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Home / Equine Research Bank / Sci Rep / Phosphorylation of SNW1 protein associated with equine melan...
Scientific reports2024; 14(1); 30842; doi: 10.1038/s41598-024-81338-6

Phosphorylation of SNW1 protein associated with equine melanocytic neoplasm identified in serum and feces.

Abstract: Equine melanocytic neoplasm (EMN) represents a form of skin tumor observed predominantly in grey horses aged over 15 years. Despite its prevalence, current therapeutic and preventive strategies for EMN have been subject to limited investigation. This study endeavors to shed light on potential phosphoproteins present in equine serum and fecal samples, potentially linked to EMN, with a specific focus on functional interactions in EMN pathogenesis. We examined 50 samples (25 serum, 25 feces), divided into three groups based on EMN severity: normal (n = 16), mild (n = 18), and severe EMN (n = 16). Equine phosphoproteome analysis identified 2,359 annotated serum phosphoproteins and 2002 annotated fecal phosphoproteins through differentially expressed proteins (DEPs). KEGG analysis emphasized the role of environmental information processing. Notably, the integrin NF-kappaB binding P-TEFb to stimulate transcriptional elongation signaling pathway, involving SNW1 protein, was implicated in early stage of EMN development in both serum and fecal samples. This highlights SNW1's potential role in mediating transcriptional processes, offering a novel marker within environmental information processing. This study enhances understanding of EMN mechanisms in horses, suggesting early detection through non-invasive methods and identifying a functional pathway involving SNW1, which could inform future treatment and prevention strategies.
© 2024. The Author(s).
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Publication Date: 2024-12-28 PubMed ID: 39730520PubMed Central: PMC11680861DOI: 10.1038/s41598-024-81338-6Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explored potential proteins linked with equine melanocytic neoplasm (EMN) – a form of skin tumor common in older grey horses – in serum and fecal samples of horses with varying degrees of the disease. The study found important proteins that seem to play a role in the development of the disease, most notably SNW1, suggesting the possibility of early, non-invasive detection methods and new directions for treatment strategies.

Study Background and Objectives

  • The main objective of this study was to identify potentially significant phosphoproteins present in the serum and feces of horses that have some connection with equine melanocytic neoplasm (EMN).
  • The aim was to provide improved understanding on the disease mechanisms of EMN, which is a prevalent condition among grey horses over 15 years old but had not been the subject of extensive research to date.

Research Methodology

  • 50 samples (25 each from serum and feces) were examined for this study, with subjects divided into three groups according to the severity of EMN – normal, mild, and severe.
  • Through the analysis of differentially expressed proteins (DEPs), the team identified 2,359 serum phosphoproteins and 2002 fecal phosphoproteins relevant to the study.
  • The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was used to underscore the importance of environmental information processing within the identified proteins.

Key Findings and Potential Implications

  • A key signaling pathway involving the SNW1 protein was implicated at an early stage of EMN development in both serum and fecal samples.
  • This suggests that SNW1 plays a crucial role in the transcriptional processes related to the early development of EMN.
  • The study’s findings potentially present SNW1 as a promising marker for early detection of EMN through non-invasive methods and could lead to novel strategies for treatment and prevention of the disease.
  • Furthermore, the results of the study contribute to a better understanding of how EMN develops and operates, and could guide future research and therapeutic interventions for this common equine disease.

Cite This Article

APA
Vinijkumthorn R, Kingkaw A, Yanyongsirikarn P, Phaonakrop N, Roytrakul S, Vongsangnak W, Tesena P. (2024). Phosphorylation of SNW1 protein associated with equine melanocytic neoplasm identified in serum and feces. Sci Rep, 14(1), 30842. https://doi.org/10.1038/s41598-024-81338-6

Publication

Scientific reports
ISSN: 2045-2322
NlmUniqueID: 101563288
Country: England
Language: English
Volume: 14
Issue: 1
Pages: 30842
PII: 30842

Researcher Affiliations

Vinijkumthorn, Ruethaiwan
  • Department of Clinical Science and Public Health, Faculty of Veterinary Science, Mahidol University, Salaya, Puttamonthon, Nakhon Pathom, 73170, Thailand.
Kingkaw, Amornthep
  • Interdisciplinary Graduate Program in Bioscience, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand.
Yanyongsirikarn, Petchpailin
  • Prasuarthon Small Animal Hospital, Faculty of Veterinary Science, Equine Clinic, Mahidol University, Salaya, Puttamonthon, Nakhon Pathom, 73170, Thailand.
Phaonakrop, Narumon
  • Functional Proteomics Technology Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand.
Roytrakul, Sittiruk
  • Functional Proteomics Technology Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand.
Vongsangnak, Wanwipa
  • Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand. wanwipa.v@ku.ac.th.
  • Omics Center for Agriculture, Bioresources, Food, and Health, Kasetsart University (OmiKU), Bangkok, 10900, Thailand. wanwipa.v@ku.ac.th.
Tesena, Parichart
  • Department of Clinical Science and Public Health, Faculty of Veterinary Science, Mahidol University, Salaya, Puttamonthon, Nakhon Pathom, 73170, Thailand. parichart.tes@mahidol.edu.

MeSH Terms

  • Horses
  • Animals
  • Feces / chemistry
  • Horse Diseases / metabolism
  • Horse Diseases / blood
  • Skin Neoplasms / veterinary
  • Skin Neoplasms / metabolism
  • Skin Neoplasms / blood
  • Skin Neoplasms / pathology
  • Phosphorylation
  • Phosphoproteins / metabolism
  • Biomarkers, Tumor / blood
  • Biomarkers, Tumor / metabolism
  • Melanoma / metabolism
  • Melanoma / veterinary
  • Melanoma / blood

Grant Funding

  • MU-SRF-RS-14A/66 / Mahidol University (MU's Strategic Research Fund): 2023
  • MU-SRF-RS-14A/66 / Mahidol University (MU's Strategic Research Fund): 2023
  • MU-SRF-RS-14A/66 / Mahidol University (MU's Strategic Research Fund): 2023
  • MU-SRF-RS-14A/66 / Mahidol University (MU's Strategic Research Fund): 2023
  • MU-SRF-RS-14A/66 / Mahidol University (MU's Strategic Research Fund): 2023
  • MU-SRF-RS-14A/66 / Mahidol University (MU's Strategic Research Fund): 2023
  • MU-SRF-RS-14A/66 / Mahidol University (MU's Strategic Research Fund): 2023

Conflict of Interest Statement

Declarations. Competing interests: The authors declare no competing interests. Ethics approval: All methods were carried out in accordance with relevant guidelines and regulation, and the study was performed in compliance with the ARRIVE guidelines. The research ethics was approved by the Faculty of Veterinary Science, Mahidol University-Institute Animal Care and Use Committee (FVS-MU-IACUC-Protocol No. MUVS-2020-12-62), Animal use license No. U1-3498-2545.

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