Physiological adjustments of haematological profile during the last trimester of pregnancy and the early post partum period in mares.

This research study investigates the alterations in certain blood parameters in mares during the last trimester of their pregnancy and the first few weeks after giving birth. The study uses comparisons and analysis of blood samples to reveal the physiological changes mares undergo in these critical periods.

Objective of the Study

• The primary aim of this research was to scrutinize the changes in various haematological parameters of mares in the period encompassing the last three months of pregnancy and up until the third week postpartum.

Methodology of the Study

• For the purpose of the study, two groups of healthy mares were analyzed: Group A consisted of 15 pregnant mares, and Group B consisted of 7 non-pregnant, non-lactating mares.
• The researchers collected blood samples from these mares using a procedure known as jugular venipuncture, for which the blood was stored in EDTA tubes.
• These samples were then tested for several parameters including Haematocrit (Hct), Red Blood Cells (RBC), Haemoglobin (Hb), Platelets (Plt) as well as White Blood Cells (WBC).
• Additionally, peripheral blood smears were performed to understand the changes in the leucocyte differential count.

Results of the Study

• The results showed that Group A (pregnant mares) exhibited a gradual decline in their Hct and Hb levels throughout the experimental time period.
• The levels of Platelets and White Blood Cells, however, reached their peak at the time of parturition.
• Of the WBCs, neutrophils demonstrated an upward trend until birth, during which lymphocytes hit their lowest values.
• By implementing statistical analysis, the researchers found significant time × pregnancy interactions on Hb, WBCs, neutrophils, and lymphocytes.

Conclusion of the Study

• The study offers valuable insights into how the period surrounding parturition affects the haematological parameters in mares, by analyzing their physiological adjustments during the last trimester of pregnancy and the initial postpartum phase.