study, the PL and the corresponding acyl chain compositions of human body fluids (SF and serum of RA patients as well as serum from healthy volunteers) are compared with those of two other mammalian species (horses and dogs suffering from degenerative joint diseases as well as healthy controls) by high-resolution 31P-nuclear magnetic resonance (NMR) spectroscopy and matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS). The most important result of this study is that the PL compositions of SF and serum of horse and dog are comparable with those of human body fluids. Compared with humans, however, the horse body fluid contains less PCs with highly unsaturated arachidonoyl residues, while that of dogs possesses the highest content of arachidonoyl-containing PC. These species-related differences stem primarily from different nutrition habits (meat vs. plants).
Publication Date: 2008-05-20 PubMed ID: 18484967DOI: 10.1111/j.1439-0396.2008.00822.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research investigates the differences in phospholipid composition in the body fluids of humans, horses and dogs, in both healthy states and during inflammatory conditions. The study further explores the influence of dietary habits on these compositions, specifically those involved in rheumatoid arthritis (RA) conditions.
Overview of the Research
- The study was developed to understand the phospholipid (PL) compositions within the body fluids of various mammals. The focus was primarily on synovial fluids (SF) and sera (the clear portion of blood) in the contexts of normal health and inflammation.
- A specific focus was placed on the ratio of phosphatidylcholine/lysophosphatidylcholine (PC/LPC), which has been shown in previous studies to be indicative of inflammatory states in humans, particularly those with rheumatoid arthritis (RA).
- This research looked to further these investigations by comparing the PL and acyl chain compositions of the body fluids of humans (both with RA and without) to those of two other mammalian species – horses and dogs.
- Both the animals with healthy conditions and those suffering from degenerative joint diseases were considered in the comparison.
Methods
- In order to conduct these comparisons, the researchers used high-resolution 31P-nuclear magnetic resonance (31P-NMR) spectroscopy and matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS).
Findings
- The outcomes indicated comparable phospholipid compositions across human, horse and dog body fluids.
- However, significant differences were observed in the PCs with highly unsaturated arachidonoyl residues. Humans had more of these, compared to the horses, while dogs showed the highest content of arachidonoyl-containing PC.
- These differences have been mainly attributed to the distinct nutritional habits across species (meat-centric diet in dogs versus plant-based in horses).
Implications
- This study provides a foundation for understanding how comparative biology and nutrition can influence phospholipid compositions in the body’s fluids and thus contribute to the progression of inflammatory diseases like rheumatoid arthritis.
- The study’s results imply that diet might play an essential role in maintaining or disrupting the balance of phospholipid compositions, potentially affecting the individual’s susceptibility to related inflammatory diseases.
- This research can provide insights for future studies that aim to understand the influence of diet in managing diseases related to inflammation.
Cite This Article
APA
Fuchs B, Bondzio A, Wagner U, Schiller J.
(2008).
Phospholipid compositions of sera and synovial fluids from dog, human and horse: a comparison by 31P-NMR and MALDI-TOF MS.
J Anim Physiol Anim Nutr (Berl), 93(4), 410-422.
https://doi.org/10.1111/j.1439-0396.2008.00822.x Publication
Researcher Affiliations
- Institute of Medical Physics and Biophysics, Medical Department, University of Leipzig, Leipzig, Germany. beate.fuchs@medizin.uni-leipzig.de
MeSH Terms
- Adult
- Animals
- Dogs
- Female
- Horses / blood
- Humans
- Magnetic Resonance Spectroscopy / methods
- Male
- Middle Aged
- Phospholipids / analysis
- Phospholipids / blood
- Species Specificity
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
- Synovial Fluid / chemistry
Citations
This article has been cited 9 times.- Steinmeyer J. Phospholipids and Sphingolipids in Osteoarthritis. Biomolecules 2025 Feb 8;15(2).
- Mustonen AM, Lehmonen N, Paakkonen T, Raekallio M, Käkelä R, Niemelä T, Mykkänen A, Sihvo SP, Nieminen P. Equine osteoarthritis modifies fatty acid signatures in synovial fluid and its extracellular vesicles. Arthritis Res Ther 2023 Mar 9;25(1):39.
- Bartikoski BJ, De Oliveira MS, Do Espírito Santo RC, Dos Santos LP, Dos Santos NG, Xavier RM. A Review of Metabolomic Profiling in Rheumatoid Arthritis: Bringing New Insights in Disease Pathogenesis, Treatment and Comorbidities. Metabolites 2022 Apr 27;12(5).
- Burk J, Melzer M, Hagen A, Lips KS, Trinkaus K, Nimptsch A, Leopold J. Phospholipid Profiles for Phenotypic Characterization of Adipose-Derived Multipotent Mesenchymal Stromal Cells. Front Cell Dev Biol 2021;9:784405.
- Kosinska MK, Eichner G, Schmitz G, Liebisch G, Steinmeyer J. A comparative study on the lipidome of normal knee synovial fluid from humans and horses. PLoS One 2021;16(4):e0250146.
- Valueva AV, Romanov RS, Mariasina SS, Eliseev MS, Rodina EV. Inorganic Pyrophosphatase-Nanodiamond Conjugates Hydrolyze Pyrophosphate in Human Synovial Fluid. ACS Omega 2020 Apr 21;5(15):8579-8586.
- Hallamaa R, Batchu K. Phospholipid analysis in sera of horses with allergic dermatitis and in matched healthy controls. Lipids Health Dis 2016 Mar 2;15:45.
- Giera M, Ioan-Facsinay A, Toes R, Gao F, Dalli J, Deelder AM, Serhan CN, Mayboroda OA. Lipid and lipid mediator profiling of human synovial fluid in rheumatoid arthritis patients by means of LC-MS/MS. Biochim Biophys Acta 2012 Nov;1821(11):1415-24.
- Ferlazzo AM, Bruschetta G, Di Pietro P, Medica P, Notti A, Rotondo E. Phospholipid composition of plasma and erythrocyte membranes in animal species by 31P NMR. Vet Res Commun 2011 Dec;35(8):521-30.
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