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Home / Equine Research Bank / Muscles Ligaments Tendons J / Pilot experimental study on amniotic epithelial mesenchymal ...
Muscles, ligaments and tendons journal2015; 5(1); 5-11;

Pilot experimental study on amniotic epithelial mesenchymal cell transplantation in natural occurring tendinopathy in horses. Ultrasonographic and histological comparison.

Abstract: amnion-derived stem cells are considered a promising alternative source for tendon tissue regeneration. Objective: aims of this paper were to illustrate the ultrasound and histological outcomes following the treatment of acute and chronic superficial digital flexor tendon spontaneous lesions in horses with ovine amniotic epithelial cells xenotransplantation. Methods: six adult horses suffering from unilateral acute (4 cases) and chronic (2 cases) tendinopathy (clinical and ultrasound diagnosis) were enrolled. At baseline, ovine amniotic epithelial cells were grafted, in sterile conditions and under ultra-sound control, into the most damaged area. Ultra-sound controls were performed at 30, 60, 90, 120, 150 and 180 days after cells implantation; after horse euthanasia (180 days) tendon samples were collected and submitted to histological examination (cellularity, extracellular matrix fiber organization, blood vessels). Results: at baseline, in the acute cases, the ultra-sound exam showed a focal, dis-homogeneous, hypo-echoic area into the superficial digital flexor tendon, with loss of the normal fibrillar pattern, while in the chronic cases the damaged tendon area appeared thickened and completely hyper-echoic. At the final follow-up tendon echotexture was more regular, the cross-sectional area similar to the contra-lateral limb, and the collagen fibers were oriented in parallel to the longitudinal axis of the tendon both in the acute and chronic cases, suggesting a positive healing response. These findings were supported by the histological analyses which showed an almost complete restoration of normal tendon architecture with an optimal alignment of tendon fibers. Conclusions: the present pilot study supports the hypothesis that amniotic epithelial cells are provided of an excellent healing potential and shows a very good correlation between the ultrasound findings and the histologic features.
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Publication Date: 2015-03-27 PubMed ID: 25878980PubMed Central: PMC4396678
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Summary

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This research aims to explore the effect of transplanting ovine amniotic epithelial cells in treating acute and chronic tendinopathy in horses, with evaluation based on ultrasounds and histological examinations. Initial findings suggest a positive correlation between the use of these cells and the healing process.

Objective

The aim of the research was focused on assessing the ultrasound and histological outcomes of treating naturally occurring acute and chronic tendon lesions in horses through xenotransplantation of ovine amniotic epithelial cells (stem cells found in the amniotic membrane of a sheep’s placenta). The researchers investigated the healing potentials of these cells in tendons tissue regeneration by looking closely at any changes in the tendon’s appearance under ultra-sounds, and studying the arrangement and organization of cells and fibers within the tendon tissue in a histologic examination.

Methodology

  • Six adult horses, suffering from either unilateral acute or chronic tendinopathy, were enrolled in the study.
  • The cells in question were transplanted into the most damaged area of the tendons in a sterile manner and under ultrasound control.
  • These horses were then subjected to ultrasound controls at different intervals throughout the 180-day study period.
  • After the horses were put down, their tendon samples were collected to evaluate cellularity, the organization of the extracellular matrix fiber, and blood vessels via histological examinations.

Results

  • At baseline, ultrasound scans showed distinct differences between the acute and chronic cases. Acute cases showed a focal, dis-homogeneous, hypo-echoic area in the tendon while chronic cases showed a thickened and completely hyper-echoic damaged tendon area.
  • As the study progressed, ultrasounds showed progress in healing, with the cross-sectional area of the tendon gradually becoming similar to that of the opposite and healthier limb. Furthermore, the collagen fibers started orienting parallel to the tendon’s longitudinal axis, indicating a positive healing response.
  • Results were corroborated by histological analyses. Tendon architecture seemed to return to its normal state after treatment, showing an optimal alignment of tendon fibers indicative of healing and tissue regeneration.

Conclusion

The study’s findings encourage the researchers’ initial hypothesis that amniotic epithelial cells have excellent healing potential. There was a noteworthy correlation between the ultrasound findings and the histologic features, both suggesting a positive response to the transplanted ovine amniotic epithelial cells in treating tendinopathy.

Cite This Article

APA
(2015). Pilot experimental study on amniotic epithelial mesenchymal cell transplantation in natural occurring tendinopathy in horses. Ultrasonographic and histological comparison. Muscles Ligaments Tendons J, 5(1), 5-11.

Publication

Muscles, ligaments and tendons journal
ISSN: 2240-4554
NlmUniqueID: 101600853
Country: Italy
Language: English
Volume: 5
Issue: 1
Pages: 5-11

Researcher Affiliations

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Citations

This article has been cited 13 times.
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