Phylogeny of immune recognition: processing and presentation of structurally defined proteins in channel catfish immune responses.
Abstract: This work was undertaken to investigate whether or not antigen processing and presentation are important in channel catfish in vitro secondary immune responses elicited with structurally defined proteins, namely, pigeon heart cytochrome C (pCytC), hen egg lysozyme, and horse myoglobin. The use of in vitro antigen-pulsed and fixed B cells or monocytes as antigen presenting cells (APC) resulted in autologous peripheral blood leukocytes (PBL) responding with vigorous proliferation and antibody production in vitro. In addition, several long-term catfish monocyte lines have been found to function as efficient APC with autologous but not allogeneic responders. Subsequent separation of the responding PBL into sIg- (T-cell-enriched) and B (sIg+) cells subsets showed that both underwent proliferative responses to antigen-pulsed and fixed APC. Moreover, allogeneic cells used as APC were found to induce only strong mixed leukocyte reactions without specific in vitro antibody production. Initial attempts at identifying the immunogenic region(s) of the protein antigens for catfish indicated there are two such regions for pCytC, namely, peptides 66-80 and 81-104.
Publication Date: 1991-01-01 PubMed ID: 1668258PubMed Central: PMC2275833DOI: 10.1155/1991/32534Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- U.S. Gov't
- P.H.S.
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research indicates that antigen processing and presentation play a significant role in the secondary immune responses of channel catfish in response to structurally defined proteins.
Objectives and Approach
- The study was conducted to explore the role of antigen processing and presentation in triggering the secondary immune responses in channel catfish when they are exposed to specific proteins, such as pigeon heart cytochrome C (pCytC), hen egg lysozyme, and horse myoglobin.
- They used antigen-stimulated and fixed B cells or monocytes as antigen presenting cells (APCs), and it was observed that peripheral blood leukocytes (PBLs) from the same source (autologous) responded with a significant increase in cell growth and antibody production.
Key Findings
- Several long-term catfish monocyte cell lines were found to function as efficient APCs with autologous responses, but not with allogeneic (coming from a different individual) reactions.
- The responding PBLs were divided into sIg- (T-cell-enriched) and B (sIg+) cells subsets, and it was found that both showed proliferative responses to antigen-stimulated and fixed APCs.
- For APCs of allogeneic origin, they induced strong mixed leukocyte reactions, but did not trigger specific in vitro antibody production. This shows APCs from a different source than the responder cells are unable to stimulate specific immune responses.
- Initial identification of the immunogenic regions of the protein antigens for catfish showed that there were two for pCytC, namely, peptides 66-80 and 81-104. These areas are responsible for invoking an immune response.
Conclusions
- This research suggests that antigen processing and presentation play a crucial role in the immune responses of channel catfish, leading to increased cell proliferation and antibody production.
- It also specifies the immunogenic regions on the protein antigens for catfish, which can be critical information for further research on improving the immunity of these species.
Cite This Article
APA
Vallejo AN, Miller NW, Clem LW.
(1991).
Phylogeny of immune recognition: processing and presentation of structurally defined proteins in channel catfish immune responses.
Dev Immunol, 1(3), 137-148.
https://doi.org/10.1155/1991/32534 Publication
Researcher Affiliations
- Department of Microbiology, University of Mississippi Medical Center, Jackson 39216-4505.
MeSH Terms
- Animals
- Antibody Formation
- Antigen-Presenting Cells / immunology
- Antigen-Presenting Cells / metabolism
- Antigens / immunology
- Antigens / metabolism
- Cell Line
- Chickens / immunology
- Columbidae / immunology
- Cytochrome c Group / immunology
- Horses / immunology
- Ictaluridae / immunology
- Immunization
- Lymphocyte Activation
- Lymphocyte Culture Test, Mixed
- Lymphocytes / immunology
- Monocytes / immunology
- Muramidase / immunology
- Myoglobin / immunology
- Peptide Fragments / immunology
- Phylogeny
- Species Specificity
Grant Funding
- 5-R37-AI-19530 / NIAID NIH HHS
Citations
This article has been cited 2 times.- Bassity E, Clark TG. Functional identification of dendritic cells in the teleost model, rainbow trout (Oncorhynchus mykiss). PLoS One 2012;7(3):e33196.
- Vallejo AN, Miller NW, Clem LW. Phylogeny of immune recognition: role of alloantigens in antigen presentation in channel catfish immune responses. Immunology 1991 Sep;74(1):165-8.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
