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Home / Equine Research Bank / Arch Virol / Physical mapping of the genomic heterogeneity of isolates of...
Archives of virology1989; 104(1-2); 87-94; doi: 10.1007/BF01313810

Physical mapping of the genomic heterogeneity of isolates of equine herpesvirus 2 (equine cytomegalovirus).

Abstract: The BamHI, EcoRI, and HindIII physical maps of the genomes of 14 isolates of equine herpesvirus 2 (EHV 2) were determined by Southern blot analysis using DNA fragments of a previously mapped EHV 2 strain 86/67. No two isolates had identical maps for all 3 enzymes, the number of differing cleavage sites between pairs of isolates varying from 3 to 21. Overall 75 cleavage sites were mapped, of which 40 were variable. Cleavage sites occurred throughout the genome, including within the terminal repeat regions. Additionally, fragment length polymorphisms, independent of cleavage site loss or gain, were mapped to 5 regions of the genome, 4 of which occurred within the terminal repeat regions.
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Publication Date: 1989-01-01 PubMed ID: 2564271DOI: 10.1007/BF01313810Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigated the genetic diversity among 14 isolates of the equine herpesvirus 2 (EHV 2) through physical mapping using three different enzymes. The study revealed significant genomic heterogeneity within EHV 2 isolates, which manifested in variable cleavage sites and fragment length polymorphisms throughout the genome, including the terminal repeat regions.

Physical Mapping of EHV 2 Isolates

  • Using Southern blot analysis, the genomic physical maps of 14 isolates of EHV 2 were examined.
  • The enzymes used in this process were BamHI, EcoRI, and HindIII.
  • For comparison, the DNA fragments of a previously mapped EHV 2 strain 86/67 were used.
  • The study found that none of the isolates had identical maps for all three enzymes, indicating significant genomic variation.

Genomic Heterogeneity Observed

  • The heterogeneity was evident in the varying number of differing cleavage sites between pairs of isolates, which ranged from 3 to 21.
  • Altogether, 75 cleavage sites were mapped out of which 40 were found to be variable.
  • This suggests a high degree of variability among the EHV 2 isolates at the genomic level.

Terminal Repeat Regions Variability

  • Interestingly, the cleavage sites occurred all over the genome, including the terminal repeat regions.
  • Fragment length polymorphisms, independent of cleavage site loss or gain, were also discovered.
  • These polymorphisms were mapped to five regions of the genome, four of which occurred within the terminal repeat regions.

Conclusion

  • The research provides valuable insights into the genomic diversity of EHV 2 isolates.
  • Intensive understanding of this genomic heterogeneity could be critical for the development of effective diagnostic tools and vaccines against the equine herpesvirus 2.

Cite This Article

APA
Browning GF, Studdert MJ. (1989). Physical mapping of the genomic heterogeneity of isolates of equine herpesvirus 2 (equine cytomegalovirus). Arch Virol, 104(1-2), 87-94. https://doi.org/10.1007/BF01313810

Publication

Archives of virology
ISSN: 0304-8608
NlmUniqueID: 7506870
Country: Austria
Language: English
Volume: 104
Issue: 1-2
Pages: 87-94

Researcher Affiliations

Browning, G F
  • School of Veterinary Science, University of Melbourne, Parkville, Victoria, Australia.
Studdert, M J

    MeSH Terms

    • Genes, Viral
    • Herpesviridae / genetics
    • Polymorphism, Genetic
    • Polymorphism, Restriction Fragment Length
    • Restriction Mapping
    • Species Specificity

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    Citations

    This article has been cited 8 times.
    1. Badr C, Souiai O, Arbi M, El Behi I, Essaied MS, Khosrof I, Benkahla A, Chabchoub A, Ghram A. Epidemiological and Phylogeographic Study of Equid Herpesviruses in Tunisia.. Pathogens 2022 Sep 5;11(9).
      doi: 10.3390/pathogens11091016pubmed: 36145448google scholar: lookup
    2. Onasanya AE, El-Hage C, Diaz-Méndez A, Vaz PK, Legione AR, Browning GF, Devlin JM, Hartley CA. Whole genome sequence analysis of equid gammaherpesvirus -2 field isolates reveals high levels of genomic diversity and recombination.. BMC Genomics 2022 Aug 30;23(1):622.
      doi: 10.1186/s12864-022-08789-xpubmed: 36042397google scholar: lookup
    3. Marenzoni ML, Stefanetti V, Danzetta ML, Timoney PJ. Gammaherpesvirus infections in equids: a review.. Vet Med (Auckl) 2015;6:91-101.
      doi: 10.2147/VMRR.S39473pubmed: 30155436google scholar: lookup
    4. Wilkie GS, Kerr K, Stewart JP, Studdert MJ, Davison AJ. Genome sequences of equid herpesviruses 2 and 5.. Genome Announc 2015 Mar 12;3(2).
      doi: 10.1128/genomeA.00119-15pubmed: 25767243google scholar: lookup
    5. Browning GF, Studdert MJ. Physical mapping of a genome of equine herpesvirus 2 (equine cytomegalovirus).. Arch Virol 1989;104(1-2):77-86.
      doi: 10.1007/BF01313809pubmed: 2923549google scholar: lookup
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    7. Bublot M, Wellemans G, Van Bressem MF, Dubuisson J, Pastoret PP, Thiry E. Genomic diversity among bovine herpesvirus 4 field isolates.. Arch Virol 1991;116(1-4):1-18.
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    8. Roizmann B, Desrosiers RC, Fleckenstein B, Lopez C, Minson AC, Studdert MJ. The family Herpesviridae: an update. The Herpesvirus Study Group of the International Committee on Taxonomy of Viruses.. Arch Virol 1992;123(3-4):425-49.
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