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Animal genetics1999; 30(2); 102-108; doi: 10.1046/j.1365-2052.1999.00419.x

Phylogenetic relationships of Cheju horses to other horse breeds as determined by mtDNA D-loop sequence polymorphism.

Abstract: Historical records suggest that horses inhabiting the island of Cheju in Korea are descendants of Mongolian horses introduced in 1276. Other studies, however, suggest that horses may have been present on the island prior to the Mongolian introduction. To determine the origin of the Cheju horses we used a phylogenetic analysis of sequences of the mitochondrial DNA (mtDNA) D-loop region, including tRNA Pro and parts of tRNA thr and tRNA Phe sequences (1102-bp excluding the tandem repeat region). Maximum parsimony and neighbor-joining trees were constructed using sequences determined for seven Cheju, four Mongolian, one Przewalskii and two Chinese Yunnan horses, and published sequences for one Swedish and three Thoroughbred horses. Donkey mtDNA was used as an outgroup. We found that the mtDNA D-loop sequence varies considerably within Mongolian, Cheju and Thoroughbred horse breeds, and that Cheju horses clustered with Mongolian horses as well as with horses from other distantly related breeds. On the basis of these findings we propose that horses on Cheju Island are of mixed origin in their maternal lineage, and that horses may have existed and been traded on the island before the Mongolian introduction.
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Publication Date: 1999-06-22 PubMed ID: 10376300DOI: 10.1046/j.1365-2052.1999.00419.xGoogle Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates the origins of Cheju horses from Korea, using the comparative DNA analysis among various horse breeds. The findings suggest the Cheju horses probably have a mixed heritage, with evidence of ancestral connections to both Mongolian horses and other diverse breeds.

Background

  • This research is centred around understanding the genetic ancestry of Cheju horses, a breed native to the Cheju island in Korea. Historically, it was considered that these horses descended from Mongolian horses introduced to the island in the 13th century, but there have been challenges to this idea, with suggestions that horses may have been present on the island prior to that time.

Methods

  • The researchers used a phylogenetic analysis, a technique commonly used for understanding hereditary relationships, to trace potential ancestry. Specifically, they analysed the mitochondrial DNA (mtDNA), or the DNA passed down from the mother, in the D-loop region, which is highly variable and can therefore provide detailed insights about genetic differences among animals.
  • The breeds used for comparison included seven Cheju horses, four Mongolian horses, one Przewalskii horse, two Chinese Yunnan horses, and one Swedish and three Thoroughbred horses. Donkey mtDNA was used as an outgroup for clarity in results.
  • The data was then compiled into trees using two commonly used constructions for phylogenetic trees – the maximum parsimony and neighbor-joining trees – to provide a visual, branch-like structure of potential common ancestors and points of divergence among breeds.

Results and Conclusion

  • The results showed considerable variation in the mtDNA D-loop sequence within Mongolian, Cheju, and Thoroughbred horse breeds. Interestingly, the Cheju horses were found to be genetically linked not only to Mongolian horses but also to other diverse and distantly related breeds.
  • Based on these findings, the researchers propose that Cheju horses likely have a mixed origin in their maternal lineage. This adds weight to the suggestions that horses were present and even traded on Cheju Island before the known Mongolian introduction in the 13th century.

Cite This Article

APA
Kim KI, Yang YH, Lee SS, Park C, Ma R, Bouzat JL, Lewin HA. (1999). Phylogenetic relationships of Cheju horses to other horse breeds as determined by mtDNA D-loop sequence polymorphism. Anim Genet, 30(2), 102-108. https://doi.org/10.1046/j.1365-2052.1999.00419.x

Publication

Animal genetics
ISSN: 0268-9146
NlmUniqueID: 8605704
Country: England
Language: English
Volume: 30
Issue: 2
Pages: 102-108

Researcher Affiliations

Kim, K I
  • Department of Animal Biotechnology, Cheju National University, South Korea.
Yang, Y H
    Lee, S S
      Park, C
        Ma, R
          Bouzat, J L
            Lewin, H A

              MeSH Terms

              • Animals
              • Base Sequence
              • Cluster Analysis
              • DNA Primers / chemistry
              • DNA, Mitochondrial / chemistry
              • Female
              • Fossils
              • Horses / genetics
              • Korea
              • Molecular Sequence Data
              • Phylogeny
              • Polymerase Chain Reaction / veterinary
              • Polymorphism, Restriction Fragment Length
              • Sequence Alignment / veterinary
              • Sequence Analysis, DNA
              • Sequence Homology, Nucleic Acid

              Citations

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