Plasma lysozyme activity of Polish primitive horses under physiological conditions and in experimental fever.
Abstract: The normal level of plasma lysozyme of 9 Tarpane-like horses was 0.922pg/ml. This was
calculated from determinations performed 5 times in a period of 14 months. Observations on the
normal plasma level of lysozyme in the horse revealed marked variations in individual animals, as well
as in the whole group and in the mean values calculated for individual horses. The model for the
subsequent studies was an experimental fever evoked by the administration of E. colz LPS in a dose of
0.1 pg/kg body weight. Blood samples from the jugular vein were taken just before LPS injection, and
then for 8 h at 1 h intervals. Additional samples were collected at 24 and 48 h after LPS. Experiments
were repeated three times in 3 seasons of the year. Rectal temperature, plasma lysozyme activity and
leucocyte counts were recorded during fever. The following shifts of the measured parameters were
statistically significant: decrease in leucocyte number with the lowest value at 2 h (from 8,422 f 1,773
to 4,440 f 1,488 cells/mm'), increase in plasma lysozyme level with the peak at 3 h (from
0.904 k 0.401 to 1.440 k 0.748 pg/ml) and a temperature rise which peaked at 4 h (increase 1.01 "C)
after LPS iniection.
Publication Date: 1987-12-01 PubMed ID: 3124401DOI: 10.1111/j.1439-0442.1987.tb00345.xGoogle Scholar: Lookup
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- Journal Article
Summary
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This study examines the plasma lysozyme activity variability in Polish primitive horses under normal conditions as well as during induced fever experiments. The researchers measured significant decreases in leucocyte number, an increase in plasma lysozyme levels, and an associated rise in body temperature.
Study Objective and Methodology
- The intentional purpose of this study sought to understand the normal level of plasma lysozyme in Polish primitive horses, specifically the Tarpane-like breed, under standard physiological conditions and during periods of artificially induced fever.
- Researchers calculated the normal level of plasma lysozyme from measurements taken five times over a 14-month period from a group of nine horses.
- They noticed significant fluctuations between individual animals and even within a single horse’s values over time.
- This study’s experimental setup involved inducing a fever in the horses using Escherichia coli Lipopolysaccharides (E.coli LPS), a type of bacterial endotoxin, at a dose of 0.1 pg/kg body weight.
- Blood samples were taken from the jugular vein just before and after LPS injection at various intervals – one hour intervals for the initial eight hours, and additional samples collected at 24 and 48 hours.
Findings
- The experiments were replicated three times across different seasons to factor in seasonal variability.
- The researchers recorded three primary changes in the measured parameters: a significant reduction in leucocyte count with the lowest value appearing two hours after LPS injection; a substantial increase in plasma lysozyme levels reaching its peak three hours post-injection; and a correlated rise in body temperature peaking four hours after the LPS injection.
- This research strongly indicates that the plasma lysozyme activity in horses, especially under induced fever conditions, shows significant variations, presenting an essential dynamic in understanding their immune responses and physiological reactions to infections.
Cite This Article
APA
Wiśniewski E, Kuźma K.
(1987).
Plasma lysozyme activity of Polish primitive horses under physiological conditions and in experimental fever.
Zentralbl Veterinarmed A, 34(10), 776-781.
https://doi.org/10.1111/j.1439-0442.1987.tb00345.x Publication
Researcher Affiliations
MeSH Terms
- Animals
- Female
- Horse Diseases / enzymology
- Horses / blood
- Lipopolysaccharides / administration & dosage
- Male
- Muramidase / blood
- Reference Values
- Shock, Septic / enzymology
- Shock, Septic / veterinary
Citations
This article has been cited 1 times.- Bruhn O, Grötzinger J, Cascorbi I, Jung S. Antimicrobial peptides and proteins of the horse--insights into a well-armed organism. Vet Res 2011 Sep 2;42(1):98.
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