Plasma Estradiol Profile After Administration of Different Types of Estradiol Esters in Acyclic Mares.
Abstract: Although the use of different estradiol esters has been extensively studied in hormonal protocols in cows, such information is lacking in mares. The present study aimed to assess the effects of treatment with the same doses and administration frequency of estradiol cypionate, estradiol benzoate and 17β estradiol on plasma estradiol (E2) concentrations of acyclic mares and correlate the E2 profile to the endometrial edema score. Sixteen treatments were performed in 14 mares randomly divided into three groups: EB (n = 5), EC (n = 5), and 17β (n = 6), receiving 10 mg on day 0 (D0), 6 mg on D1, and 4 mg on D2 of estradiol benzoate, estradiol cypionate, and estradiol 17β, respectively. Blood samples, rectal palpations, and ultrasound evaluations were performed once daily, starting before the first estradiol treatment (D0) until edema disappearance or D8. Moderate to high edema was observed in all groups 24 hours after the first estradiol administration. Edema persisted above score 2 until D7, D5, and D4 in groups EC, EB, and 17β, respectively. Higher edema was found on D2 in EB group compared to 17β, on D6 and D7 in EC compared to 17β, and on D8 in EC compared to EB (p ≤ .05). Maximum E2 concentrations were detected on D1 in groups: EB, showing a sharp decrease from D2 to D3 (p < .0001); and 17β, where no differences were observed between treatment days (p ≥ .05). In the EC group, maximum concentrations were observed on D2, which remained high from D2 to D4 compared to the other days (p < .0001). Plasma concentration of E2 was higher in EB than the other groups on D0 and D1, and EB>EC>17β on D2. Plasma E2 concentrations and edema score were positively correlated, being moderate in groups EB and EC and weak in the 17β group. In conclusion, the most pronounced plasma E2 concentration was observed in EB group 24 hours after the administration of the first dose. Estradiol concentrations peaked 48 hours after EC administration, while a distinct peak was not observed in a 24h interval evaluation in the 17β group. Moreover, high edema does not necessarily reflect on high E2 concentrations in acyclic mares.
Copyright © 2023 Elsevier Inc. All rights reserved.
Publication Date: 2023-05-27 PubMed ID: 37247749DOI: 10.1016/j.jevs.2023.104824Google Scholar: Lookup
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- Randomized Controlled Trial
- Veterinary
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research aims to understand how the treatment with different types of estradiol esters, specifically estradiol cypionate, estradiol benzoate, and 17β estradiol, affects plasma estradiol concentrations and endometrial edema in acyclic mares.
Research Methodology
- The study included 14 mares selected randomly and divided into three groups: EB (n = 5), EC (n = 5), and 17β (n = 6). Each group received different types of estradiol esters, with 10 mg administered on day 0 (D0), 6 mg on D1, and 4 mg on D2.
- The research team performed daily blood samples, rectal palpations, and ultrasound evaluations from the first day of estradiol treatment (D0) until the disappearance of edema or by D8.
Key Findings
- 24 hours after the first estradiol treatment, all groups showed moderate to high edema. This condition persisted beyond score 2 until D7 for group EC, D5 for EB, and D4 for the 17β group.
- The EB group exhibited higher edema on D2 compared to the 17β group, while the EC group had higher scores on D6 and D7 compared to the 17β group, and on D8 compared to the EB group.
- Maximum E2 concentrations differed across the groups. In the EB group, they were detected on D1, showing a sharp decrease from D2 to D3, while in the 17β group, maximum concentrations were observed with no significant differences between treatment days. For the EC group, maximum concentrations were seen on D2, which remained high from D2 to D4 compared to the other days.
- Plasma E2 concentrations were higher in the EB group on D0 and D1, and followed the sequence EB>EC>17β on D2.
- There was a positive correlation between plasma E2 concentrations and edema score, with it being moderate in groups EB and EC and weak in the 17β group.
Conclusions
- Following the first estradiol dose administration, the most severe plasma E2 concentrations were observed in the EB group after 24 hours.
- Estradiol concentration peaks were observed 48 hours after EC administration, while a distinct peak wasn’t observed in the 24h interval evaluation in the 17β group.
- High edema doesn’t necessarily indicate high E2 concentrations in acyclic mares.
Cite This Article
APA
Setoguchi LS, Siqueira LM, Lucca LK, Oquendo PS, Oquendo FMG, Nogueira GP, Silva ESM.
(2023).
Plasma Estradiol Profile After Administration of Different Types of Estradiol Esters in Acyclic Mares.
J Equine Vet Sci, 127, 104824.
https://doi.org/10.1016/j.jevs.2023.104824 Publication
Researcher Affiliations
- School of Veterinary Medicine, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
- School of Veterinary Medicine, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
- School of Veterinary Medicine, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
- School of Veterinary Medicine, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
- School of Veterinary Medicine, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
- School of Veterinary Medicine, São Paulo State University (UNESP) - Araçatuba, São Paulo, Brazil.
- School of Veterinary Medicine, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil. Electronic address: elisasmsilva@ufu.br.
MeSH Terms
- Female
- Horses
- Animals
- Cattle
- Estradiol / pharmacology
- Ultrasonography
Conflict of Interest Statement
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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