Plasma concentrations of 15-ketodihydro-PGF(2alpha), cortisol and progesterone during manual twin reduction in thoroughbred mares.
Abstract: The aim of the present study was to highlight the effect of two different techniques of one embryo crushing on some hormonal changes. Ten twinning mares were submitted to the mobile or fixed manual crushing of one blastocyst within day 19 after the last mating. Blood sample was collected from 20 min before to 90 min, 24 and 72 h after the procedure was performed to analyse 15-ketodihydro-PGF(2alpha), cortisol and progesterone plasma concentrations. Singleton pregnancy diagnosis was checked 72 h after crushing and at term of pregnancy. Because the unwanted crushing of both embryos occurred in one mare during the attempt of manual separation of the twins, that mare was not included in the evaluation of crushing-induced hormonal changes. No significant differences in hormonal concentrations were observed after one embryo crushing and also when the effect of the mobile (n = 6) or fixed (n = 3) technique was specifically evaluated. When the effect of the two techniques on each post-crushing sampling time hormonal levels was analysed, only a higher cortisol level 30 min after the fixed compared with the mobile technique was observed. The crushing performed within 19 days of gestation does not induce significant changes in 15-ketodihydro-PGF(2alpha), cortisol or progesterone plasma concentrations. When the fixed technique was performed, only a temporary higher cortisol concentration was seen 30 min after crushing, suggesting that the fixed technique might be responsible for a slight level of stress for the mare.
Publication Date: 2005-09-24 PubMed ID: 16176572DOI: 10.1111/j.1439-0442.2005.00748.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research paper examines the hormonal changes that occurred in mares during the manual twin reduction process, comparing two different techniques of crushing one embryo within 19 days of the last mating. It was observed that these processes had no significant effect on plasma concentrations of 15-ketodihydro-PGF(2alpha), cortisol, or progesterone, though a slight increase in cortisol levels was noted 30 minutes following the fixed technique of crushing, which may indicate some level of stress in the mare.
Objective of the Study
- The research aimed to identify the hormonal changes in mares during manual twin reduction via mobile and fixed techniques of one embryo crushing performed within 19 days following the last mating.
Methodology
- The randomized study was conducted on ten twinning mares, with blood samples collected before and after the procedure at specific intervals (20 min prior to, 90 min, 24 h, and 72 h after performing the procedure).
- The examined hormones were 15-ketodihydro-PGF(2alpha), cortisol, and progesterone.
Exclusions
- One mare, where the unwanted crushing of both embryos occurred during an attempt to manually separate the twins, was not considered in the evaluation of induced hormonal changes due to the crushing procedure.
Findings
- No significant changes in hormonal concentrations were observed after the crushing of one embryo, regardless of whether the mobile or fixed method was used.
- However, a higher cortisol level was reported 30 minutes after using the fixed technique compared to the mobile one, suggesting the possibility of a slight stress influence on the mare from the fixed technique.
- The conclusion of the study is that crushing performed within 19 days of gestation does not noticeably affect the plasma concentrations of the studied hormones (15-ketodihydro-PGF(2alpha), cortisol, or progesterone).
Implications
- The results of this study could serve as a guide for practitioners in selecting the appropriate twin reduction method in mares, given the potential for a small increase in stress following the fixed crushing technique.
Cite This Article
APA
Veronesi MC, Faustini M, Villani M, Kindahl H, Galeati G, Battocchio M.
(2005).
Plasma concentrations of 15-ketodihydro-PGF(2alpha), cortisol and progesterone during manual twin reduction in thoroughbred mares.
J Vet Med A Physiol Pathol Clin Med, 52(8), 411-415.
https://doi.org/10.1111/j.1439-0442.2005.00748.x Publication
Researcher Affiliations
- Dipartimento di Scienze Cliniche Veterinarie, Sez. Clinica Ostetrica e Ginecologica Veterinaria, Milan, Italy. maria.veronesi@unimi.it
MeSH Terms
- Animals
- Dinoprost / analogs & derivatives
- Dinoprost / blood
- Female
- Horses / blood
- Horses / physiology
- Hydrocortisone / blood
- Pregnancy
- Pregnancy Reduction, Multifetal / adverse effects
- Pregnancy Reduction, Multifetal / veterinary
- Progesterone / blood
- Stress, Physiological / blood
- Stress, Physiological / etiology
- Stress, Physiological / veterinary
- Twins
Citations
This article has been cited 1 times.- Raggio I, Lefebvre RC, Poitras P, Vaillancourt D, Goff AK. Twin pregnancy experimental model for transvaginal ultrasound-guided twin reduction in mares.. Can Vet J 2008 Nov;49(11):1093-8.
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