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Journal of veterinary internal medicine2015; 30(1); 260-268; doi: 10.1111/jvim.13811

Plasma HMGB-1 and Nucleosome Concentrations in Horses with Colic and Healthy Horses.

Abstract: Acute gastrointestinal disease occurs commonly in horses. Novel biomarkers might improve the understanding of SIRS and aid diagnosis and determination of prognosis. Objective: Increased plasma concentrations of the biomarkers HMGB-1 and nucleosomes are associated with severity of gastrointestinal lesions in horses; concentrations of these biomarkers will be greater in horses with lesions more likely to cause SIRS; and will provide additional information compared with standard biomarkers fibrinogen and SAA. Methods: Thirty horses with gastrointestinal disease, 22 healthy horses. Methods: Prospective study. Plasma samples taken on admission were used for measurement of HMGB-1, nucleosomes, fibrinogen, and SAA. Values were compared between healthy horses and those with gastrointestinal disease, and between horses with gastrointestinal disease grouped by lesion type (inflammatory, strangulating, and nonstrangulating). Correlations between biomarkers were assessed. Results: Plasma concentrations of all biomarkers were significantly higher in horses with gastrointestinal disease compared to healthy horses (P ≤ .001). HMGB-1 and nucleosomes were significantly higher in inflammatory and strangulating groups compared to healthy horses (3.5-fold and 5.4-fold increases, respectively, for HMGB-1 (P < .05) and 4.8-fold and 5.6-fold increases for nucleosomes (P < .05)), but concentrations in the group with nonstrangulating disease did not differ from healthy horses. There was significant correlation between HMGB-1 and nucleosomes (Spearman's r = 0.623; P < .001), and fibrinogen and SAA (Spearman's r = 0.801; P < .001) but not between other biomarkers. Conclusions: High mobility group box-1 and nucleosomes might have use as biomarkers for horses with gastrointestinal disease. Further studies are required to determine kinetics and prognostic value of serial measurements of these biomarkers in horses.
Copyright © 2015 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.
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Publication Date: 2015-12-18 PubMed ID: 26683003PubMed Central: PMC4913630DOI: 10.1111/jvim.13811Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article focuses on the investigation of plasma concentrations of biomarkers HMGB-1 and nucleosomes in horses with gastrointestinal disease and comparing them with healthy horses. The study finds that these two markers might be valuable for diagnosing and understanding the severity of gastrointestinal conditions in horses.

Objective and Methodology

  • The objective of the study was to determine whether increased plasma concentrations of biomarkers HMGB-1 and nucleosomes are correlated with the severity of gastrointestinal lesions in horses. The authors postulated that these biomarkers would be more concentrated in horses with lesions likely to cause Systemic Inflammatory Response Syndrome (SIRS).
  • The study was conducted on a group of 30 horses with gastrointestinal disease and a control group of 22 healthy horses. The study was prospective, meaning the researchers planned and conducted analysis based on certain biomarkers before the onset of the study.
  • On admission, plasma samples were collected from all the horses for the measurement of HMGB-1, nucleosomes, fibrinogen, and serum amyloid A (SAA). These values were then compared between the healthy horses and those with gastrointestinal disease.
  • The horses with gastrointestinal disease were further grouped by lesion type – inflammatory, strangulating and non-strangulating. This allowed detailed comparisons of biomarker concentrations among diverse lesion types.

Results

  • The study concluded that plasma concentrations of all the studied biomarkers were significantly higher in horses with gastrointestinal disease compared to healthy horses.
  • The two primary investigated biomarkers, HMGB-1 and nucleosomes, displayed substantially elevated levels in horses with inflammatory and strangulating lesions compared to healthy horses. However, they did not observe any significant difference in biomarker concentration in the group with non-strangulating disease when compared with healthy horses.
  • Moreover, the researchers found a significant correlation between HMGB-1 and nucleosomes, and between fibrinogen and SAA, which indicates a relation between the severity of inflammation (measured by fibrinogen and SAA) and cell death (measured by HMGB-1 and nucleosomes).

Conclusion and Further Research

  • The study concluded that High mobility group box-1 (HMGB-1) and nucleosomes might be useful as biomarkers for horses with gastrointestinal disease, contributing to improved diagnosis and prognosis determinations.
  • However, the authors also stress that further studies are required to determine the kinetics and prognostic value of serial measurements of these biomarkers in horses. The serial measurements will help to understand how these biomarker concentrations change over the course of disease progression and treatment.

Cite This Article

APA
Bauquier JR, Forbes G, Nath L, Tudor E, Bailey SR. (2015). Plasma HMGB-1 and Nucleosome Concentrations in Horses with Colic and Healthy Horses. J Vet Intern Med, 30(1), 260-268. https://doi.org/10.1111/jvim.13811

Publication

Journal of veterinary internal medicine
ISSN: 1939-1676
NlmUniqueID: 8708660
Country: United States
Language: English
Volume: 30
Issue: 1
Pages: 260-268

Researcher Affiliations

Bauquier, J R
  • Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Parkville, Vic., Australia.
Forbes, G
  • Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Parkville, Vic., Australia.
Nath, L
  • Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Parkville, Vic., Australia.
Tudor, E
  • Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Parkville, Vic., Australia.
Bailey, S R
  • Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Parkville, Vic., Australia.

MeSH Terms

  • Animals
  • Biomarkers
  • Colic / blood
  • Colic / metabolism
  • Colic / veterinary
  • Female
  • Gene Expression Regulation / physiology
  • HMGB1 Protein / blood
  • HMGB1 Protein / genetics
  • HMGB1 Protein / metabolism
  • Horse Diseases / blood
  • Horse Diseases / metabolism
  • Horses
  • Male
  • Nucleosomes / metabolism
  • Systemic Inflammatory Response Syndrome / blood
  • Systemic Inflammatory Response Syndrome / veterinary

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Citations

This article has been cited 6 times.
  1. Goggs R, Robbins SN, LaLonde-Paul DM, Menard JM. Serial analysis of blood biomarker concentrations in dogs with pneumonia, septic peritonitis, and pyometra. J Vet Intern Med 2022 Mar;36(2):549-564.
    doi: 10.1111/jvim.16374pubmed: 35103342google scholar: lookup
  2. Goggs R. Effect of sample type on plasma concentrations of cell-free DNA and nucleosomes in dogs. Vet Rec Open 2019;6(1):e000357.
    doi: 10.1136/vetreco-2019-000357pubmed: 31673376google scholar: lookup
  3. Martiny P, Goggs R. Biomarker Guided Diagnosis of Septic Peritonitis in Dogs. Front Vet Sci 2019;6:208.
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  4. Letendre JA, Goggs R. Concentrations of Plasma Nucleosomes but Not Cell-Free DNA Are Prognostic in Dogs Following Trauma. Front Vet Sci 2018;5:180.
    doi: 10.3389/fvets.2018.00180pubmed: 30105230google scholar: lookup
  5. Goggs R, Letendre JA. High Mobility Group Box-1 and Pro-inflammatory Cytokines Are Increased in Dogs After Trauma but Do Not Predict Survival. Front Vet Sci 2018;5:179.
    doi: 10.3389/fvets.2018.00179pubmed: 30105229google scholar: lookup
  6. Birckhead EM, Das S, Tidd N, Raidal SL, Raidal SR. Visualizing neutrophil extracellular traps in septic equine synovial and peritoneal fluid samples using immunofluorescence microscopy. J Vet Diagn Invest 2023 Nov;35(6):751-760.
    doi: 10.1177/10406387231196552pubmed: 37661696google scholar: lookup
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